Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2FA9

The crystal structure of Sar1[H79G]-GDP provides insight into the coat-controlled GTP hydrolysis in the disassembly of COP II

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003924	molecular_function	GTPase activity
A	0003925	molecular_function	G protein activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005764	cellular_component	lysosome
A	0005765	cellular_component	lysosomal membrane
A	0005783	cellular_component	endoplasmic reticulum
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005794	cellular_component	Golgi apparatus
A	0005829	cellular_component	cytosol
A	0006886	biological_process	intracellular protein transport
A	0006888	biological_process	endoplasmic reticulum to Golgi vesicle-mediated transport
A	0015031	biological_process	protein transport
A	0016020	cellular_component	membrane
A	0016192	biological_process	vesicle-mediated transport
A	0016787	molecular_function	hydrolase activity
A	0030127	cellular_component	COPII vesicle coat
A	0032368	biological_process	regulation of lipid transport
A	0032580	cellular_component	Golgi cisterna membrane
A	0042953	biological_process	lipoprotein transport
A	0046872	molecular_function	metal ion binding
A	0048208	biological_process	COPII vesicle coating
A	0055088	biological_process	lipid homeostasis
A	0070971	cellular_component	endoplasmic reticulum exit site
A	0090110	biological_process	COPII-coated vesicle cargo loading
A	0140353	biological_process	lipid export from cell
A	0140785	molecular_function	amino acid sensor activity
A	1903432	biological_process	regulation of TORC1 signaling
A	1904262	biological_process	negative regulation of TORC1 signaling
A	1990253	biological_process	cellular response to leucine starvation
B	0000166	molecular_function	nucleotide binding
B	0003924	molecular_function	GTPase activity
B	0003925	molecular_function	G protein activity
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005737	cellular_component	cytoplasm
B	0005764	cellular_component	lysosome
B	0005765	cellular_component	lysosomal membrane
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0005794	cellular_component	Golgi apparatus
B	0005829	cellular_component	cytosol
B	0006886	biological_process	intracellular protein transport
B	0006888	biological_process	endoplasmic reticulum to Golgi vesicle-mediated transport
B	0015031	biological_process	protein transport
B	0016020	cellular_component	membrane
B	0016192	biological_process	vesicle-mediated transport
B	0016787	molecular_function	hydrolase activity
B	0030127	cellular_component	COPII vesicle coat
B	0032368	biological_process	regulation of lipid transport
B	0032580	cellular_component	Golgi cisterna membrane
B	0042953	biological_process	lipoprotein transport
B	0046872	molecular_function	metal ion binding
B	0048208	biological_process	COPII vesicle coating
B	0055088	biological_process	lipid homeostasis
B	0070971	cellular_component	endoplasmic reticulum exit site
B	0090110	biological_process	COPII-coated vesicle cargo loading
B	0140353	biological_process	lipid export from cell
B	0140785	molecular_function	amino acid sensor activity
B	1903432	biological_process	regulation of TORC1 signaling
B	1904262	biological_process	negative regulation of TORC1 signaling
B	1990253	biological_process	cellular response to leucine starvation

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B 1003

Chain	Residue
B	THR39
B	GDP1001
B	HOH2058
B	HOH2059
B	HOH2060

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG A 1004

Chain	Residue
A	HOH2044
A	HOH2063
A	HOH2064
A	THR39
A	PRO59
A	GDP1002
A	HOH2004

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 B 2001

Chain	Residue
B	PHE18
B	GLY185
B	GLY187
B	GLU188
B	ARG191
B	HOH2038
B	HOH2039

site_id	AC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 A 2002

Chain	Residue
A	PHE18
A	GLN184
A	GLY185
A	TYR186
A	GLY187
A	GLU188
A	ARG191
A	HOH2006
A	HOH2055

site_id	AC5
Number of Residues	20
Details	BINDING SITE FOR RESIDUE GDP B 1001

Chain	Residue
B	ASP34
B	ASN35
B	ALA36
B	GLY37
B	LYS38
B	THR39
B	THR40
B	ASP75
B	ASN134
B	LYS135
B	ASP137
B	ARG138
B	SER179
B	VAL180
B	LEU181
B	MG1003
B	HOH2026
B	HOH2035
B	HOH2059
B	HOH2060

site_id	AC6
Number of Residues	21
Details	BINDING SITE FOR RESIDUE GDP A 1002

Chain	Residue
A	ASP34
A	ASN35
A	ALA36
A	GLY37
A	LYS38
A	THR39
A	THR40
A	ASP75
A	ASN134
A	LYS135
A	ASP137
A	ARG138
A	SER179
A	VAL180
A	LEU181
A	MG1004
A	HOH2009
A	HOH2017
A	HOH2044
A	HOH2059
A	HOH2063

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269\|PubMed:11739406, ECO:0007744\|PDB:1F6B

Chain	Residue	Details
A	ASP34
A	ALA36
A	ASP75
B	ASP34
B	ALA36
B	ASP75

site_id	SWS_FT_FI2
Number of Residues	22
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:Q9Y6B6

Chain	Residue	Details
A	ASN35
A	VAL180
A	LEU181
B	ASN35
B	GLY37
B	LYS38
B	THR39
B	THR40
B	HIS58
B	ASN134
B	LYS135
A	GLY37
B	ASP137
B	VAL180
B	LEU181
A	LYS38
A	THR39
A	THR40
A	HIS58
A	ASN134
A	LYS135
A	ASP137

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:Q9Y6B6

Chain	Residue	Details
A	SER164
B	SER164

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
B	GLY79

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)