1Y9M
Crystal structure of exo-inulinase from Aspergillus awamori in spacegroup P212121
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000272 | biological_process | polysaccharide catabolic process |
| A | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| A | 0004575 | molecular_function | sucrose alpha-glucosidase activity |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0005987 | biological_process | sucrose catabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| A | 0051669 | molecular_function | fructan beta-fructosidase activity |
| A | 0051670 | molecular_function | inulinase activity |
Functional Information from PROSITE/UniProt
| site_id | PS00609 |
| Number of Residues | 14 |
| Details | GLYCOSYL_HYDROL_F32 Glycosyl hydrolases family 32 active site. HfsPqknwMNDPNG |
| Chain | Residue | Details |
| A | HIS31-GLY44 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"PROSITE-ProRule","id":"PRU10067","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU10067","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 9 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"15522299","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1Y9G","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"39357631","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"15522299","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39357631","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1Y4W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1Y9G","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"39357631","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"15522299","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1Y9M","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PubMed","id":"15522299","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"39357631","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1Y4W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1Y9G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1Y9M","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | a catalytic site defined by CSA, PubMed 15522299 |
| Chain | Residue | Details |
| A | GLU241 | |
| A | ASP41 |
| site_id | MCSA1 |
| Number of Residues | 2 |
| Details | M-CSA 827 |
| Chain | Residue | Details |
| A | ASP41 | covalently attached, nucleofuge, nucleophile |
| A | GLU241 | activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor |
