2C3D
2.15 Angstrom crystal structure of 2-ketopropyl coenzyme M oxidoreductase carboxylase with a coenzyme M disulfide bound at the active site
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003955 | molecular_function | NAD(P)H dehydrogenase (quinone) activity |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0042208 | biological_process | propylene catabolic process |
| A | 0050628 | molecular_function | 2-oxopropyl-CoM reductase (carboxylating) activity |
| A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| B | 0003955 | molecular_function | NAD(P)H dehydrogenase (quinone) activity |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0042208 | biological_process | propylene catabolic process |
| B | 0050628 | molecular_function | 2-oxopropyl-CoM reductase (carboxylating) activity |
| B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 34 |
| Details | BINDING SITE FOR RESIDUE FAD A1524 |
| Chain | Residue |
| A | GLY50 |
| A | ALA86 |
| A | CYS87 |
| A | HIS90 |
| A | HIS91 |
| A | ALA158 |
| A | ALA181 |
| A | VAL182 |
| A | HIS202 |
| A | THR225 |
| A | GLU314 |
| A | GLY52 |
| A | GLY352 |
| A | ASP353 |
| A | MET359 |
| A | GLU360 |
| A | MET361 |
| A | ALA364 |
| A | PHE390 |
| A | HOH2080 |
| A | HOH2082 |
| A | HOH2141 |
| A | ALA53 |
| A | HOH2232 |
| A | HOH2323 |
| A | HOH2324 |
| B | PHE501 |
| B | HOH2309 |
| A | ALA54 |
| A | ASP73 |
| A | ARG74 |
| A | TRP75 |
| A | GLY80 |
| A | SER81 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE COM A1525 |
| Chain | Residue |
| A | ARG56 |
| A | PHE57 |
| A | GLY79 |
| A | MET140 |
| A | MET361 |
| A | ARG365 |
| B | COM1525 |
| B | HOH2328 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE COM A1526 |
| Chain | Residue |
| A | MET419 |
| A | LEU431 |
| A | PHE501 |
| A | HIS506 |
| A | GLN509 |
| A | HOH2325 |
| A | HOH2326 |
| B | MET140 |
| B | COM1526 |
| site_id | AC4 |
| Number of Residues | 34 |
| Details | BINDING SITE FOR RESIDUE FAD B1524 |
| Chain | Residue |
| A | PHE501 |
| A | HOH2313 |
| B | GLY50 |
| B | GLY52 |
| B | ALA53 |
| B | ALA54 |
| B | ASP73 |
| B | ARG74 |
| B | TRP75 |
| B | GLY80 |
| B | SER81 |
| B | ALA86 |
| B | CYS87 |
| B | HIS90 |
| B | HIS91 |
| B | ALA158 |
| B | ALA181 |
| B | VAL182 |
| B | GLY183 |
| B | HIS202 |
| B | THR225 |
| B | GLU314 |
| B | GLY352 |
| B | ASP353 |
| B | MET359 |
| B | GLU360 |
| B | MET361 |
| B | ALA364 |
| B | PHE390 |
| B | HOH2038 |
| B | HOH2212 |
| B | HOH2218 |
| B | HOH2325 |
| B | HOH2326 |
| site_id | AC5 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE COM B1525 |
| Chain | Residue |
| A | MET140 |
| A | COM1525 |
| B | MET419 |
| B | LEU431 |
| B | PHE501 |
| B | HIS506 |
| B | GLN509 |
| B | HOH2327 |
| B | HOH2328 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE COM B1526 |
| Chain | Residue |
| B | MET361 |
| B | ARG365 |
| A | COM1526 |
| A | HOH2325 |
| B | ARG56 |
| B | PHE57 |
| B | GLY79 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:12390015, ECO:0000269|PubMed:16388586, ECO:0000269|PubMed:21192936, ECO:0007744|PDB:1MO9, ECO:0007744|PDB:1MOK, ECO:0007744|PDB:2C3C, ECO:0007744|PDB:2C3D, ECO:0007744|PDB:3Q6J |
| Chain | Residue | Details |
| A | ALA158 | |
| A | ASP353 | |
| A | MET361 | |
| A | PHE501 | |
| B | ALA53 | |
| B | SER81 | |
| B | ALA158 | |
| B | ASP353 | |
| B | MET361 | |
| B | PHE501 | |
| A | ALA53 | |
| A | SER81 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:12390015, ECO:0000269|PubMed:21192936, ECO:0000305|PubMed:16388586, ECO:0007744|PDB:1MO9, ECO:0007744|PDB:3Q6J |
| Chain | Residue | Details |
| A | ARG56 | |
| A | ARG365 | |
| B | ARG56 | |
| B | ARG365 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:21192936, ECO:0000305|PubMed:16388586, ECO:0007744|PDB:3Q6J |
| Chain | Residue | Details |
| A | CYS82 | |
| B | CYS82 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:16388586, ECO:0000269|PubMed:21192936, ECO:0007744|PDB:2C3C, ECO:0007744|PDB:3Q6J |
| Chain | Residue | Details |
| A | GLY222 | |
| A | ARG245 | |
| A | GLU360 | |
| B | GLY222 | |
| B | ARG245 | |
| B | GLU360 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 378 |
| Chain | Residue | Details |
| A | LEU78 | electrostatic stabiliser, modifies pKa |
| A | CYS82 | covalent catalysis |
| A | CYS87 | covalent catalysis |
| A | HIS137 | modifies pKa |
| A | PHE501 | electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 378 |
| Chain | Residue | Details |
| B | LEU78 | electrostatic stabiliser, modifies pKa |
| B | CYS82 | covalent catalysis |
| B | CYS87 | covalent catalysis |
| B | HIS137 | modifies pKa |
| B | PHE501 | electrostatic stabiliser |
