2C2L

Crystal structure of the CHIP U-box E3 ubiquitin ligase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000151	cellular_component	ubiquitin ligase complex
A	0000165	biological_process	MAPK cascade
A	0000209	biological_process	protein polyubiquitination
A	0001664	molecular_function	G protein-coupled receptor binding
A	0002931	biological_process	response to ischemia
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005783	cellular_component	endoplasmic reticulum
A	0005829	cellular_component	cytosol
A	0006281	biological_process	DNA repair
A	0006457	biological_process	protein folding
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0006513	biological_process	protein monoubiquitination
A	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
A	0006974	biological_process	DNA damage response
A	0007165	biological_process	signal transduction
A	0009893	biological_process	positive regulation of metabolic process
A	0010614	biological_process	negative regulation of cardiac muscle hypertrophy
A	0016567	biological_process	protein ubiquitination
A	0016740	molecular_function	transferase activity
A	0019899	molecular_function	enzyme binding
A	0019900	molecular_function	kinase binding
A	0030018	cellular_component	Z disc
A	0030163	biological_process	protein catabolic process
A	0030512	biological_process	negative regulation of transforming growth factor beta receptor signaling pathway
A	0030544	molecular_function	Hsp70 protein binding
A	0030674	molecular_function	protein-macromolecule adaptor activity
A	0030911	molecular_function	TPR domain binding
A	0030968	biological_process	endoplasmic reticulum unfolded protein response
A	0031072	molecular_function	heat shock protein binding
A	0031371	cellular_component	ubiquitin conjugating enzyme complex
A	0031398	biological_process	positive regulation of protein ubiquitination
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0031647	biological_process	regulation of protein stability
A	0031943	biological_process	regulation of glucocorticoid metabolic process
A	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
A	0033554	biological_process	cellular response to stress
A	0034392	biological_process	negative regulation of smooth muscle cell apoptotic process
A	0034393	biological_process	positive regulation of smooth muscle cell apoptotic process
A	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
A	0034605	biological_process	cellular response to heat
A	0035359	biological_process	negative regulation of peroxisome proliferator activated receptor signaling pathway
A	0036503	biological_process	ERAD pathway
A	0042405	cellular_component	nuclear inclusion body
A	0042803	molecular_function	protein homodimerization activity
A	0043066	biological_process	negative regulation of apoptotic process
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0045862	biological_process	positive regulation of proteolysis
A	0046332	molecular_function	SMAD binding
A	0050821	biological_process	protein stabilization
A	0051087	molecular_function	protein-folding chaperone binding
A	0051787	molecular_function	misfolded protein binding
A	0051865	biological_process	protein autoubiquitination
A	0051879	molecular_function	Hsp90 protein binding
A	0061630	molecular_function	ubiquitin protein ligase activity
A	0061684	biological_process	chaperone-mediated autophagy
A	0070412	molecular_function	R-SMAD binding
A	0070534	biological_process	protein K63-linked ubiquitination
A	0071218	biological_process	cellular response to misfolded protein
A	0071456	biological_process	cellular response to hypoxia
A	0090035	biological_process	positive regulation of chaperone-mediated protein complex assembly
A	0101031	cellular_component	protein folding chaperone complex
A	1901526	biological_process	positive regulation of mitophagy
A	1904294	biological_process	positive regulation of ERAD pathway
A	1904694	biological_process	negative regulation of vascular associated smooth muscle contraction
B	0000151	cellular_component	ubiquitin ligase complex
B	0000165	biological_process	MAPK cascade
B	0000209	biological_process	protein polyubiquitination
B	0001664	molecular_function	G protein-coupled receptor binding
B	0002931	biological_process	response to ischemia
B	0004842	molecular_function	ubiquitin-protein transferase activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005783	cellular_component	endoplasmic reticulum
B	0005829	cellular_component	cytosol
B	0006281	biological_process	DNA repair
B	0006457	biological_process	protein folding
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0006513	biological_process	protein monoubiquitination
B	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
B	0006974	biological_process	DNA damage response
B	0007165	biological_process	signal transduction
B	0009893	biological_process	positive regulation of metabolic process
B	0010614	biological_process	negative regulation of cardiac muscle hypertrophy
B	0016567	biological_process	protein ubiquitination
B	0016740	molecular_function	transferase activity
B	0019899	molecular_function	enzyme binding
B	0019900	molecular_function	kinase binding
B	0030018	cellular_component	Z disc
B	0030163	biological_process	protein catabolic process
B	0030512	biological_process	negative regulation of transforming growth factor beta receptor signaling pathway
B	0030544	molecular_function	Hsp70 protein binding
B	0030674	molecular_function	protein-macromolecule adaptor activity
B	0030911	molecular_function	TPR domain binding
B	0030968	biological_process	endoplasmic reticulum unfolded protein response
B	0031072	molecular_function	heat shock protein binding
B	0031371	cellular_component	ubiquitin conjugating enzyme complex
B	0031398	biological_process	positive regulation of protein ubiquitination
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0031647	biological_process	regulation of protein stability
B	0031943	biological_process	regulation of glucocorticoid metabolic process
B	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
B	0033554	biological_process	cellular response to stress
B	0034392	biological_process	negative regulation of smooth muscle cell apoptotic process
B	0034393	biological_process	positive regulation of smooth muscle cell apoptotic process
B	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
B	0034605	biological_process	cellular response to heat
B	0035359	biological_process	negative regulation of peroxisome proliferator activated receptor signaling pathway
B	0036503	biological_process	ERAD pathway
B	0042405	cellular_component	nuclear inclusion body
B	0042803	molecular_function	protein homodimerization activity
B	0043066	biological_process	negative regulation of apoptotic process
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0045862	biological_process	positive regulation of proteolysis
B	0046332	molecular_function	SMAD binding
B	0050821	biological_process	protein stabilization
B	0051087	molecular_function	protein-folding chaperone binding
B	0051787	molecular_function	misfolded protein binding
B	0051865	biological_process	protein autoubiquitination
B	0051879	molecular_function	Hsp90 protein binding
B	0061630	molecular_function	ubiquitin protein ligase activity
B	0061684	biological_process	chaperone-mediated autophagy
B	0070412	molecular_function	R-SMAD binding
B	0070534	biological_process	protein K63-linked ubiquitination
B	0071218	biological_process	cellular response to misfolded protein
B	0071456	biological_process	cellular response to hypoxia
B	0090035	biological_process	positive regulation of chaperone-mediated protein complex assembly
B	0101031	cellular_component	protein folding chaperone complex
B	1901526	biological_process	positive regulation of mitophagy
B	1904294	biological_process	positive regulation of ERAD pathway
