2EII
Crystal analysis of delta1-pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus with bound L-valine and NAD.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0003842 | molecular_function | 1-pyrroline-5-carboxylate dehydrogenase activity |
| A | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| A | 0010133 | biological_process | proline catabolic process to glutamate |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0003842 | molecular_function | 1-pyrroline-5-carboxylate dehydrogenase activity |
| B | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| B | 0010133 | biological_process | proline catabolic process to glutamate |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT A 1519 |
| Chain | Residue |
| A | ALA245 |
| A | GLU249 |
| A | ILE268 |
| A | ALA271 |
| A | NAD1518 |
| A | HOH2863 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ACT A 1520 |
| Chain | Residue |
| A | HOH2790 |
| B | LYS510 |
| A | ARG462 |
| A | PHE464 |
| A | HIS465 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NA A 1540 |
| Chain | Residue |
| A | SER55 |
| A | LEU56 |
| A | GLU123 |
| A | ASP211 |
| A | HOH2701 |
| A | HOH2813 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT B 2519 |
| Chain | Residue |
| B | ALA245 |
| B | GLU249 |
| B | ILE268 |
| B | ALA271 |
| B | NAD2518 |
| B | HOH2979 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT B 2520 |
| Chain | Residue |
| A | LYS510 |
| B | ARG462 |
| B | PHE464 |
| B | HIS465 |
| B | HOH2749 |
| B | HOH2829 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NA B 2540 |
| Chain | Residue |
| B | SER55 |
| B | LEU56 |
| B | GLU123 |
| B | ASP211 |
| B | HOH2857 |
| B | HOH2872 |
| site_id | AC7 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE VAL A 1517 |
| Chain | Residue |
| A | GLU137 |
| A | PHE185 |
| A | CYS322 |
| A | SER323 |
| A | GLY477 |
| A | ALA478 |
| A | PHE485 |
| A | HOH2563 |
| A | HOH2660 |
| A | HOH3025 |
| A | HOH3026 |
| site_id | AC8 |
| Number of Residues | 35 |
| Details | BINDING SITE FOR RESIDUE NAD A 1518 |
| Chain | Residue |
| A | ILE180 |
| A | ALA181 |
| A | PRO182 |
| A | TRP183 |
| A | ASN184 |
| A | ILE189 |
| A | LYS207 |
| A | ALA209 |
| A | GLU210 |
| A | GLY240 |
| A | GLU241 |
| A | GLY244 |
| A | ALA245 |
| A | PHE258 |
| A | THR259 |
| A | GLY260 |
| A | SER261 |
| A | VAL264 |
| A | GLU288 |
| A | THR289 |
| A | GLY290 |
| A | CYS322 |
| A | GLU417 |
| A | PHE419 |
| A | LEU445 |
| A | PHE485 |
| A | ACT1519 |
| A | HOH2639 |
| A | HOH2828 |
| A | HOH2845 |
| A | HOH2862 |
| A | HOH3002 |
| A | HOH3031 |
| A | HOH3032 |
| A | HOH3034 |
| site_id | AC9 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE VAL B 2517 |
| Chain | Residue |
| B | GLU137 |
| B | PHE185 |
| B | SER323 |
| B | GLY477 |
| B | ALA478 |
| B | PHE485 |
| B | HOH2582 |
| B | HOH2664 |
| B | HOH3007 |
| B | HOH3008 |
| site_id | BC1 |
| Number of Residues | 32 |
| Details | BINDING SITE FOR RESIDUE NAD B 2518 |
| Chain | Residue |
| B | GLU210 |
| B | GLY240 |
| B | GLY244 |
| B | ALA245 |
| B | PHE258 |
| B | THR259 |
| B | GLY260 |
| B | SER261 |
| B | VAL264 |
| B | GLU288 |
| B | THR289 |
| B | GLY290 |
| B | CYS322 |
| B | GLU417 |
| B | PHE419 |
| B | LEU445 |
| B | PHE485 |
| B | ACT2519 |
| B | HOH2650 |
| B | HOH2653 |
