2EWE

Crystal structure of Pectate Lyase C R218K mutant in complex with pentagalacturonic acid

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005576	cellular_component	extracellular region
A	0016829	molecular_function	lyase activity
A	0030570	molecular_function	pectate lyase activity
A	0045490	biological_process	pectin catabolic process
A	0046872	molecular_function	metal ion binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: ACT_SITE => ECO:0000255

Chain	Residue	Details
A	LYS218

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site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:12540845

Chain	Residue	Details
A	ASP129
A	ASP131
A	GLU166
A	ASP170

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 2pec

Chain	Residue	Details
A	ASP131
A	LYS218

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site_id	MCSA1
Number of Residues	5
Details	M-CSA 896

Chain	Residue	Details
A	ASP129	metal ligand
A	ASP131	metal ligand
A	GLU166	metal ligand
A	ASP170	metal ligand
A	LYS218	modifies pKa, proton shuttle (general acid/base)

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