1XEV

Crystal structure of human carbonic anhydrase II in a new crystal form

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002009	biological_process	morphogenesis of an epithelium
A	0004064	molecular_function	arylesterase activity
A	0004089	molecular_function	carbonate dehydratase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006730	biological_process	one-carbon metabolic process
A	0008270	molecular_function	zinc ion binding
A	0015670	biological_process	carbon dioxide transport
A	0016829	molecular_function	lyase activity
A	0018820	molecular_function	cyanamide hydratase activity
A	0032230	biological_process	positive regulation of synaptic transmission, GABAergic
A	0032849	biological_process	positive regulation of cellular pH reduction
A	0038166	biological_process	angiotensin-activated signaling pathway
A	0043209	cellular_component	myelin sheath
A	0044070	biological_process	regulation of monoatomic anion transport
A	0045177	cellular_component	apical part of cell
A	0046872	molecular_function	metal ion binding
A	0046903	biological_process	secretion
A	0051453	biological_process	regulation of intracellular pH
A	0070050	biological_process	neuron cellular homeostasis
A	0070062	cellular_component	extracellular exosome
A	2001150	biological_process	positive regulation of dipeptide transmembrane transport
A	2001225	biological_process	regulation of chloride transport
B	0002009	biological_process	morphogenesis of an epithelium
B	0004064	molecular_function	arylesterase activity
B	0004089	molecular_function	carbonate dehydratase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006730	biological_process	one-carbon metabolic process
B	0008270	molecular_function	zinc ion binding
B	0015670	biological_process	carbon dioxide transport
B	0016829	molecular_function	lyase activity
B	0018820	molecular_function	cyanamide hydratase activity
B	0032230	biological_process	positive regulation of synaptic transmission, GABAergic
B	0032849	biological_process	positive regulation of cellular pH reduction
B	0038166	biological_process	angiotensin-activated signaling pathway
B	0043209	cellular_component	myelin sheath
B	0044070	biological_process	regulation of monoatomic anion transport
B	0045177	cellular_component	apical part of cell
B	0046872	molecular_function	metal ion binding
B	0046903	biological_process	secretion
B	0051453	biological_process	regulation of intracellular pH
B	0070050	biological_process	neuron cellular homeostasis
B	0070062	cellular_component	extracellular exosome
B	2001150	biological_process	positive regulation of dipeptide transmembrane transport
B	2001225	biological_process	regulation of chloride transport
C	0002009	biological_process	morphogenesis of an epithelium
C	0004064	molecular_function	arylesterase activity
C	0004089	molecular_function	carbonate dehydratase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006730	biological_process	one-carbon metabolic process
C	0008270	molecular_function	zinc ion binding
C	0015670	biological_process	carbon dioxide transport
C	0016829	molecular_function	lyase activity
C	0018820	molecular_function	cyanamide hydratase activity
C	0032230	biological_process	positive regulation of synaptic transmission, GABAergic
C	0032849	biological_process	positive regulation of cellular pH reduction
C	0038166	biological_process	angiotensin-activated signaling pathway
C	0043209	cellular_component	myelin sheath
C	0044070	biological_process	regulation of monoatomic anion transport
C	0045177	cellular_component	apical part of cell
C	0046872	molecular_function	metal ion binding
C	0046903	biological_process	secretion
C	0051453	biological_process	regulation of intracellular pH
C	0070050	biological_process	neuron cellular homeostasis
C	0070062	cellular_component	extracellular exosome
C	2001150	biological_process	positive regulation of dipeptide transmembrane transport
C	2001225	biological_process	regulation of chloride transport
D	0002009	biological_process	morphogenesis of an epithelium
D	0004064	molecular_function	arylesterase activity
D	0004089	molecular_function	carbonate dehydratase activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006730	biological_process	one-carbon metabolic process
D	0008270	molecular_function	zinc ion binding
D	0015670	biological_process	carbon dioxide transport
D	0016829	molecular_function	lyase activity
D	0018820	molecular_function	cyanamide hydratase activity
D	0032230	biological_process	positive regulation of synaptic transmission, GABAergic
D	0032849	biological_process	positive regulation of cellular pH reduction
D	0038166	biological_process	angiotensin-activated signaling pathway
D	0043209	cellular_component	myelin sheath
D	0044070	biological_process	regulation of monoatomic anion transport
D	0045177	cellular_component	apical part of cell
D	0046872	molecular_function	metal ion binding
D	0046903	biological_process	secretion
D	0051453	biological_process	regulation of intracellular pH
D	0070050	biological_process	neuron cellular homeostasis
D	0070062	cellular_component	extracellular exosome
D	2001150	biological_process	positive regulation of dipeptide transmembrane transport
D	2001225	biological_process	regulation of chloride transport

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 262

Chain	Residue
A	HIS94
A	HIS96
A	HIS119
A	HOH395

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 562

Chain	Residue
B	HIS94
B	HIS96
B	HIS119
B	HOH713

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 862

Chain	Residue
C	HIS96
C	HIS119
C	HOH1031
C	HIS94

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 999

Chain	Residue
D	HIS94
D	HIS96
D	HIS119
D	HOH1140

---

Functional Information from PROSITE/UniProt

site_id	PS00162
Number of Residues	17
Details	ALPHA_CA_1 Alpha-carbonic anhydrases signature. SEHtVdkkkYaaELHLV

