1ZLQ
Crystallographic and spectroscopic evidence for high affinity binding of Fe EDTA (H2O)- to the periplasmic nickel transporter NikA
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0015675 | biological_process | nickel cation transport |
| A | 0015833 | biological_process | peptide transport |
| A | 0016020 | cellular_component | membrane |
| A | 0016151 | molecular_function | nickel cation binding |
| A | 0020037 | molecular_function | heme binding |
| A | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| A | 0042597 | cellular_component | periplasmic space |
| A | 0043190 | cellular_component | ATP-binding cassette (ABC) transporter complex |
| A | 0046914 | molecular_function | transition metal ion binding |
| A | 0050919 | biological_process | negative chemotaxis |
| A | 0051540 | molecular_function | metal cluster binding |
| A | 0055085 | biological_process | transmembrane transport |
| A | 0098716 | biological_process | nickel cation import across plasma membrane |
| A | 1904680 | molecular_function | peptide transmembrane transporter activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0015675 | biological_process | nickel cation transport |
| B | 0015833 | biological_process | peptide transport |
| B | 0016020 | cellular_component | membrane |
| B | 0016151 | molecular_function | nickel cation binding |
| B | 0020037 | molecular_function | heme binding |
| B | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| B | 0042597 | cellular_component | periplasmic space |
| B | 0043190 | cellular_component | ATP-binding cassette (ABC) transporter complex |
| B | 0046914 | molecular_function | transition metal ion binding |
| B | 0050919 | biological_process | negative chemotaxis |
| B | 0051540 | molecular_function | metal cluster binding |
| B | 0055085 | biological_process | transmembrane transport |
| B | 0098716 | biological_process | nickel cation import across plasma membrane |
| B | 1904680 | molecular_function | peptide transmembrane transporter activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE FE A 1501 |
| Chain | Residue |
| A | EDT1513 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE FE B 1502 |
| Chain | Residue |
| B | EDT1511 |
| B | HOH1514 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT A 1502 |
| Chain | Residue |
| A | PRO11 |
| A | GLY219 |
| A | ASN220 |
| A | GLY222 |
| A | LEU223 |
| A | HOH1927 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT B 1503 |
| Chain | Residue |
| B | TYR382 |
| B | GLN385 |
| B | PHE394 |
| B | HIS395 |
| B | ARG396 |
| B | HOH1609 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ACT A 1503 |
| Chain | Residue |
| A | LYS52 |
| A | ARG68 |
| A | ASP69 |
| A | ASP70 |
| A | HOH1883 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE ACT B 1504 |
| Chain | Residue |
| B | THR23 |
| B | GLN26 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT A 1504 |
| Chain | Residue |
| A | ASN261 |
| A | GLU262 |
| A | LEU263 |
| A | HOH1576 |
| A | HOH1589 |
| A | HOH1877 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE ACT A 1505 |
| Chain | Residue |
| A | ASN235 |
| A | ALA237 |
| A | PHE419 |
| A | GLN423 |
| A | HOH1600 |
| A | HOH1709 |
| site_id | AC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACT A 1506 |
| Chain | Residue |
| A | ASN482 |
| A | GLN496 |
| A | HOH1801 |
| B | ASP43 |
| site_id | BC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ACT A 1507 |
| Chain | Residue |
| A | GLN385 |
| A | PHE394 |
| A | HIS395 |
| A | ARG396 |
| A | HOH1648 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACT B 1505 |
| Chain | Residue |
| B | VAL273 |
| B | LYS275 |
| B | LYS276 |
| B | TYR310 |
| site_id | BC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1508 |
| Chain | Residue |
| A | ARG384 |
| A | HOH1805 |
| A | HOH1897 |
| A | HOH1908 |
| B | LEU430 |
| B | ARG457 |
| site_id | BC4 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL A 1509 |
| Chain | Residue |
| A | GLN26 |
| site_id | BC5 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL A 1510 |
| Chain | Residue |
| A | ASP331 |
| A | ARG365 |
| site_id | BC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CL A 1511 |
| Chain | Residue |
| A | ARG97 |
| A | HOH1642 |
| B | LYS306 |
| B | LYS314 |
| site_id | BC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CL B 1506 |
| Chain | Residue |
| B | MET27 |
| B | PHE28 |
| B | THR490 |
| site_id | BC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CL A 1512 |
| Chain | Residue |
| A | PHE229 |
| A | SER233 |
| B | ASN302 |
| site_id | BC9 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE EDT A 1513 |
| Chain | Residue |
| A | TYR22 |
| A | MET27 |
| A | ARG97 |
| A | TRP100 |
| A | ARG137 |
| A | TRP398 |
| A | HIS416 |
| A | THR490 |
| A | FE1501 |
| A | HOH1647 |
| A | HOH1747 |
| A | HOH1903 |
| A | HOH1937 |
| site_id | CC1 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE EDT B 1511 |
| Chain | Residue |
| B | TYR22 |
| B | ARG97 |
| B | TRP100 |
| B | ARG137 |
| B | TRP398 |
| B | THR490 |
| B | FE1502 |
| B | HOH1514 |
| B | HOH1520 |
| B | HOH1603 |
| B | HOH1613 |
| B | HOH1868 |
| site_id | CC2 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE DTT A 1514 |
| Chain | Residue |
| A | ILE359 |
| A | ALA362 |
| A | HOH1972 |
| A | HOH1976 |
| A | HOH1987 |
| A | ARG89 |
| A | LEU92 |
| A | ARG95 |
| A | VAL108 |
| A | ASP109 |
| A | VAL110 |
| A | ASN281 |
| A | GLU358 |
| site_id | CC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 1515 |
| Chain | Residue |
| A | LEU167 |
| A | GLN168 |
| A | HOH1683 |
| B | THR211 |
| B | ASP213 |
| site_id | CC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL B 1512 |
| Chain | Residue |
| B | TRP10 |
| B | PRO11 |
| B | GLY219 |
| B | ASN220 |
| B | GLY222 |
| B | LEU223 |
| B | HOH1847 |
| B | HOH1862 |
| site_id | CC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 1516 |
| Chain | Residue |
| A | LYS148 |
| A | ASN149 |
| A | LYS157 |
| A | HOH1887 |
| A | HOH1931 |
| site_id | CC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 1517 |
| Chain | Residue |
| A | LYS111 |
| A | GLU358 |
| A | GLN361 |
| A | SER372 |
| A | LEU373 |
| site_id | CC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL B 1513 |
| Chain | Residue |
| B | SER57 |
| B | ASP59 |
| B | LYS61 |
| B | HOH1884 |
Functional Information from PROSITE/UniProt
| site_id | PS01040 |
| Number of Residues | 23 |
| Details | SBP_BACTERIAL_5 Bacterial extracellular solute-binding proteins, family 5 signature. AkswthseDgkTWtFtLRDDVKF |
| Chain | Residue | Details |
| A | ALA51-PHE73 |
