2AE3
Glutaryl 7-Aminocephalosporanic Acid Acylase: mutational study of activation mechanism
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016811 | molecular_function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides |
| A | 0017000 | biological_process | antibiotic biosynthetic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0017000 | biological_process | antibiotic biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 301 |
| Chain | Residue |
| A | LEU148 |
| A | SER152 |
| A | PRO162 |
| A | HOH324 |
| B | PHE31 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Nucleophile |
| Chain | Residue | Details |
| B | SER1 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: ACT_SITE => ECO:0000255 |
| Chain | Residue | Details |
| B | HIS23 | |
| B | GLU455 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 3s8r |
| Chain | Residue | Details |
| B | SER1 | |
| B | ASN244 | |
| B | VAL70 |
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 288 |
| Chain | Residue | Details |
| B | SER1 | activator, covalently attached, hydrogen bond acceptor, hydrogen bond donor, increase electrophilicity, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor |
| B | HIS23 | activator, electrostatic stabiliser, increase acidity, steric role |
| B | VAL70 | electrostatic stabiliser |
| B | ASN244 | electrostatic stabiliser |
| B | GLU455 | activator, electrostatic stabiliser, increase acidity, steric role |
