2EZT
Pyruvate oxidase variant F479W in complex with reaction intermediate 2-hydroxyethyl-thiamin diphosphate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0030976 | molecular_function | thiamine pyrophosphate binding |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0047112 | molecular_function | pyruvate oxidase activity |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0030976 | molecular_function | thiamine pyrophosphate binding |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0047112 | molecular_function | pyruvate oxidase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG A 1510 |
| Chain | Residue |
| A | ASP447 |
| A | ASN474 |
| A | GLN476 |
| A | TDM1611 |
| A | HOH2083 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NA B 1511 |
| Chain | Residue |
| B | HOH1682 |
| B | HOH1682 |
| B | MET452 |
| B | MET452 |
| B | GLN455 |
| B | GLN455 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PO4 A 1512 |
| Chain | Residue |
| A | GLY34 |
| A | GLY35 |
| A | SER36 |
| A | SER82 |
| A | GLN122 |
| A | ILE480 |
| A | TDM1611 |
| A | HOH1791 |
| A | HOH1964 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 1513 |
| Chain | Residue |
| B | ASP447 |
| B | ASN474 |
| B | GLN476 |
| B | TDM1614 |
| B | HOH2080 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NA A 1514 |
| Chain | Residue |
| A | MET452 |
| A | MET452 |
| A | GLN455 |
| A | GLN455 |
| A | HOH1688 |
| A | HOH1688 |
| site_id | AC6 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PO4 B 1515 |
| Chain | Residue |
| B | GLY34 |
| B | GLY35 |
| B | SER36 |
| B | SER82 |
| B | GLN122 |
| B | ILE480 |
| B | TDM1614 |
| B | HOH1734 |
| B | HOH2085 |
| site_id | AC7 |
| Number of Residues | 27 |
| Details | BINDING SITE FOR RESIDUE TDM A 1611 |
| Chain | Residue |
| A | PRO33 |
| A | GLU59 |
| A | SER82 |
| A | PRO85 |
| A | HIS89 |
| A | GLN122 |
| A | VAL394 |
| A | ASP396 |
| A | ALA420 |
| A | MET422 |
| A | GLY446 |
| A | ASP447 |
| A | GLY448 |
| A | GLY449 |
| A | ASN474 |
| A | GLN476 |
| A | TYR477 |
| A | GLY478 |
| A | TRP479 |
| A | ILE480 |
| A | MG1510 |
| A | PO41512 |
| A | FAD1612 |
| A | HOH1615 |
| A | HOH1647 |
| A | HOH1650 |
| A | HOH2083 |
| site_id | AC8 |
| Number of Residues | 32 |
| Details | BINDING SITE FOR RESIDUE FAD A 1612 |
| Chain | Residue |
| A | HIS101 |
| A | PHE121 |
| A | GLY220 |
| A | ILE221 |
| A | GLY222 |
| A | THR244 |
| A | TYR245 |
| A | PRO246 |
| A | ALA262 |
| A | ASN263 |
| A | ARG264 |
| A | VAL265 |
| A | GLY284 |
| A | ASN285 |
| A | ASN286 |
| A | PRO288 |
| A | PHE289 |
| A | ASP306 |
| A | ILE307 |
| A | ASP308 |
| A | LYS311 |
| A | ASP325 |
| A | ALA326 |
| A | ASN398 |
| A | SER416 |
| A | ASN417 |
| A | TDM1611 |
| A | HOH1630 |
| A | HOH1646 |
| A | HOH1661 |
| A | HOH1681 |
| A | HOH1911 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PYR A 1613 |
| Chain | Residue |
| A | LEU555 |
| A | LEU557 |
| A | ASP558 |
| A | SER562 |
| A | HOH1816 |
| A | HOH1831 |
| site_id | BC1 |
| Number of Residues | 28 |
| Details | BINDING SITE FOR RESIDUE TDM B 1614 |
| Chain | Residue |
| B | PRO33 |
| B | GLU59 |
| B | SER82 |
| B | PRO85 |
| B | HIS89 |
| B | GLN122 |
| B | VAL394 |
| B | GLY395 |
| B | ASP396 |
| B | ALA420 |
| B | MET422 |
| B | GLY446 |
| B | ASP447 |
| B | GLY448 |
| B | GLY449 |
| B | ASN474 |
| B | GLN476 |
| B | TYR477 |
| B | GLY478 |
| B | TRP479 |
| B | ILE480 |
| B | MG1513 |
| B | PO41515 |
| B | FAD1615 |
| B | HOH1620 |
| B | HOH1645 |
| B | HOH1653 |
| B | HOH2080 |
| site_id | BC2 |
| Number of Residues | 34 |
| Details | BINDING SITE FOR RESIDUE FAD B 1615 |
