2AKI
Normal mode-based flexible fitted coordinates of a translocating SecYEG protein-conducting channel into the cryo-EM map of a SecYEG-nascent chain-70S ribosome complex from E. coli
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0009306 | biological_process | protein secretion |
| A | 0015450 | molecular_function | protein-transporting ATPase activity |
| A | 0016020 | cellular_component | membrane |
| B | 0005048 | molecular_function | signal sequence binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006605 | biological_process | protein targeting |
| B | 0006616 | biological_process | SRP-dependent cotranslational protein targeting to membrane, translocation |
| B | 0006886 | biological_process | intracellular protein transport |
| B | 0008320 | molecular_function | protein transmembrane transporter activity |
| B | 0015031 | biological_process | protein transport |
| B | 0016020 | cellular_component | membrane |
| B | 0031522 | cellular_component | cell envelope Sec protein transport complex |
| B | 0032978 | biological_process | protein insertion into membrane from inner side |
| B | 0043952 | biological_process | protein transport by the Sec complex |
| B | 0065002 | biological_process | intracellular protein transmembrane transport |
| C | 0006605 | biological_process | protein targeting |
| C | 0006886 | biological_process | intracellular protein transport |
| C | 0008320 | molecular_function | protein transmembrane transporter activity |
| C | 0009306 | biological_process | protein secretion |
| C | 0016020 | cellular_component | membrane |
| X | 0009306 | biological_process | protein secretion |
| X | 0015450 | molecular_function | protein-transporting ATPase activity |
| X | 0016020 | cellular_component | membrane |
| Y | 0005048 | molecular_function | signal sequence binding |
| Y | 0005515 | molecular_function | protein binding |
| Y | 0005886 | cellular_component | plasma membrane |
| Y | 0006605 | biological_process | protein targeting |
| Y | 0006616 | biological_process | SRP-dependent cotranslational protein targeting to membrane, translocation |
| Y | 0006886 | biological_process | intracellular protein transport |
| Y | 0008320 | molecular_function | protein transmembrane transporter activity |
| Y | 0015031 | biological_process | protein transport |
| Y | 0016020 | cellular_component | membrane |
| Y | 0031522 | cellular_component | cell envelope Sec protein transport complex |
| Y | 0032978 | biological_process | protein insertion into membrane from inner side |
| Y | 0043952 | biological_process | protein transport by the Sec complex |
| Y | 0065002 | biological_process | intracellular protein transmembrane transport |
| Z | 0006605 | biological_process | protein targeting |
| Z | 0006886 | biological_process | intracellular protein transport |
| Z | 0008320 | molecular_function | protein transmembrane transporter activity |
| Z | 0009306 | biological_process | protein secretion |
| Z | 0016020 | cellular_component | membrane |
Functional Information from PROSITE/UniProt
| site_id | PS00755 |
| Number of Residues | 20 |
| Details | SECY_1 Protein secY signature 1. SIFaLGImPYIsASIIIQLL |
| Chain | Residue | Details |
| Y | SER76-LEU95 |
| site_id | PS00756 |
| Number of Residues | 18 |
| Details | SECY_2 Protein secY signature 2. WLgEqITer.GIGNGiSII |
| Chain | Residue | Details |
| Y | TRP173-ILE190 |
| site_id | PS01067 |
| Number of Residues | 29 |
| Details | SECE_SEC61G Protein secE/sec61-gamma signature. FaREaRteVrKviWPtrqEtlhttLIVAA |
| Chain | Residue | Details |
| Z | PHE71-ALA99 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 80 |
| Details | TRANSMEM: Helical => ECO:0000305 |
| Chain | Residue | Details |
| B | LEU24-LEU80 | |
| B | ILE77-VAL97 | |