B	1904694	biological_process	negative regulation of vascular associated smooth muscle contraction
C	0000151	cellular_component	ubiquitin ligase complex
C	0000165	biological_process	MAPK cascade
C	0000209	biological_process	protein polyubiquitination
C	0001664	molecular_function	G protein-coupled receptor binding
C	0002931	biological_process	response to ischemia
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005783	cellular_component	endoplasmic reticulum
C	0005829	cellular_component	cytosol
C	0006281	biological_process	DNA repair
C	0006457	biological_process	protein folding
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0006513	biological_process	protein monoubiquitination
C	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
C	0006974	biological_process	DNA damage response
C	0007165	biological_process	signal transduction
C	0009893	biological_process	positive regulation of metabolic process
C	0010614	biological_process	negative regulation of cardiac muscle hypertrophy
C	0016567	biological_process	protein ubiquitination
C	0016740	molecular_function	transferase activity
C	0019899	molecular_function	enzyme binding
C	0019900	molecular_function	kinase binding
C	0030018	cellular_component	Z disc
C	0030163	biological_process	protein catabolic process
C	0030512	biological_process	negative regulation of transforming growth factor beta receptor signaling pathway
C	0030544	molecular_function	Hsp70 protein binding
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0030911	molecular_function	TPR domain binding
C	0030968	biological_process	endoplasmic reticulum unfolded protein response
C	0031072	molecular_function	heat shock protein binding
C	0031371	cellular_component	ubiquitin conjugating enzyme complex
C	0031398	biological_process	positive regulation of protein ubiquitination
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0031647	biological_process	regulation of protein stability
C	0031943	biological_process	regulation of glucocorticoid metabolic process
C	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
C	0033554	biological_process	cellular response to stress
C	0034392	biological_process	negative regulation of smooth muscle cell apoptotic process
C	0034393	biological_process	positive regulation of smooth muscle cell apoptotic process
C	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
C	0034605	biological_process	cellular response to heat
C	0035359	biological_process	negative regulation of peroxisome proliferator activated receptor signaling pathway
C	0036503	biological_process	ERAD pathway
C	0042405	cellular_component	nuclear inclusion body
C	0042803	molecular_function	protein homodimerization activity
C	0043066	biological_process	negative regulation of apoptotic process
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0045862	biological_process	positive regulation of proteolysis
C	0046332	molecular_function	SMAD binding
C	0050821	biological_process	protein stabilization
C	0051087	molecular_function	protein-folding chaperone binding
C	0051787	molecular_function	misfolded protein binding
C	0051865	biological_process	protein autoubiquitination
C	0051879	molecular_function	Hsp90 protein binding
C	0061630	molecular_function	ubiquitin protein ligase activity
C	0061684	biological_process	chaperone-mediated autophagy
C	0070412	molecular_function	R-SMAD binding
C	0070534	biological_process	protein K63-linked ubiquitination
C	0071218	biological_process	cellular response to misfolded protein
C	0071456	biological_process	cellular response to hypoxia
C	0090035	biological_process	positive regulation of chaperone-mediated protein complex assembly
C	0101031	cellular_component	protein folding chaperone complex
C	1901526	biological_process	positive regulation of mitophagy
C	1904294	biological_process	positive regulation of ERAD pathway
C	1904694	biological_process	negative regulation of vascular associated smooth muscle contraction
D	0000151	cellular_component	ubiquitin ligase complex
D	0000165	biological_process	MAPK cascade
D	0000209	biological_process	protein polyubiquitination
D	0001664	molecular_function	G protein-coupled receptor binding
D	0002931	biological_process	response to ischemia
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005783	cellular_component	endoplasmic reticulum
D	0005829	cellular_component	cytosol
D	0006281	biological_process	DNA repair
D	0006457	biological_process	protein folding
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0006513	biological_process	protein monoubiquitination
D	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
D	0006974	biological_process	DNA damage response
D	0007165	biological_process	signal transduction
D	0009893	biological_process	positive regulation of metabolic process
D	0010614	biological_process	negative regulation of cardiac muscle hypertrophy
D	0016567	biological_process	protein ubiquitination
D	0016740	molecular_function	transferase activity
D	0019899	molecular_function	enzyme binding
D	0019900	molecular_function	kinase binding
D	0030018	cellular_component	Z disc
D	0030163	biological_process	protein catabolic process
D	0030512	biological_process	negative regulation of transforming growth factor beta receptor signaling pathway
D	0030544	molecular_function	Hsp70 protein binding
D	0030674	molecular_function	protein-macromolecule adaptor activity
D	0030911	molecular_function	TPR domain binding
D	0030968	biological_process	endoplasmic reticulum unfolded protein response
D	0031072	molecular_function	heat shock protein binding
D	0031371	cellular_component	ubiquitin conjugating enzyme complex
D	0031398	biological_process	positive regulation of protein ubiquitination
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0031647	biological_process	regulation of protein stability
D	0031943	biological_process	regulation of glucocorticoid metabolic process
D	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
D	0033554	biological_process	cellular response to stress
D	0034392	biological_process	negative regulation of smooth muscle cell apoptotic process
D	0034393	biological_process	positive regulation of smooth muscle cell apoptotic process
D	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
D	0034605	biological_process	cellular response to heat
D	0035359	biological_process	negative regulation of peroxisome proliferator activated receptor signaling pathway
D	0036503	biological_process	ERAD pathway
D	0042405	cellular_component	nuclear inclusion body
D	0042803	molecular_function	protein homodimerization activity
D	0043066	biological_process	negative regulation of apoptotic process
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0045862	biological_process	positive regulation of proteolysis
D	0046332	molecular_function	SMAD binding
D	0050821	biological_process	protein stabilization
D	0051087	molecular_function	protein-folding chaperone binding
D	0051787	molecular_function	misfolded protein binding
D	0051865	biological_process	protein autoubiquitination
D	0051879	molecular_function	Hsp90 protein binding
D	0061630	molecular_function	ubiquitin protein ligase activity
D	0061684	biological_process	chaperone-mediated autophagy
D	0070412	molecular_function	R-SMAD binding
D	0070534	biological_process	protein K63-linked ubiquitination
D	0071218	biological_process	cellular response to misfolded protein
D	0071456	biological_process	cellular response to hypoxia
D	0090035	biological_process	positive regulation of chaperone-mediated protein complex assembly
D	0101031	cellular_component	protein folding chaperone complex
D	1901526	biological_process	positive regulation of mitophagy
D	1904294	biological_process	positive regulation of ERAD pathway
D	1904694	biological_process	negative regulation of vascular associated smooth muscle contraction

