Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1YKK

Protocatechuate 3,4-Dioxygenase Y408C Mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0005506	molecular_function	iron ion binding
A	0006725	biological_process	cellular aromatic compound metabolic process
A	0008199	molecular_function	ferric iron binding
A	0016491	molecular_function	oxidoreductase activity
A	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
A	0018578	molecular_function	protocatechuate 3,4-dioxygenase activity
A	0019439	biological_process	aromatic compound catabolic process
A	0042952	biological_process	beta-ketoadipate pathway
A	0051213	molecular_function	dioxygenase activity
B	0003824	molecular_function	catalytic activity
B	0005506	molecular_function	iron ion binding
B	0006725	biological_process	cellular aromatic compound metabolic process
B	0008199	molecular_function	ferric iron binding
B	0016491	molecular_function	oxidoreductase activity
B	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
B	0018578	molecular_function	protocatechuate 3,4-dioxygenase activity
B	0019439	biological_process	aromatic compound catabolic process
B	0019619	biological_process	3,4-dihydroxybenzoate catabolic process
B	0042952	biological_process	beta-ketoadipate pathway
B	0046872	molecular_function	metal ion binding
B	0051213	molecular_function	dioxygenase activity
C	0003824	molecular_function	catalytic activity
C	0005506	molecular_function	iron ion binding
C	0006725	biological_process	cellular aromatic compound metabolic process
C	0008199	molecular_function	ferric iron binding
C	0016491	molecular_function	oxidoreductase activity
C	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
C	0018578	molecular_function	protocatechuate 3,4-dioxygenase activity
C	0019439	biological_process	aromatic compound catabolic process
C	0042952	biological_process	beta-ketoadipate pathway
C	0051213	molecular_function	dioxygenase activity
D	0003824	molecular_function	catalytic activity
D	0005506	molecular_function	iron ion binding
D	0006725	biological_process	cellular aromatic compound metabolic process
D	0008199	molecular_function	ferric iron binding
D	0016491	molecular_function	oxidoreductase activity
D	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
D	0018578	molecular_function	protocatechuate 3,4-dioxygenase activity
D	0019439	biological_process	aromatic compound catabolic process
D	0019619	biological_process	3,4-dihydroxybenzoate catabolic process
D	0042952	biological_process	beta-ketoadipate pathway
D	0046872	molecular_function	metal ion binding
D	0051213	molecular_function	dioxygenase activity
E	0003824	molecular_function	catalytic activity
E	0005506	molecular_function	iron ion binding
E	0006725	biological_process	cellular aromatic compound metabolic process
E	0008199	molecular_function	ferric iron binding
E	0016491	molecular_function	oxidoreductase activity
E	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
E	0018578	molecular_function	protocatechuate 3,4-dioxygenase activity
E	0019439	biological_process	aromatic compound catabolic process
E	0042952	biological_process	beta-ketoadipate pathway
E	0051213	molecular_function	dioxygenase activity
F	0003824	molecular_function	catalytic activity
F	0005506	molecular_function	iron ion binding
F	0006725	biological_process	cellular aromatic compound metabolic process
F	0008199	molecular_function	ferric iron binding
F	0016491	molecular_function	oxidoreductase activity
F	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
F	0018578	molecular_function	protocatechuate 3,4-dioxygenase activity
F	0019439	biological_process	aromatic compound catabolic process
F	0019619	biological_process	3,4-dihydroxybenzoate catabolic process
F	0042952	biological_process	beta-ketoadipate pathway
F	0046872	molecular_function	metal ion binding
F	0051213	molecular_function	dioxygenase activity
G	0003824	molecular_function	catalytic activity
G	0005506	molecular_function	iron ion binding
G	0006725	biological_process	cellular aromatic compound metabolic process
G	0008199	molecular_function	ferric iron binding
G	0016491	molecular_function	oxidoreductase activity
G	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
G	0018578	molecular_function	protocatechuate 3,4-dioxygenase activity
G	0019439	biological_process	aromatic compound catabolic process
G	0042952	biological_process	beta-ketoadipate pathway
G	0051213	molecular_function	dioxygenase activity
H	0003824	molecular_function	catalytic activity
H	0005506	molecular_function	iron ion binding
H	0006725	biological_process	cellular aromatic compound metabolic process
H	0008199	molecular_function	ferric iron binding
H	0016491	molecular_function	oxidoreductase activity
H	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
H	0018578	molecular_function	protocatechuate 3,4-dioxygenase activity
H	0019439	biological_process	aromatic compound catabolic process
H	0019619	biological_process	3,4-dihydroxybenzoate catabolic process
H	0042952	biological_process	beta-ketoadipate pathway
H	0046872	molecular_function	metal ion binding
H	0051213	molecular_function	dioxygenase activity
I	0003824	molecular_function	catalytic activity
I	0005506	molecular_function	iron ion binding
I	0006725	biological_process	cellular aromatic compound metabolic process
I	0008199	molecular_function	ferric iron binding
I	0016491	molecular_function	oxidoreductase activity
I	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
I	0018578	molecular_function	protocatechuate 3,4-dioxygenase activity
I	0019439	biological_process	aromatic compound catabolic process
I	0042952	biological_process	beta-ketoadipate pathway
I	0051213	molecular_function	dioxygenase activity
J	0003824	molecular_function	catalytic activity
J	0005506	molecular_function	iron ion binding
J	0006725	biological_process	cellular aromatic compound metabolic process
J	0008199	molecular_function	ferric iron binding
J	0016491	molecular_function	oxidoreductase activity
J	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
J	0018578	molecular_function	protocatechuate 3,4-dioxygenase activity
J	0019439	biological_process	aromatic compound catabolic process
J	0019619	biological_process	3,4-dihydroxybenzoate catabolic process
J	0042952	biological_process	beta-ketoadipate pathway
J	0046872	molecular_function	metal ion binding
J	0051213	molecular_function	dioxygenase activity
K	0003824	molecular_function	catalytic activity
K	0005506	molecular_function	iron ion binding
K	0006725	biological_process	cellular aromatic compound metabolic process
K	0008199	molecular_function	ferric iron binding
K	0016491	molecular_function	oxidoreductase activity
K	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
K	0018578	molecular_function	protocatechuate 3,4-dioxygenase activity
K	0019439	biological_process	aromatic compound catabolic process
K	0042952	biological_process	beta-ketoadipate pathway
K	0051213	molecular_function	dioxygenase activity
L	0003824	molecular_function	catalytic activity
L	0005506	molecular_function	iron ion binding
L	0006725	biological_process	cellular aromatic compound metabolic process
L	0008199	molecular_function	ferric iron binding
L	0016491	molecular_function	oxidoreductase activity
L	0016702	molecular_function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
L	0018578	molecular_function	protocatechuate 3,4-dioxygenase activity
L	0019439	biological_process	aromatic compound catabolic process
L	0019619	biological_process	3,4-dihydroxybenzoate catabolic process
L	0042952	biological_process	beta-ketoadipate pathway
L	0046872	molecular_function	metal ion binding
L	0051213	molecular_function	dioxygenase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE B 600

Chain	Residue
B	TYR447
B	HIS460
B	HIS462
B	HOH680

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE D 600

Chain	Residue
D	TYR447
D	HIS460
D	HIS462
D	HOH682

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE F 600

Chain	Residue
F	HIS460
F	HIS462
F	HOH682
F	TYR447

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE H 600

Chain	Residue
H	TYR447
H	HIS460
H	HIS462
H	HOH680

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE J 600

Chain	Residue
J	TYR447
J	HIS460
J	HIS462
J	HOH680

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE L 600

Chain	Residue
L	TYR447
L	HIS460
L	HIS462
L	HOH784

Functional Information from PROSITE/UniProt

site_id	PS00083
Number of Residues	29
Details	INTRADIOL_DIOXYGENAS Intradiol ring-cleavage dioxygenases signature. LlGqVydgnGhlVrdsfLEVwqadanGeY

Chain	Residue	Details
A	LEU51-TYR79

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269\|PubMed:7990141

Chain	Residue	Details
B	ARG409
B	PRO448
B	ILE461
B	PHE463
D	ARG409
D	PRO448
D	ILE461
D	PHE463
F	ARG409
F	PRO448
F	ILE461
F	PHE463
H	ARG409
H	PRO448
H	ILE461
H	PHE463
J	ARG409
J	PRO448
J	ILE461
J	PHE463
L	ARG409
L	PRO448
L	ILE461
L	PHE463

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 936

Chain	Residue	Details
B	ARG409	metal ligand
B	PRO448	metal ligand, proton shuttle (general acid/base)
B	PRO458	electrostatic stabiliser
B	ILE461	metal ligand
B	PHE463	metal ligand

site_id	MCSA2
Number of Residues	5
Details	M-CSA 936

Chain	Residue	Details
D	ARG409	metal ligand
D	PRO448	metal ligand, proton shuttle (general acid/base)
D	PRO458	electrostatic stabiliser
D	ILE461	metal ligand
D	PHE463	metal ligand

site_id	MCSA3
Number of Residues	5
Details	M-CSA 936

Chain	Residue	Details
F	ARG409	metal ligand
F	PRO448	metal ligand, proton shuttle (general acid/base)
F	PRO458	electrostatic stabiliser
F	ILE461	metal ligand
F	PHE463	metal ligand

site_id	MCSA4
Number of Residues	5
Details	M-CSA 936

Chain	Residue	Details
H	ARG409	metal ligand
H	PRO448	metal ligand, proton shuttle (general acid/base)
H	PRO458	electrostatic stabiliser
H	ILE461	metal ligand
H	PHE463	metal ligand

site_id	MCSA5
Number of Residues	5
Details	M-CSA 936

Chain	Residue	Details
J	ARG409	metal ligand
J	PRO448	metal ligand, proton shuttle (general acid/base)
J	PRO458	electrostatic stabiliser
J	ILE461	metal ligand
J	PHE463	metal ligand

site_id	MCSA6
Number of Residues	5
Details	M-CSA 936

Chain	Residue	Details
L	ARG409	metal ligand
L	PRO448	metal ligand, proton shuttle (general acid/base)
L	PRO458	electrostatic stabiliser
L	ILE461	metal ligand
L	PHE463	metal ligand

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)