Chain	Residue	Details
A	SER105-VAL121

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton donor/acceptor => ECO:0000305|PubMed:15667203, ECO:0000305|PubMed:17330962

Chain	Residue	Details
A	ALA65
B	ALA65
C	ALA65
D	ALA65

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:11076507, ECO:0000269|PubMed:12499545, ECO:0000269|PubMed:1336460, ECO:0000269|PubMed:1433293, ECO:0000269|PubMed:1909891, ECO:0000269|PubMed:19583303, ECO:0000269|PubMed:3151019, ECO:0000269|PubMed:3151020, ECO:0000269|PubMed:4621826, ECO:0000269|PubMed:7761440, ECO:0000269|PubMed:7803386, ECO:0000269|PubMed:7901850, ECO:0000269|PubMed:8218160, ECO:0000269|PubMed:8262987, ECO:0000269|PubMed:8331673, ECO:0000269|PubMed:8399159, ECO:0000269|PubMed:8431430, ECO:0000269|PubMed:8451242, ECO:0000269|PubMed:8482389, ECO:0000269|PubMed:8639494, ECO:0000269|PubMed:8987974, ECO:0000269|PubMed:9398308, ECO:0000269|PubMed:9865942

Chain	Residue	Details
A	PHE95
B	PHE95
C	PHE95
D	PHE95

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269|PubMed:11076507, ECO:0000269|PubMed:12499545, ECO:0000269|PubMed:1336460, ECO:0000269|PubMed:1433293, ECO:0000269|PubMed:1909891, ECO:0000269|PubMed:19583303, ECO:0000269|PubMed:3151019, ECO:0000269|PubMed:3151020, ECO:0000269|PubMed:7761440, ECO:0000269|PubMed:7803386, ECO:0000269|PubMed:7901850, ECO:0000269|PubMed:8218160, ECO:0000269|PubMed:8262987, ECO:0000269|PubMed:8331673, ECO:0000269|PubMed:8399159, ECO:0000269|PubMed:8431430, ECO:0000269|PubMed:8451242, ECO:0000269|PubMed:8482389, ECO:0000269|PubMed:8639494, ECO:0000269|PubMed:8987974, ECO:0000269|PubMed:9398308, ECO:0000269|PubMed:9865942

Chain	Residue	Details
A	TRP97
A	LEU120
B	TRP97
B	LEU120
C	TRP97
C	LEU120
D	TRP97
D	LEU120

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:10550681, ECO:0000269|PubMed:19520834

Chain	Residue	Details
A	THR200
B	THR200
C	THR200
D	THR200

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	SITE: Fine-tunes the proton-transfer properties of H-64 => ECO:0000305|PubMed:17330962

Chain	Residue	Details
A	GLY8
B	GLY8
C	GLY8
D	GLY8

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	SITE: Fine-tunes the proton-transfer properties of H-64; involved in the binding of some activators, including histamine and L-histidine => ECO:0000269|PubMed:16214338, ECO:0000269|PubMed:9265618, ECO:0000305|PubMed:17330962

Chain	Residue	Details
A	GLY63
A	VAL68
B	GLY63
B	VAL68
C	GLY63
C	VAL68
D	GLY63
D	VAL68

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	SITE: Involved in the binding of some activators, including histamine and L-histidine => ECO:0000269|PubMed:16214338, ECO:0000269|PubMed:9265618, ECO:0000305|PubMed:17330962

Chain	Residue	Details
A	PHE93
B	PHE93
C	PHE93
D	PHE93

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P27139

Chain	Residue	Details
A	HIS3
B	HIS3
C	HIS3
D	HIS3

---

site_id	SWS_FT_FI9
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	ILE167
A	ALA174
B	ILE167
B	ALA174
C	ILE167
C	ALA174
D	ILE167
D	ALA174

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1ca2

Chain	Residue	Details
A	THR199
A	HIS64

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1ca2

Chain	Residue	Details
B	THR199
B	HIS64

---

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1ca2

Chain	Residue	Details
C	THR199
C	HIS64

---

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1ca2

Chain	Residue	Details
D	THR199
D	HIS64

---

site_id	MCSA1
Number of Residues	6
Details	M-CSA 216

Chain	Residue	Details
A	ALA65	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	PHE95	metal ligand
A	TRP97	metal ligand
A	HIS107	activator, electrostatic stabiliser, hydrogen bond acceptor
A	LEU120	metal ligand
A	THR200	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity

---

site_id	MCSA2
Number of Residues	6
Details	M-CSA 216

Chain	Residue	Details
B	ALA65	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	PHE95	metal ligand
B	TRP97	metal ligand
B	HIS107	activator, electrostatic stabiliser, hydrogen bond acceptor
B	LEU120	metal ligand
B	THR200	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity

---

site_id	MCSA3
Number of Residues	6
Details	M-CSA 216

Chain	Residue	Details
C	ALA65	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
C	PHE95	metal ligand
C	TRP97	metal ligand
C	HIS107	activator, electrostatic stabiliser, hydrogen bond acceptor
C	LEU120	metal ligand
C	THR200	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity

---

site_id	MCSA4
Number of Residues	6
Details	M-CSA 216

Chain	Residue	Details
D	ALA65	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
D	PHE95	metal ligand
D	TRP97	metal ligand
D	HIS107	activator, electrostatic stabiliser, hydrogen bond acceptor
D	LEU120	metal ligand
D	THR200	activator, electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, increase nucleophilicity

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