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SO4 A 1305

Chain	Residue
A	SER26
A	ALA27
A	ARG56
A	ASN57
D	SER26
D	ALA27
D	ARG56
D	ASN57

---

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 1306

Chain	Residue
A	GLN105
A	ASN117
A	ARG85

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 1307

Chain	Residue
A	GLN33
A	ARG36
C	LEU285
D	LYS235

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 B 1305

Chain	Residue
B	SER26
B	ALA27
B	ARG56
B	ASN57
C	ALA27
C	ARG56
C	ASN57

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 1306

Chain	Residue
B	ARG85
B	GLN105
B	GLU113
B	ARG120

---

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 1305

Chain	Residue
C	ARG85
C	GLN105
C	ASN117

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 1305

Chain	Residue
D	ARG85
D	GLN105
D	GLU113
D	ASN117

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	132
Details	Repeat: {"description":"TPR 1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	132
Details	Repeat: {"description":"TPR 2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	128
Details	Repeat: {"description":"TPR 3"}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	296
Details	Domain: {"description":"U-box"}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	396
Details	Region: {"description":"Required for interaction with MAPK7","evidences":[{"source":"UniProtKB","id":"Q9UNE7","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	216
Details	Region: {"description":"Required for interaction with and ubiquitination of MYOCD","evidences":[{"source":"UniProtKB","id":"A6HD62","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	640
Details	Region: {"description":"Required for ubiquitination of FOXO1","evidences":[{"source":"UniProtKB","id":"Q9UNE7","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	216
Details	Region: {"description":"Required for interaction with FOXO1","evidences":[{"source":"UniProtKB","id":"Q9UNE7","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19131326","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"21183079","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19131326","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q9UNE7","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	12
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"Q9UNE7","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	16
Details	Region: {"description":"Essential for interaction with SMYD3, TSC1 and STIP1/HOP","evidences":[{"source":"PubMed","id":"25738358","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29127155","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	12
Details	Region: {"description":"Essential for interaction with SGTA and TTC1","evidences":[{"source":"PubMed","id":"15708368","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---