1Y5X
tRNA-guanine Transglycosylase (TGT) in complex with 6-Amino-4-[2-(4-methoxyphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0006400 | biological_process | tRNA modification |
| A | 0008033 | biological_process | tRNA processing |
| A | 0008479 | molecular_function | tRNA-guanosine(34) queuine transglycosylase activity |
| A | 0008616 | biological_process | queuosine biosynthetic process |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0016763 | molecular_function | pentosyltransferase activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0101030 | biological_process | tRNA-guanine transglycosylation |
| D | 0006400 | biological_process | tRNA modification |
| D | 0008033 | biological_process | tRNA processing |
| D | 0008479 | molecular_function | tRNA-guanosine(34) queuine transglycosylase activity |
| D | 0008616 | biological_process | queuosine biosynthetic process |
| D | 0016757 | molecular_function | glycosyltransferase activity |
| D | 0016763 | molecular_function | pentosyltransferase activity |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0101030 | biological_process | tRNA-guanine transglycosylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 400 |
| Chain | Residue |
| A | CYS318 |
| A | CYS320 |
| A | CYS323 |
| A | HIS349 |
| site_id | AC2 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE E89 A 500 |
| Chain | Residue |
| A | TYR106 |
| A | GLN107 |
| A | ASP156 |
| A | CYS158 |
| A | GLN203 |
| A | GLY229 |
| A | GLY230 |
| A | LEU231 |
| A | ALA232 |
| A | MET260 |
| A | GLY261 |
| A | HOH2218 |
| A | VAL45 |
| A | LEU68 |
| A | ASN70 |
| A | ASP102 |
| A | SER103 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN D 1400 |
| Chain | Residue |
| D | CYS1318 |
| D | CYS1320 |
| D | CYS1323 |
| D | HIS1349 |
| site_id | AC4 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE E89 D 1500 |
| Chain | Residue |
| D | LEU1068 |
| D | GLY1069 |
| D | ASP1102 |
| D | SER1103 |
| D | TYR1106 |
| D | ASP1156 |
| D | CYS1158 |
| D | ILE1201 |
| D | GLN1203 |
| D | GLY1229 |
| D | GLY1230 |
| D | LEU1231 |
| D | ALA1232 |
| D | TYR1258 |
| D | MET1260 |
| D | GLY1261 |
| D | ASP1280 |
| D | HOH2123 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000305|PubMed:12949492 |
| Chain | Residue | Details |
| A | SER103 | |
| D | SER1103 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: Nucleophile => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000305|PubMed:12949492 |
| Chain | Residue | Details |
| A | CYS281 | |
| D | CYS1281 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000269|PubMed:12949492 |
| Chain | Residue | Details |
| A | SER103 | |
| A | GLU157 | |
| A | GLY204 | |
| A | LEU231 | |
| D | SER1103 | |
| D | GLU1157 | |
| D | GLY1204 | |
| D | LEU1231 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000269|PubMed:10413112, ECO:0000269|PubMed:11178905, ECO:0000269|PubMed:11921407, ECO:0000269|PubMed:12646024, ECO:0000269|PubMed:12909636, ECO:0000269|PubMed:12949492, ECO:0000269|PubMed:14523925, ECO:0000269|PubMed:19627989, ECO:0000269|PubMed:8654383, ECO:0000269|PubMed:8961936 |
| Chain | Residue | Details |
| A | HIS319 | |
| A | ALA321 | |
| A | GLN324 | |
| A | ASN350 | |
| D | HIS1319 | |
| D | ALA1321 | |
| D | GLN1324 | |
| D | ASN1350 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1pud |
| Chain | Residue | Details |
| A | ASP102 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1pud |
| Chain | Residue | Details |
| D | ASP1102 |
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 881 |
| Chain | Residue | Details |
| A | SER103 | proton shuttle (general acid/base) |
| A | CYS281 | covalent catalysis |
| A | HIS319 | metal ligand |
| A | ALA321 | metal ligand |
| A | GLN324 | metal ligand |
| A | ASN350 | metal ligand |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 881 |
| Chain | Residue | Details |
| D | SER1103 | proton shuttle (general acid/base) |
| D | CYS1281 | covalent catalysis |
| D | HIS1319 | metal ligand |
| D | ALA1321 | metal ligand |
| D | GLN1324 | metal ligand |
| D | ASN1350 | metal ligand |