| B | HOH2767 |
| B | HOH2768 |
| B | HOH2847 |
| B | HOH3006 |
| B | ILE180 |
| B | ALA181 |
| B | PRO182 |
| B | TRP183 |
| B | ASN184 |
| B | ILE189 |
| B | LYS207 |
| B | ALA209 |
| site_id | BC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE MPD B 1530 |
| Chain | Residue |
| A | GLU158 |
| B | PHE6 |
| B | TYR144 |
| B | ALA148 |
| B | HOH2604 |
| B | HOH2616 |
| B | HOH2655 |
| B | HOH2866 |
| site_id | BC3 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MPD A 1531 |
| Chain | Residue |
| A | ASP359 |
| A | HOH2607 |
| site_id | BC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MPD A 1532 |
| Chain | Residue |
| A | LEU275 |
| A | GLN279 |
| A | HOH2706 |
| A | HOH2999 |
| B | MPD2535 |
| site_id | BC5 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MPD A 1533 |
| Chain | Residue |
| A | TYR171 |
| B | HOH2810 |
| site_id | BC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MPD B 1534 |
| Chain | Residue |
| B | LEU275 |
| B | GLN279 |
| B | HOH2733 |
| B | HOH2913 |
| site_id | BC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MPD B 1535 |
| Chain | Residue |
| A | PHE170 |
| B | PRO94 |
| B | ARG462 |
| B | HOH2732 |
| B | HOH2997 |
| site_id | BC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MPD A 1536 |
| Chain | Residue |
| A | ARG36 |
| A | TYR38 |
| A | PRO39 |
| A | TRP46 |
| site_id | BC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MPD A 2530 |
| Chain | Residue |
| A | PHE6 |
| A | TYR144 |
| A | ALA148 |
| A | HOH2614 |
| A | HOH2622 |
| A | HOH2749 |
| B | GLU158 |
| site_id | CC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MPD B 2531 |
| Chain | Residue |
| B | ARG36 |
| B | GLU221 |
| site_id | CC2 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE MPD B 2532 |
| Chain | Residue |
| A | LEU27 |
| A | ARG28 |
| A | TYR122 |
| A | GLU355 |
| B | PRO353 |
| B | GLU355 |
| B | GLU356 |
| B | HOH2659 |
| B | HOH2898 |
| site_id | CC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MPD B 2533 |
| Chain | Residue |
| A | PRO94 |
| A | ARG462 |
| A | HOH2694 |
| A | HOH2848 |
| B | TYR154 |
| B | PHE170 |
| site_id | CC4 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE MPD B 2534 |
| Chain | Residue |
| B | ARG151 |
| site_id | CC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MPD B 2535 |
| Chain | Residue |
| A | MPD1532 |
| B | GLU416 |
| B | HOH2565 |
| B | HOH2722 |
| site_id | CC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MPD B 2536 |
| Chain | Residue |
| B | VAL312 |
| B | TYR315 |
| B | GLY316 |
| B | ASP359 |
| B | HOH2651 |
Functional Information from PROSITE/UniProt
| site_id | PS00070 |
| Number of Residues | 12 |
| Details | ALDEHYDE_DEHYDR_CYS Aldehyde dehydrogenases cysteine active site. YgFQGQKCSAAS |
| Chain | Residue | Details |
| A | TYR315-SER326 |
| site_id | PS00687 |
| Number of Residues | 8 |
| Details | ALDEHYDE_DEHYDR_GLU Aldehyde dehydrogenases glutamic acid active site. VETGGKDA |
| Chain | Residue | Details |
| A | VAL287-ALA294 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1a4s |
| Chain | Residue | Details |
| A | CYS322 | |
| A | ASN184 | |
| A | GLU288 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1a4s |
| Chain | Residue | Details |
| B | CYS322 | |
| B | ASN184 | |
| B | GLU288 |
