2BOV
Molecular recognition of an ADP-ribosylating Clostridium botulinum C3 exoenzyme by RalA GTPase
Replaces: 1WCAFunctional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0001843 | biological_process | neural tube closure |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0003925 | molecular_function | G protein activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005525 | molecular_function | GTP binding |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005925 | cellular_component | focal adhesion |
| A | 0006887 | biological_process | exocytosis |
| A | 0006935 | biological_process | chemotaxis |
| A | 0007165 | biological_process | signal transduction |
| A | 0007265 | biological_process | Ras protein signal transduction |
| A | 0009986 | cellular_component | cell surface |
| A | 0016020 | cellular_component | membrane |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0017022 | molecular_function | myosin binding |
| A | 0017157 | biological_process | regulation of exocytosis |
| A | 0019003 | molecular_function | GDP binding |
| A | 0030139 | cellular_component | endocytic vesicle |
| A | 0030659 | cellular_component | cytoplasmic vesicle membrane |
| A | 0031623 | biological_process | receptor internalization |
| A | 0031625 | molecular_function | ubiquitin protein ligase binding |
| A | 0031755 | molecular_function | Edg-2 lysophosphatidic acid receptor binding |
| A | 0032154 | cellular_component | cleavage furrow |
| A | 0032956 | biological_process | regulation of actin cytoskeleton organization |
| A | 0045742 | biological_process | positive regulation of epidermal growth factor receptor signaling pathway |
| A | 0051117 | molecular_function | ATPase binding |
| A | 0051301 | biological_process | cell division |
| A | 0051491 | biological_process | positive regulation of filopodium assembly |
| A | 0051665 | biological_process | membrane raft localization |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0072655 | biological_process | establishment of protein localization to mitochondrion |
| A | 0090141 | biological_process | positive regulation of mitochondrial fission |
| A | 0090543 | cellular_component | Flemming body |
| A | 0097060 | cellular_component | synaptic membrane |
| A | 0098685 | cellular_component | Schaffer collateral - CA1 synapse |
| A | 0099149 | biological_process | regulation of postsynaptic neurotransmitter receptor internalization |
| B | 0005576 | cellular_component | extracellular region |
| B | 0016763 | molecular_function | pentosyltransferase activity |
| B | 1990404 | molecular_function | NAD+-protein mono-ADP-ribosyltransferase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG A1185 |
| Chain | Residue |
| A | SER28 |
| A | GDP1184 |
| A | HOH2029 |
| A | HOH2038 |
| A | HOH2056 |
| site_id | AC2 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE GDP A1184 |
| Chain | Residue |
| A | SER28 |
| A | ALA29 |
| A | PHE39 |
| A | GLU41 |
| A | ASP42 |
| A | ASN127 |
| A | LYS128 |
| A | ASP130 |
| A | LEU131 |
| A | SER157 |
| A | ALA158 |
| A | LYS159 |
| A | MG1185 |
| A | HOH2029 |
| A | HOH2128 |
| A | HOH2129 |
| A | HOH2130 |
| A | HOH2131 |
| A | GLY24 |
| A | VAL25 |
| A | GLY26 |
| A | LYS27 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | Motif: {"description":"Effector region"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 14 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"15809419","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16177825","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"(Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL","evidences":[{"source":"PubMed","id":"8858106","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 199 |
| Details | Domain: {"description":"TR mART core","evidences":[{"source":"PROSITE-ProRule","id":"PRU01340","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 3 |
| Details | Active site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01340","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 13 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"12029083","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16177825","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Site: {"description":"Transition state stabilizer"} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| A | GLN72 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| B | GLU1214 |
| site_id | MCSA1 |
| Number of Residues | 2 |
| Details | M-CSA 824 |
| Chain | Residue | Details |
| B | SER1174 | electrostatic stabiliser, polar interaction |
| B | GLU1214 | electrostatic stabiliser, polar interaction, proton acceptor, proton donor |
