2E01

Crystal structure of H369A mutant of yeast bleomycin hydrolase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
A	0003677	molecular_function	DNA binding
A	0003690	molecular_function	double-stranded DNA binding
A	0003697	molecular_function	single-stranded DNA binding
A	0003729	molecular_function	mRNA binding
A	0004177	molecular_function	aminopeptidase activity
A	0004197	molecular_function	cysteine-type endopeptidase activity
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0006508	biological_process	proteolysis
A	0008234	molecular_function	cysteine-type peptidase activity
A	0009636	biological_process	response to toxic substance
A	0043418	biological_process	homocysteine catabolic process
A	0046677	biological_process	response to antibiotic
A	0070005	molecular_function	cysteine-type aminopeptidase activity

Functional Information from PROSITE/UniProt

site_id	PS00139
Number of Residues	12
Details	THIOL_PROTEASE_CYS Eukaryotic thiol (cysteine) proteases cysteine active site. QKsSGRCWLfAA

Chain	Residue	Details
A	GLN67-ALA78

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE:

Chain	Residue	Details
A	LEU102
A	SER398
A	ASP421

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 624

Chain	Residue	Details
A	GLU96	electrostatic stabiliser
A	LEU102	electrostatic stabiliser, nucleofuge, nucleophile, proton acceptor, proton donor
A	SER398	proton acceptor, proton donor
A	ASP421	electrostatic stabiliser

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