1Y7T
Crystal structure of NAD(H)-depenent malate dehydrogenase complexed with NADPH
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0006099 | biological_process | tricarboxylic acid cycle |
| A | 0006107 | biological_process | oxaloacetate metabolic process |
| A | 0006108 | biological_process | malate metabolic process |
| A | 0006734 | biological_process | NADH metabolic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016615 | molecular_function | malate dehydrogenase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0019752 | biological_process | carboxylic acid metabolic process |
| A | 0030060 | molecular_function | L-malate dehydrogenase activity |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0006099 | biological_process | tricarboxylic acid cycle |
| B | 0006107 | biological_process | oxaloacetate metabolic process |
| B | 0006108 | biological_process | malate metabolic process |
| B | 0006734 | biological_process | NADH metabolic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016615 | molecular_function | malate dehydrogenase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0019752 | biological_process | carboxylic acid metabolic process |
| B | 0030060 | molecular_function | L-malate dehydrogenase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 25 |
| Details | BINDING SITE FOR RESIDUE NDP A 1334 |
| Chain | Residue |
| A | THR9 |
| A | GLY87 |
| A | ALA88 |
| A | GLN111 |
| A | GLY129 |
| A | ASN130 |
| A | LEU157 |
| A | SER240 |
| A | SER241 |
| A | ALA245 |
| A | TRS1335 |
| A | GLY10 |
| A | TRS1336 |
| A | HOH1369 |
| A | HOH1441 |
| A | HOH1454 |
| A | HOH1470 |
| A | HOH1497 |
| A | GLY13 |
| A | GLN14 |
| A | ILE15 |
| A | LEU40 |
| A | GLU41 |
| A | ILE42 |
| A | VAL86 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE TRS A 1335 |
| Chain | Residue |
| A | ASN130 |
| A | GLY230 |
| A | SER240 |
| A | NDP1334 |
| A | HOH1353 |
| A | HOH1366 |
| B | LYS220 |
| site_id | AC3 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE TRS A 1336 |
| Chain | Residue |
| A | ASN130 |
| A | LEU157 |
| A | ASP158 |
| A | ARG161 |
| A | HIS186 |
| A | NDP1334 |
| A | HOH1366 |
| A | HOH1382 |
| A | HOH1386 |
| A | HOH1394 |
| site_id | AC4 |
| Number of Residues | 28 |
| Details | BINDING SITE FOR RESIDUE NDP B 2334 |
| Chain | Residue |
| A | LYS220 |
| B | THR9 |
| B | GLY10 |
| B | GLY13 |
| B | GLN14 |
| B | ILE15 |
| B | LEU40 |
| B | GLU41 |
| B | ILE42 |
| B | VAL86 |
| B | GLY87 |
| B | ALA88 |
| B | GLN111 |
| B | GLY129 |
| B | ASN130 |
| B | LEU157 |
| B | SER240 |
| B | SER241 |
| B | ALA245 |
| B | TRS2335 |
| B | TRS2336 |
| B | HOH2363 |
| B | HOH2455 |
| B | HOH2474 |
| B | HOH2496 |
| B | HOH2515 |
| B | HOH2532 |
| B | HOH2540 |
| site_id | AC5 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE TRS B 2335 |
| Chain | Residue |
| A | LYS220 |
| A | HOH1510 |
| B | ASN130 |
| B | GLY230 |
| B | SER240 |
| B | NDP2334 |
| B | HOH2362 |
| B | HOH2368 |
| B | HOH2504 |
| site_id | AC6 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE TRS B 2336 |
| Chain | Residue |
| B | ASN130 |
| B | LEU157 |
| B | ASP158 |
| B | ARG161 |
| B | HIS186 |
| B | NDP2334 |
| B | HOH2341 |
| B | HOH2362 |
| B | HOH2420 |
| B | HOH2422 |
| B | HOH2424 |
Functional Information from PROSITE/UniProt
| site_id | PS00068 |
| Number of Residues | 13 |
| Details | MDH Malate dehydrogenase active site signature. MTRLDhnRAkaqL |
| Chain | Residue | Details |
| A | MET154-LEU166 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_01517 |
| Chain | Residue | Details |
| A | HIS186 | |
| B | HIS186 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01517, ECO:0000269|PubMed:16009341, ECO:0000269|PubMed:8471603 |
| Chain | Residue | Details |
| B | GLY10 | |
| B | VAL128 | |
| A | GLY10 | |
| A | VAL128 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01517 |
| Chain | Residue | Details |
| A | ARG91 | |
| A | ARG97 | |
| B | ASN130 | |
| B | ARG161 | |
| A | ASN104 | |
| A | ASN130 | |
| A | ARG161 | |
| B | ARG91 | |
| B | ARG97 | |
| B | ASN104 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01517, ECO:0000269|PubMed:16009341 |
| Chain | Residue | Details |
| A | GLN111 | |
| B | GLN111 |
