1Z32

Structure-function relationships in human salivary alpha-amylase: Role of aromatic residues

Functional Information from GO Data

Chain	GOid	namespace	contents
X	0003824	molecular_function	catalytic activity
X	0004556	molecular_function	alpha-amylase activity
X	0005509	molecular_function	calcium ion binding
X	0005576	cellular_component	extracellular region
X	0005615	cellular_component	extracellular space
X	0005975	biological_process	carbohydrate metabolic process
X	0009311	biological_process	oligosaccharide metabolic process
X	0016160	molecular_function	amylase activity
X	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
X	0031404	molecular_function	chloride ion binding
X	0043169	molecular_function	cation binding
X	0046872	molecular_function	metal ion binding
X	0070062	cellular_component	extracellular exosome

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
X	ASP197

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor

Chain	Residue	Details
X	GLU233

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site_id	SWS_FT_FI3
Number of Residues	7
Details	BINDING: BINDING => ECO:0000269|PubMed:12527308, ECO:0000269|PubMed:15299664, ECO:0007744|PDB:1SMD

Chain	Residue	Details
X	ASN100
X	ARG158
X	ASP167
X	ARG195
X	HIS201
X	ASN298
X	ARG337

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site_id	SWS_FT_FI4
Number of Residues	1
Details	SITE: Transition state stabilizer => ECO:0000250|UniProtKB:P04746

Chain	Residue	Details
X	ASP300

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site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Pyrrolidone carboxylic acid => ECO:0000250|UniProtKB:P00687

Chain	Residue	Details
X	PCA1

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site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Deamidated asparagine; partial => ECO:0000269|PubMed:1710976

Chain	Residue	Details
X	ASN350
X	ASN459

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site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Deamidated asparagine; partial; alternate => ECO:0000269|PubMed:1710976

Chain	Residue	Details
X	ASN412

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site_id	SWS_FT_FI8
Number of Residues	1
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16740002, ECO:0000269|PubMed:1710976

Chain	Residue	Details
X	ASN412

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site_id	SWS_FT_FI9
Number of Residues	1
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
X	ASN461

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
X	ASP300
X	ASP197
X	GLU233

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site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
X	ASP197
X	GLU233

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site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1amy

Chain	Residue	Details
X	ASP236
X	ASP197

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