2FCH
Crystal Structure of Thioredoxin Mutant G74S
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0015035 | molecular_function | protein-disulfide reductase activity |
| A | 0030337 | molecular_function | DNA polymerase processivity factor activity |
| A | 0045454 | biological_process | cell redox homeostasis |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0015035 | molecular_function | protein-disulfide reductase activity |
| B | 0030337 | molecular_function | DNA polymerase processivity factor activity |
| B | 0045454 | biological_process | cell redox homeostasis |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0015035 | molecular_function | protein-disulfide reductase activity |
| C | 0030337 | molecular_function | DNA polymerase processivity factor activity |
| C | 0045454 | biological_process | cell redox homeostasis |
| D | 0005515 | molecular_function | protein binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0015035 | molecular_function | protein-disulfide reductase activity |
| D | 0030337 | molecular_function | DNA polymerase processivity factor activity |
| D | 0045454 | biological_process | cell redox homeostasis |
| E | 0005515 | molecular_function | protein binding |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005829 | cellular_component | cytosol |
| E | 0015035 | molecular_function | protein-disulfide reductase activity |
| E | 0030337 | molecular_function | DNA polymerase processivity factor activity |
| E | 0045454 | biological_process | cell redox homeostasis |
| F | 0005515 | molecular_function | protein binding |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0005829 | cellular_component | cytosol |
| F | 0015035 | molecular_function | protein-disulfide reductase activity |
| F | 0030337 | molecular_function | DNA polymerase processivity factor activity |
| F | 0045454 | biological_process | cell redox homeostasis |
| G | 0005515 | molecular_function | protein binding |
| G | 0005737 | cellular_component | cytoplasm |
| G | 0005829 | cellular_component | cytosol |
| G | 0015035 | molecular_function | protein-disulfide reductase activity |
| G | 0030337 | molecular_function | DNA polymerase processivity factor activity |
| G | 0045454 | biological_process | cell redox homeostasis |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MPD D 501 |
| Chain | Residue |
| B | TYR70 |
| D | TYR70 |
| D | GLY71 |
| D | THR89 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MPD E 502 |
| Chain | Residue |
| E | ILE60 |
| E | ALA67 |
| E | ARG73 |
| E | SER74 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MPD E 503 |
| Chain | Residue |
| A | THR89 |
| E | TYR70 |
| E | GLY71 |
| E | THR89 |
| A | TYR70 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE MPD G 504 |
| Chain | Residue |
| F | TRP31 |
| F | ILE60 |
| F | ILE72 |
| F | ARG73 |
| F | SER74 |
| G | TYR70 |
| G | GLY71 |
| G | HOH508 |
| site_id | AC5 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE MPD D 505 |
| Chain | Residue |
| B | ARG73 |
| B | HOH114 |
| B | HOH115 |
| C | ILE41 |
| C | GLU44 |
| C | LYS96 |
| C | HOH120 |
| D | TYR70 |
| D | GLY84 |
| D | GLU85 |
| D | VAL86 |
| D | HOH507 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MPD D 506 |
| Chain | Residue |
| D | ILE60 |
| D | ILE72 |
| D | ARG73 |
| E | LYS36 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MPD A 507 |
| Chain | Residue |
| A | ILE72 |
| A | HOH509 |
| B | LYS36 |
| B | PRO40 |
Functional Information from PROSITE/UniProt
| site_id | PS00194 |
| Number of Residues | 19 |
| Details | THIOREDOXIN_1 Thioredoxin family active site. LVdFWaeWCGPCKmIapiL |
| Chain | Residue | Details |
| A | LEU24-LEU42 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 14 |
| Details | Active site: {"description":"Nucleophile"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 7 |
| Details | Site: {"description":"Deprotonates C-terminal active site Cys"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 14 |
| Details | Site: {"description":"Contributes to redox potential value"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 7 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| A | PRO34 | |
| A | CYS35 | |
| A | CYS32 | |
| A | GLY33 |
| site_id | CSA10 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| C | CYS35 | |
| C | CYS32 |
| site_id | CSA11 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| D | CYS35 | |
| D | CYS32 |
| site_id | CSA12 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| E | CYS35 | |
| E | CYS32 |
| site_id | CSA13 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| F | CYS35 | |
| F | CYS32 |
| site_id | CSA14 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| G | CYS35 | |
| G | CYS32 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| B | PRO34 | |
| B | CYS35 | |
| B | CYS32 | |
| B | GLY33 |
| site_id | CSA3 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| C | PRO34 | |
| C | CYS35 | |
| C | CYS32 | |
| C | GLY33 |
| site_id | CSA4 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| D | PRO34 | |
| D | CYS35 | |
| D | CYS32 | |
| D | GLY33 |
| site_id | CSA5 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| E | PRO34 | |
| E | CYS35 | |
| E | CYS32 | |
| E | GLY33 |
| site_id | CSA6 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| F | PRO34 | |
| F | CYS35 | |
| F | CYS32 | |
| F | GLY33 |
| site_id | CSA7 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| G | PRO34 | |
| G | CYS35 | |
| G | CYS32 | |
| G | GLY33 |
| site_id | CSA8 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| A | CYS35 | |
| A | CYS32 |
| site_id | CSA9 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| B | CYS35 | |
| B | CYS32 |
