2BHP
Crystal Analysis of 1-Pyrroline-5-Carboxylate Dehydrogenase from Thermus with bound NAD.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0003842 | molecular_function | L-glutamate gamma-semialdehyde dehydrogenase activity |
| A | 0004657 | molecular_function | proline dehydrogenase activity |
| A | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| A | 0010133 | biological_process | L-proline catabolic process to L-glutamate |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0003842 | molecular_function | L-glutamate gamma-semialdehyde dehydrogenase activity |
| B | 0004657 | molecular_function | proline dehydrogenase activity |
| B | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| B | 0010133 | biological_process | L-proline catabolic process to L-glutamate |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT A1518 |
| Chain | Residue |
| A | ALA245 |
| A | GLU249 |
| A | ILE268 |
| A | ALA271 |
| A | NAD1517 |
| A | HOH2604 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT A1519 |
| Chain | Residue |
| A | HOH2605 |
| A | HOH2606 |
| B | LYS510 |
| A | ARG462 |
| A | PHE464 |
| A | HIS465 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE ACT A1520 |
| Chain | Residue |
| A | ARG20 |
| A | ASN357 |
| A | HOH2444 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NA A1526 |
| Chain | Residue |
| A | SER55 |
| A | LEU56 |
| A | GLU123 |
| A | ASP211 |
| A | HOH2150 |
| A | HOH2449 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT B1520 |
| Chain | Residue |
| B | ALA245 |
| B | GLU249 |
| B | ILE268 |
| B | ALA271 |
| B | NAD1517 |
| B | HOH2581 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT B1521 |
| Chain | Residue |
| A | LYS510 |
| B | ARG462 |
| B | PHE464 |
| B | HIS465 |
| B | HOH2582 |
| B | HOH2583 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NA B1524 |
| Chain | Residue |
| B | SER55 |
| B | LEU56 |
| B | GLU123 |
| B | ASP211 |
| B | HOH2143 |
| B | HOH2436 |
| site_id | AC8 |
| Number of Residues | 32 |
| Details | BINDING SITE FOR RESIDUE NAD A1517 |
| Chain | Residue |
| A | ILE180 |
| A | ALA181 |
| A | PRO182 |
| A | TRP183 |
| A | ASN184 |
| A | ILE189 |
| A | LYS207 |
| A | ALA209 |
| A | GLU210 |
| A | GLY240 |
| A | GLY244 |
| A | ALA245 |
| A | PHE258 |
| A | THR259 |
| A | GLY260 |
| A | SER261 |
| A | VAL264 |
| A | GLU288 |
| A | THR289 |
| A | GLY290 |
| A | CYS322 |
| A | GLU417 |
| A | PHE419 |
| A | LEU445 |
| A | PHE485 |
| A | ACT1518 |
| A | HOH2323 |
| A | HOH2345 |
| A | HOH2600 |
| A | HOH2601 |
| A | HOH2602 |
| A | HOH2603 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MRD A1521 |
| Chain | Residue |
| A | GLN279 |
| A | HOH2607 |
| A | HOH2608 |
| site_id | BC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MRD A1523 |
| Chain | Residue |
| A | VAL312 |
| A | ASP359 |
| A | HOH2259 |
| A | HOH2610 |
| site_id | BC2 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE MRD A1527 |
| Chain | Residue |
| B | TYR171 |
| site_id | BC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MRD A1528 |
| Chain | Residue |
| A | TYR144 |
| A | ALA148 |
| A | LEU500 |
| A | HOH2281 |
| A | HOH2612 |
| A | HOH2613 |
| B | GLU158 |
| site_id | BC4 |
| Number of Residues | 34 |
| Details | BINDING SITE FOR RESIDUE NAD B1517 |
| Chain | Residue |
| B | THR259 |
| B | GLY260 |
| B | SER261 |
| B | VAL264 |
| B | GLU288 |
| B | THR289 |
| B | GLY290 |
| B | CYS322 |
| B | GLU417 |
| B | PHE419 |
| B | LEU445 |
| B | PHE485 |
| B | ACT1520 |
| B | HOH2311 |
| B | HOH2315 |
| B | HOH2573 |
| B | HOH2574 |
| B | HOH2575 |
| B | HOH2576 |
| B | HOH2577 |
| B | HOH2578 |
| B | ILE180 |
| B | ALA181 |
| B | PRO182 |
| B | TRP183 |
| B | ASN184 |
| B | ILE189 |
| B | LYS207 |
| B | ALA209 |
| B | GLU210 |
| B | GLY240 |
| B | GLY244 |
| B | ALA245 |
| B | PHE258 |
| site_id | BC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MRD B1523 |
| Chain | Residue |
| A | ARG28 |
| A | TYR122 |
| B | PRO353 |
| B | GLU355 |
| B | GLU356 |
| B | HOH2584 |
| B | HOH2585 |
| site_id | BC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MRD B1527 |
| Chain | Residue |
| B | GLN279 |
| B | HOH2586 |
| B | HOH2587 |
| site_id | BC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MPD A1522 |
| Chain | Residue |
| A | ARG36 |
| A | TRP46 |
| A | GLU221 |
| A | HOH2111 |
| site_id | BC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MPD A1524 |
| Chain | Residue |
| A | TYR375 |
| A | ILE378 |
| A | ARG412 |
| A | HOH2457 |
| A | HOH2461 |
| site_id | BC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MPD A1525 |
| Chain | Residue |
| A | ARG151 |
| A | TYR171 |
| A | HOH2289 |
| A | HOH2611 |
| site_id | CC1 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE MPD B1518 |
| Chain | Residue |
| A | GLU158 |
| A | HOH2303 |
| B | PHE6 |
| B | TYR144 |
| B | ARG147 |
| B | ALA148 |
| B | HOH2262 |
| B | HOH2579 |
| B | HOH2580 |
Functional Information from PROSITE/UniProt
| site_id | PS00070 |
| Number of Residues | 12 |
| Details | ALDEHYDE_DEHYDR_CYS Aldehyde dehydrogenases cysteine active site. YgFQGQKCSAAS |
| Chain | Residue | Details |
| A | TYR315-SER326 |
| site_id | PS00687 |
| Number of Residues | 8 |
| Details | ALDEHYDE_DEHYDR_GLU Aldehyde dehydrogenases glutamic acid active site. VETGGKDA |
| Chain | Residue | Details |
| A | VAL287-ALA294 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1a4s |
| Chain | Residue | Details |
| A | CYS322 | |
| A | ASN184 | |
| A | GLU288 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1a4s |
| Chain | Residue | Details |
| B | CYS322 | |
| B | ASN184 | |
| B | GLU288 |
