1ZFN

Structural Analysis of Escherichia coli ThiF

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0004792	molecular_function	thiosulfate-cyanide sulfurtransferase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0008146	molecular_function	sulfotransferase activity
A	0008270	molecular_function	zinc ion binding
A	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
A	0009228	biological_process	thiamine biosynthetic process
A	0009229	biological_process	thiamine diphosphate biosynthetic process
A	0016740	molecular_function	transferase activity
A	0016779	molecular_function	nucleotidyltransferase activity
A	0042803	molecular_function	protein homodimerization activity
A	0046872	molecular_function	metal ion binding
A	0052837	biological_process	thiazole biosynthetic process
A	0070733	molecular_function	AMPylase activity
B	0000166	molecular_function	nucleotide binding
B	0004792	molecular_function	thiosulfate-cyanide sulfurtransferase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0008146	molecular_function	sulfotransferase activity
B	0008270	molecular_function	zinc ion binding
B	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
B	0009228	biological_process	thiamine biosynthetic process
B	0009229	biological_process	thiamine diphosphate biosynthetic process
B	0016740	molecular_function	transferase activity
B	0016779	molecular_function	nucleotidyltransferase activity
B	0042803	molecular_function	protein homodimerization activity
B	0046872	molecular_function	metal ion binding
B	0052837	biological_process	thiazole biosynthetic process
B	0070733	molecular_function	AMPylase activity
C	0000166	molecular_function	nucleotide binding
C	0004792	molecular_function	thiosulfate-cyanide sulfurtransferase activity
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0008146	molecular_function	sulfotransferase activity
C	0008270	molecular_function	zinc ion binding
C	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
C	0009228	biological_process	thiamine biosynthetic process
C	0009229	biological_process	thiamine diphosphate biosynthetic process
C	0016740	molecular_function	transferase activity
C	0016779	molecular_function	nucleotidyltransferase activity
C	0042803	molecular_function	protein homodimerization activity
C	0046872	molecular_function	metal ion binding
C	0052837	biological_process	thiazole biosynthetic process
C	0070733	molecular_function	AMPylase activity
D	0000166	molecular_function	nucleotide binding
D	0004792	molecular_function	thiosulfate-cyanide sulfurtransferase activity
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0008146	molecular_function	sulfotransferase activity
D	0008270	molecular_function	zinc ion binding
D	0008641	molecular_function	ubiquitin-like modifier activating enzyme activity
D	0009228	biological_process	thiamine biosynthetic process
D	0009229	biological_process	thiamine diphosphate biosynthetic process
D	0016740	molecular_function	transferase activity
D	0016779	molecular_function	nucleotidyltransferase activity
D	0042803	molecular_function	protein homodimerization activity
D	0046872	molecular_function	metal ion binding
D	0052837	biological_process	thiazole biosynthetic process
D	0070733	molecular_function	AMPylase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 252

Chain	Residue
A	CYS169
A	CYS172
A	CYS240
A	CYS243

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN B 252

Chain	Residue
B	CYS169
B	CYS172
B	CYS240
B	VAL242
B	CYS243

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 345

Chain	Residue
C	CYS169
C	CYS172
C	CYS240
C	CYS243

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 445

Chain	Residue
D	CYS169
D	CYS172
D	CYS240
D	CYS243

---

site_id	AC5
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ATP C 346

Chain	Residue
C	ILE34
C	GLY35
C	GLY37
C	GLY38
C	ASP59
C	ASP61
C	SER66
C	ASN67
C	ARG70
C	GLN71
C	LYS83
C	ARG106
C	LEU107
C	CYS125
C	THR126
C	ASP127
C	THR131
D	ARG11

---

site_id	AC6
Number of Residues	19
Details	BINDING SITE FOR RESIDUE ATP D 446

Chain	Residue
C	ARG11
D	ILE34
D	GLY35
D	GLY37
D	GLY38
D	ALA58
D	ASP59
D	ASP61
D	SER66
D	ASN67
D	ARG70
D	GLN71
D	LYS83
D	ARG106
D	LEU107
D	CYS125
D	THR126
D	ASP127
D	THR131

---

site_id	AC7
Number of Residues	17
Details	BINDING SITE FOR RESIDUE ATP A 253

Chain	Residue
A	ILE34
A	GLY35
A	GLY37
A	GLY38
A	ASP59
A	ASP61
A	ARG70
A	GLN71
A	LYS83
A	GLN105
A	ARG106
A	LEU107
A	CYS125
A	THR126
A	ASP127
A	THR131
B	ARG11

---

site_id	AC8
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ATP B 253

Chain	Residue
A	ARG11
B	GLY35
B	GLY37
B	GLY38
B	ASP59
B	ASP61
B	SER66
B	ASN67
B	ARG70
B	GLN71
B	LYS83
B	GLN105
B	ARG106
B	LEU107
B	CYS125
B	THR126
B	ASP127
B	THR131

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Glycyl persulfide ester intermediate"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	40
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"16388576","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Cross-link: {"description":"Glycyl cysteine dithioester (Cys-Gly) (interchain with G-Cter in ThiS)"}

Chain

Residue

Details

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