| B | LEU125-MET145 | |
| B | PHE154-LEU174 | |
| B | ILE183-ILE203 | |
| B | PHE217-VAL237 | |
| B | VAL274-PHE294 | |
| B | TYR317-PHE337 | |
| B | MET370-PHE390 | |
| B | VAL397-MET417 | |
| C | ALA98-LEU115 | |
| Y | TYR317-PHE337 | |
| Y | MET370-PHE390 | |
| Y | VAL397-MET417 | |
| Z | VAL20-GLY32 | |
| Z | VAL49-LEU60 | |
| Z | ALA98-LEU115 | |
| C | VAL20-GLY32 | |
| C | VAL49-LEU60 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 52 |
| Details | TOPO_DOM: Periplasmic => ECO:0000305|PubMed:2050112 |
| Chain | Residue | Details |
| Y | PHE330-TYR380 | |
| B | MET1-ARG22 | |
| B | VAL97-LYS115 | |
| B | THR179-ILE183 | |
| B | GLU238-GLY273 | |
| B | PHE330-TYR380 | |
| Z | ASN33-VAL48 | |
| Z | VAL116-PHE127 | |
| C | ASN33-VAL48 | |
| C | VAL116-PHE127 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 72 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000305|PubMed:2050112 |
| Chain | Residue | Details |
| Z | LEU61-VAL97 | |
| C | LEU61-VAL97 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 48 |
| Details | TOPO_DOM: Periplasmic => ECO:0000250 |
| Chain | Residue | Details |
| Y | GLY36-THR96 | |
| B | GLY36-THR96 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 70 |
| Details | TRANSMEM: Discontinuously helical; Name=2 => ECO:0000250 |
| Chain | Residue | Details |
| Y | VAL97-SER132 | |
| B | VAL97-SER132 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 18 |
| Details | INTRAMEM: Helical; Name=Helix 2A => ECO:0000305 |
| Chain | Residue | Details |
| Y | VAL97-LYS106 | |
| B | VAL97-LYS106 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 10 |
| Details | INTRAMEM: INTRAMEM => ECO:0000305 |
| Chain | Residue | Details |
| Y | LYS107-GLY112 | |
| B | LYS107-GLY112 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 38 |
| Details | INTRAMEM: Helical; Name=Helix 2B => ECO:0000305 |
| Chain | Residue | Details |
| Y | ILE77-THR96 | |
| B | ILE77-THR96 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 30 |
| Details | TRANSMEM: Helical; Name=3 => ECO:0000305 |
| Chain | Residue | Details |
| Y | ILE116-GLN131 | |
| B | ILE116-GLN131 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 160 |
| Details | TOPO_DOM: Periplasmic => ECO:0000305 |
| Chain | Residue | Details |
| Y | ALA288-GLY313 | |
| Y | LYS396 | |
| B | SER132-LEU164 | |
| B | PRO201-VAL223 | |
| B | ALA288-GLY313 | |
| B | LYS396 | |
| Y | SER132-LEU164 | |
| Y | PRO201-VAL223 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 26 |
| Details | TRANSMEM: Helical; Name=4 => ECO:0000305 |
| Chain | Residue | Details |
| Y | VAL165-ILE178 | |
| B | VAL165-ILE178 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 32 |
| Details | TRANSMEM: Helical; Name=5 => ECO:0000305 |
| Chain | Residue | Details |
| Y | GLY184-PRO200 | |
| B | GLY184-PRO200 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 26 |
| Details | TRANSMEM: Helical; Name=6 => ECO:0000305 |
| Chain | Residue | Details |
| Y | ALA224-VAL237 | |
| B | ALA224-VAL237 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 26 |
| Details | TRANSMEM: Helical; Name=7 => ECO:0000305 |
| Chain | Residue | Details |
| Y | VAL274-PRO287 | |
| B | VAL274-PRO287 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 30 |
| Details | TRANSMEM: Helical; Name=8 => ECO:0000305 |
| Chain | Residue | Details |
| Y | GLN314-CYS329 | |
| B | GLN314-CYS329 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 28 |
| Details | TRANSMEM: Helical; Name=9 => ECO:0000305 |
| Chain | Residue | Details |
| Y | ILE381-MET395 | |
| B | ILE381-MET395 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 32 |
| Details | TRANSMEM: Helical; Name=10 => ECO:0000305 |
| Chain | Residue | Details |
| Y | VAL397-ILE413 | |
| B | VAL397-ILE413 |