| Chain | Residue |
| B | HIS101 |
| B | PHE121 |
| B | GLY220 |
| B | ILE221 |
| B | GLY222 |
| B | THR244 |
| B | TYR245 |
| B | ALA262 |
| B | ASN263 |
| B | ARG264 |
| B | VAL265 |
| B | GLY284 |
| B | ASN285 |
| B | ASN286 |
| B | TYR287 |
| B | PRO288 |
| B | PHE289 |
| B | ASP306 |
| B | ILE307 |
| B | ASP308 |
| B | LYS311 |
| B | ALA324 |
| B | ASP325 |
| B | ALA326 |
| B | ASN398 |
| B | SER416 |
| B | ASN417 |
| B | TDM1614 |
| B | HOH1634 |
| B | HOH1635 |
| B | HOH1665 |
| B | HOH1674 |
| B | HOH1966 |
| B | HOH2055 |
| site_id | BC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PYR B 1616 |
| Chain | Residue |
| B | LEU555 |
| B | ARG556 |
| B | LEU557 |
| B | ASP558 |
| B | MET561 |
| B | SER562 |
| B | HOH1948 |
Functional Information from PROSITE/UniProt
| site_id | PS00187 |
| Number of Residues | 20 |
| Details | TPP_ENZYMES Thiamine pyrophosphate enzymes signature. IAaklnyPerqvFnLaGDGG |
| Chain | Residue | Details |
| A | ILE430-GLY449 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 300 |
| Details | Region: {"description":"FAD-binding"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | Binding site: {} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1dtw |
| Chain | Residue | Details |
| A | ALA552 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1dtw |
| Chain | Residue | Details |
| B | ALA552 |
| site_id | CSA3 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dtw |
| Chain | Residue | Details |
| A | ARG264 | |
| A | TRP479 | |
| A | GLU483 |
| site_id | CSA4 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1dtw |
| Chain | Residue | Details |
| B | ARG264 | |
| B | TRP479 | |
| B | GLU483 |
| site_id | CSA5 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1dtw |
| Chain | Residue | Details |
| A | MET561 |
| site_id | CSA6 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1dtw |
| Chain | Residue | Details |
| B | MET561 |
| site_id | MCSA1 |
| Number of Residues | 8 |
| Details | M-CSA 274 |
| Chain | Residue | Details |
| A | GLU59 | activator, hydrogen bond acceptor, increase acidity, proton acceptor, proton donor |
| A | PHE121 | electrostatic destabiliser, polar/non-polar interaction, promote heterolysis, radical stabiliser |
| A | GLN122 | activator, electrostatic destabiliser, hydrogen bond acceptor, hydrogen bond donor, promote heterolysis |
| A | ARG264 | radical stabiliser |
| A | VAL394 | polar/non-polar interaction, promote heterolysis, radical stabiliser, steric role |
| A | TRP479 | electrostatic destabiliser, polar/non-polar interaction, promote heterolysis, radical stabiliser |
| A | ILE480 | electrostatic destabiliser, polar/non-polar interaction, promote heterolysis, radical stabiliser |
| A | GLU483 | radical stabiliser |
| site_id | MCSA2 |
| Number of Residues | 8 |
| Details | M-CSA 274 |
| Chain | Residue | Details |
| B | GLU59 | activator, hydrogen bond acceptor, increase acidity, proton acceptor, proton donor |
| B | PHE121 | electrostatic destabiliser, polar/non-polar interaction, promote heterolysis, radical stabiliser |
| B | GLN122 | activator, electrostatic destabiliser, hydrogen bond acceptor, hydrogen bond donor, promote heterolysis |
| B | ARG264 | radical stabiliser |
| B | VAL394 | polar/non-polar interaction, promote heterolysis, radical stabiliser, steric role |
| B | TRP479 | electrostatic destabiliser, polar/non-polar interaction, promote heterolysis, radical stabiliser |
| B | ILE480 | electrostatic destabiliser, polar/non-polar interaction, promote heterolysis, radical stabiliser |
| B | GLU483 | radical stabiliser |
