2A89
Monomeric Sarcosine Oxidase: Structure of a covalently flavinylated amine oxidizing enzyme
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0008115 | molecular_function | sarcosine oxidase activity |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0008115 | molecular_function | sarcosine oxidase activity |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PO4 B 1401 |
| Chain | Residue |
| B | HIS53 |
| B | LEU113 |
| B | THR114 |
| B | GLU141 |
| B | PRO142 |
| B | ASN143 |
| B | SER144 |
| B | HOH2477 |
| B | HOH2782 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CL A 1402 |
| Chain | Residue |
| A | TYR317 |
| A | THR318 |
| A | GLY344 |
| A | FCG1400 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE CL A 1403 |
| Chain | Residue |
| A | ASN293 |
| A | HOH1420 |
| A | HOH1481 |
| B | ASN293 |
| B | HOH2425 |
| B | HOH2433 |
| B | HOH2729 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CL B 2402 |
| Chain | Residue |
| B | TYR317 |
| B | THR318 |
| B | SER343 |
| B | GLY344 |
| B | FCG2400 |
| B | HOH2413 |
| site_id | AC5 |
| Number of Residues | 40 |
| Details | BINDING SITE FOR RESIDUE FCG A 1400 |
| Chain | Residue |
| A | GLY10 |
| A | GLY12 |
| A | SER13 |
| A | MET14 |
| A | VAL32 |
| A | ASP33 |
| A | ALA34 |
| A | PHE35 |
| A | HIS39 |
| A | GLY42 |
| A | SER43 |
| A | HIS44 |
| A | ARG49 |
| A | ILE50 |
| A | ARG172 |
| A | VAL173 |
| A | SER200 |
| A | MET201 |
| A | GLY202 |
| A | TRP204 |
| A | LEU208 |
| A | VAL225 |
| A | TYR254 |
| A | PHE256 |
| A | CYS315 |
| A | MET316 |
| A | TYR317 |
| A | PHE342 |
| A | GLY344 |
| A | HIS345 |
| A | GLY346 |
| A | PHE347 |
| A | LYS348 |
| A | CL1402 |
| A | HOH1422 |
| A | HOH1431 |
| A | HOH1440 |
| A | HOH1458 |
| A | HOH1542 |
| A | HOH1636 |
| site_id | AC6 |
| Number of Residues | 40 |
| Details | BINDING SITE FOR RESIDUE FCG B 2400 |
| Chain | Residue |
| B | LYS348 |
| B | CL2402 |
| B | HOH2403 |
| B | HOH2409 |
| B | HOH2411 |
| B | HOH2426 |
| B | HOH2466 |
| B | GLY10 |
| B | GLY12 |
| B | SER13 |
| B | MET14 |
| B | VAL32 |
| B | ASP33 |
| B | ALA34 |
| B | PHE35 |
| B | HIS39 |
| B | GLY42 |
| B | SER43 |
| B | HIS44 |
| B | ARG49 |
| B | ILE50 |
| B | THR171 |
| B | ARG172 |
| B | VAL173 |
| B | SER200 |
| B | MET201 |
| B | GLY202 |
| B | TRP204 |
| B | LEU208 |
| B | VAL225 |
| B | TYR254 |
| B | PHE256 |
| B | CYS315 |
| B | MET316 |
| B | TYR317 |
| B | PHE342 |
| B | GLY344 |
| B | HIS345 |
| B | GLY346 |
| B | PHE347 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 60 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"S-8alpha-FAD cysteine"} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 2gb0 |
| Chain | Residue | Details |
| A | HIS269 | |
| A | CYS315 | |
| A | ARG49 | |
| A | HIS45 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 2gb0 |
| Chain | Residue | Details |
| B | HIS269 | |
| B | CYS315 | |
| B | ARG49 | |
| B | HIS45 |
| site_id | CSA3 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 2gb0 |
| Chain | Residue | Details |
| A | GLY344 |
| site_id | CSA4 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 2gb0 |
| Chain | Residue | Details |
| B | GLY344 |
| site_id | MCSA1 |
| Number of Residues | 7 |
| Details | M-CSA 113 |
| Chain | Residue | Details |
| A | HIS45 | electrostatic stabiliser |
| A | THR48 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| A | ARG49 | electrostatic stabiliser, modifies pKa |
| A | LYS265 | hydrogen bond donor, proton acceptor, proton donor, proton relay |
| A | HIS269 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | CYS315 | activator, alter redox potential, covalently attached |
| A | LYS348 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA2 |
| Number of Residues | 7 |
| Details | M-CSA 113 |
| Chain | Residue | Details |
| B | HIS45 | electrostatic stabiliser |
| B | THR48 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| B | ARG49 | electrostatic stabiliser, modifies pKa |
| B | LYS265 | hydrogen bond donor, proton acceptor, proton donor, proton relay |
| B | HIS269 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| B | CYS315 | activator, alter redox potential, covalently attached |
| B | LYS348 | electrostatic stabiliser, hydrogen bond donor |
