2E68
Crystal structure of Trypanosoma cruzi dihydroorotate dehydrogenase in complex with dihydroorotate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004152 | molecular_function | dihydroorotate dehydrogenase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| A | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| A | 0006222 | biological_process | UMP biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016627 | molecular_function | oxidoreductase activity, acting on the CH-CH group of donors |
| A | 0044205 | biological_process | 'de novo' UMP biosynthetic process |
| A | 1990663 | molecular_function | dihydroorotate dehydrogenase (fumarate) activity |
| B | 0004152 | molecular_function | dihydroorotate dehydrogenase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| B | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| B | 0006222 | biological_process | UMP biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016627 | molecular_function | oxidoreductase activity, acting on the CH-CH group of donors |
| B | 0044205 | biological_process | 'de novo' UMP biosynthetic process |
| B | 1990663 | molecular_function | dihydroorotate dehydrogenase (fumarate) activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NCO A 1352 |
| Chain | Residue |
| A | GLN275 |
| A | GLU276 |
| A | HOH1523 |
| B | GLN275 |
| B | GLU276 |
| B | GLY278 |
| site_id | AC2 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE FMN A 1350 |
| Chain | Residue |
| A | LYS43 |
| A | SER44 |
| A | ASN67 |
| A | ASN127 |
| A | LYS164 |
| A | VAL193 |
| A | ASN194 |
| A | GLY221 |
| A | GLY222 |
| A | ILE225 |
| A | CYS248 |
| A | GLY249 |
| A | GLY250 |
| A | GLY271 |
| A | THR272 |
| A | DOR1353 |
| A | HOH1366 |
| A | HOH1418 |
| A | HOH1425 |
| A | ALA18 |
| A | ALA19 |
| A | GLY20 |
| site_id | AC3 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE DOR A 1353 |
| Chain | Residue |
| A | LYS43 |
| A | ASN67 |
| A | MET69 |
| A | GLY70 |
| A | LEU71 |
| A | ASN127 |
| A | CYS130 |
| A | PRO131 |
| A | ASN132 |
| A | ASN194 |
| A | SER195 |
| A | FMN1350 |
| site_id | AC4 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE FMN B 2350 |
| Chain | Residue |
| B | ALA18 |
| B | ALA19 |
| B | GLY20 |
| B | LYS43 |
| B | SER44 |
| B | ASN67 |
| B | ASN127 |
| B | LYS164 |
| B | VAL193 |
| B | ASN194 |
| B | GLY221 |
| B | GLY222 |
| B | ILE225 |
| B | CYS248 |
| B | GLY249 |
| B | GLY250 |
| B | GLY271 |
| B | THR272 |
| B | DOR2353 |
| B | HOH2354 |
| B | HOH2360 |
| B | HOH2400 |
| site_id | AC5 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE DOR B 2353 |
| Chain | Residue |
| B | LYS43 |
| B | ASN67 |
| B | MET69 |
| B | GLY70 |
| B | LEU71 |
| B | ASN127 |
| B | CYS130 |
| B | PRO131 |
| B | ASN132 |
| B | ASN194 |
| B | SER195 |
| B | FMN2350 |
| site_id | AC6 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL A 1361 |
| Chain | Residue |
| A | CYS31 |
| A | ALA34 |
| A | SER35 |
| A | HOH1417 |
| A | HOH1449 |
| B | CYS31 |
| B | ALA34 |
| B | SER35 |
| site_id | AC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL A 1362 |
| Chain | Residue |
| A | ILE171 |
| A | ARG239 |
| A | HOH1495 |
| A | HOH1502 |
| A | HOH1596 |
| B | LYS214 |
| B | GLN215 |
| B | PHE217 |
| B | HOH2565 |
| site_id | AC8 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL A 1363 |
| Chain | Residue |
| B | ARG239 |
| A | LYS214 |
| A | GLN215 |
| A | PHE217 |
| A | HOH1434 |
| A | HOH1442 |
| A | HOH1488 |
| A | HOH1598 |
| B | ILE171 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 1365 |
| Chain | Residue |
| A | THR176 |
| B | LEU80 |
| B | ARG112 |
| B | HOH2396 |
| B | HOH2438 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Nucleophile => ECO:0000250 |
| Chain | Residue | Details |
| A | CYS130 | |
| B | CYS130 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:18302934 |
| Chain | Residue | Details |
| A | ALA19 | |
| B | GLY222 | |
| B | GLY249 | |
| B | GLY271 | |
| A | LYS164 | |
| A | VAL193 | |
| A | GLY222 | |
| A | GLY249 | |
| A | GLY271 | |
| B | ALA19 | |
| B | LYS164 | |
| B | VAL193 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 10 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | LYS43 | |
| B | ASN194 | |
| A | ASN67 | |
| A | ASN127 | |
| A | ASN132 | |
| A | ASN194 | |
| B | LYS43 | |
| B | ASN67 | |
| B | ASN127 | |
| B | ASN132 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 2dor |
| Chain | Residue | Details |
| A | LYS43 | |
| A | CYS130 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 2dor |
| Chain | Residue | Details |
| B | LYS43 | |
| B | CYS130 |
| site_id | CSA3 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 2dor |
| Chain | Residue | Details |
| A | CYS130 |
| site_id | CSA4 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 2dor |
| Chain | Residue | Details |
| B | CYS130 |
