2B6O

Electron crystallographic structure of lens Aquaporin-0 (AQP0) (lens MIP) at 1.9A resolution, in a closed pore state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005212	molecular_function	structural constituent of eye lens
A	0005516	molecular_function	calmodulin binding
A	0005886	cellular_component	plasma membrane
A	0006833	biological_process	water transport
A	0015250	molecular_function	water channel activity
A	0015267	molecular_function	channel activity
A	0016020	cellular_component	membrane
A	0016324	cellular_component	apical plasma membrane
A	0034109	biological_process	homotypic cell-cell adhesion
A	0036438	biological_process	maintenance of lens transparency
A	0055085	biological_process	transmembrane transport
A	0070161	cellular_component	anchoring junction
A	0098631	molecular_function	cell adhesion mediator activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MC3 A 264

Chain	Residue
A	ARG196
A	MC3269
A	MC3270
A	MC3272
A	HOH273
A	HOH304

---

site_id	AC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE MC3 A 265

Chain	Residue
A	SER106
A	MC3270
A	HOH291
A	HOH301
A	HOH327
A	ALA102
A	VAL103
A	TYR105

---

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE MC3 A 266

Chain	Residue
A	LEU83
A	LEU84
A	ILE87
A	VAL90
A	VAL91
A	LEU94
A	LYS238
A	MC3267
A	MC3271

---

site_id	AC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE MC3 A 267

Chain	Residue
A	ARG5
A	SER6
A	PHE9
A	TRP10
A	LEU84
A	CYS88
A	MC3266
A	MC3272

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MC3 A 268

Chain	Residue
A	ALA7
A	TRP10
A	ARG11
A	PHE14

---

site_id	AC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MC3 A 269

Chain	Residue
A	MC3264
A	MC3272

---

site_id	AC7
Number of Residues	8
Details	BINDING SITE FOR RESIDUE MC3 A 270

Chain	Residue
A	LEU95
A	TYR105
A	ILE193
A	LEU194
A	ARG196
A	MC3264
A	MC3265
A	HOH326

---

site_id	AC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MC3 A 271

Chain	Residue
A	MC3266

---

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MC3 A 272

Chain	Residue
A	MC3264
A	MC3267
A	MC3269

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	29
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. IGAGLGSLLYdFllfprlksvserl.....SILK

Chain	Residue	Details
A	ILE210-LYS238

---

site_id	PS00221
Number of Residues	9
Details	MIP MIP family signature. HVNPAVTFA

Chain	Residue	Details
A	HIS66-ALA74

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	17
Details	Transmembrane: {"description":"Helical; Name=1","evidences":[{"source":"PubMed","id":"16319884","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2B6O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	35
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"16319884","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	18
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"16319884","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2B6O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	20
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"16319884","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	27
Details	Intramembrane: {"description":"Discontinuously helical","evidences":[{"source":"PubMed","id":"16319884","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2B6O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"16319884","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2B6O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	20
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"16319884","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2B6O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	17
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"16319884","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2B6O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	17
Details	Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"PubMed","id":"16319884","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2B6O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	10
Details	Region: {"description":"Interaction with CALM","evidences":[{"source":"UniProtKB","id":"P06624","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Motif: {"description":"NPA 1","evidences":[{"source":"PubMed","id":"16319884","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Motif: {"description":"NPA 2","evidences":[{"source":"PubMed","id":"16319884","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	1
Details	Site: {"description":"Important for water channel gating","evidences":[{"source":"UniProtKB","id":"P06624","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	1
Details	Site: {"description":"Cleavage; promotes interactions between tetramers from adjoining membranes","evidences":[{"source":"PubMed","id":"15351655","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P06624","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---