2A3Z
Ternary complex of the WH2 domain of WASP with Actin-DNAse I
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0001725 | cellular_component | stress fiber |
| A | 0003785 | molecular_function | actin monomer binding |
| A | 0005509 | molecular_function | calcium ion binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005523 | molecular_function | tropomyosin binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005856 | cellular_component | cytoskeleton |
| A | 0005865 | cellular_component | striated muscle thin filament |
| A | 0005884 | cellular_component | actin filament |
| A | 0010628 | biological_process | positive regulation of gene expression |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0019904 | molecular_function | protein domain specific binding |
| A | 0030027 | cellular_component | lamellipodium |
| A | 0030041 | biological_process | actin filament polymerization |
| A | 0030175 | cellular_component | filopodium |
| A | 0030240 | biological_process | skeletal muscle thin filament assembly |
| A | 0031013 | molecular_function | troponin I binding |
| A | 0031432 | molecular_function | titin binding |
| A | 0031941 | cellular_component | filamentous actin |
| A | 0032036 | molecular_function | myosin heavy chain binding |
| A | 0032432 | cellular_component | actin filament bundle |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0044297 | cellular_component | cell body |
| A | 0048306 | molecular_function | calcium-dependent protein binding |
| A | 0048741 | biological_process | skeletal muscle fiber development |
| A | 0051017 | biological_process | actin filament bundle assembly |
| A | 0090131 | biological_process | mesenchyme migration |
| A | 0098723 | cellular_component | skeletal muscle myofibril |
| A | 0140660 | molecular_function | cytoskeletal motor activator activity |
| B | 0002283 | biological_process | neutrophil activation involved in immune response |
| B | 0002673 | biological_process | regulation of acute inflammatory response |
| B | 0003677 | molecular_function | DNA binding |
| B | 0003779 | molecular_function | actin binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004519 | molecular_function | endonuclease activity |
| B | 0004530 | molecular_function | deoxyribonuclease I activity |
| B | 0004536 | molecular_function | DNA nuclease activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005576 | cellular_component | extracellular region |
| B | 0005634 | cellular_component | nucleus |
| B | 0005635 | cellular_component | nuclear envelope |
| B | 0006308 | biological_process | DNA catabolic process |
| B | 0006915 | biological_process | apoptotic process |
| B | 0031410 | cellular_component | cytoplasmic vesicle |
| B | 0042588 | cellular_component | zymogen granule |
| B | 0070948 | biological_process | regulation of neutrophil mediated cytotoxicity |
| C | 0003779 | molecular_function | actin binding |
Functional Information from PROSITE/UniProt
| site_id | PS00406 |
| Number of Residues | 11 |
| Details | ACTINS_1 Actins signature 1. YVGDEAQs.KRG |
| Chain | Residue | Details |
| A | TYR53-GLY63 |
| site_id | PS00432 |
| Number of Residues | 9 |
| Details | ACTINS_2 Actins signature 2. WITKqEYDE |
| Chain | Residue | Details |
| A | TRP356-GLU364 |
| site_id | PS00918 |
| Number of Residues | 8 |
| Details | DNASE_I_2 Deoxyribonuclease I signature 2. GDFNAdCS |
| Chain | Residue | Details |
| B | GLY167-SER174 |
| site_id | PS00919 |
| Number of Residues | 21 |
| Details | DNASE_I_1 Deoxyribonuclease I signature 1. IVALHSAPsdavaEINsLyDV |
| Chain | Residue | Details |
| B | ILE130-VAL150 |
| site_id | PS01132 |
| Number of Residues | 13 |
| Details | ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR |
| Chain | Residue | Details |
| A | LEU104-ARG116 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: ACT_SITE => ECO:0000269|PubMed:2395459 |
| Chain | Residue | Details |
| B | GLU78 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: ACT_SITE => ECO:0000269|PubMed:2395459, ECO:0000269|PubMed:4976790 |
| Chain | Residue | Details |
| B | HIS134 | |
| A | MET47 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | SITE: Involved in actin-binding => ECO:0000269|PubMed:2395459 |
| Chain | Residue | Details |
| B | GLU13 | |
| B | VAL67 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | SITE: Nitration by tetranitromethane destroys a Ca(2+) binding site and inactivates enzyme |
| Chain | Residue | Details |
| B | TYR65 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:1748997, ECO:0000269|PubMed:3560229 |
| Chain | Residue | Details |
| B | ASN18 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: ADP-ribosylarginine; by SpvB => ECO:0000305|PubMed:16905096 |
| Chain | Residue | Details |
| A | ARG177 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1dnk |
| Chain | Residue | Details |
| B | ASP212 | |
| B | HIS134 | |
| B | GLU78 | |
| B | HIS252 |
| site_id | MCSA1 |
| Number of Residues | 7 |
| Details | M-CSA 41 |
| Chain | Residue | Details |
| B | GLU39 | metal ligand |
| B | TYR76 | electrostatic stabiliser |
| B | GLU78 | electrostatic stabiliser, increase acidity, increase basicity |
| B | HIS134 | proton acceptor, proton donor |
| B | ASP168 | metal ligand |
| B | ASP212 | electrostatic stabiliser, increase acidity, increase basicity |
| B | HIS252 | proton acceptor, proton donor |
