Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1XDM

Structure of human aldolase B associated with hereditary fructose intolerance (A149P), at 291K

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004332	molecular_function	fructose-bisphosphate aldolase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005815	cellular_component	microtubule organizing center
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0006000	biological_process	fructose metabolic process
A	0006001	biological_process	fructose catabolic process
A	0006094	biological_process	gluconeogenesis
A	0006096	biological_process	glycolytic process
A	0006116	biological_process	NADH oxidation
A	0008092	molecular_function	cytoskeletal protein binding
A	0016829	molecular_function	lyase activity
A	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
A	0032781	biological_process	positive regulation of ATP-dependent activity
A	0034451	cellular_component	centriolar satellite
A	0042802	molecular_function	identical protein binding
A	0051117	molecular_function	ATPase binding
A	0060090	molecular_function	molecular adaptor activity
A	0061609	molecular_function	fructose-1-phosphate aldolase activity
A	0061624	biological_process	fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate
A	0070061	molecular_function	fructose binding
A	0070062	cellular_component	extracellular exosome
A	0070072	biological_process	vacuolar proton-transporting V-type ATPase complex assembly
A	1905856	biological_process	negative regulation of pentose-phosphate shunt
B	0004332	molecular_function	fructose-bisphosphate aldolase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005815	cellular_component	microtubule organizing center
B	0005829	cellular_component	cytosol
B	0005856	cellular_component	cytoskeleton
B	0006000	biological_process	fructose metabolic process
B	0006001	biological_process	fructose catabolic process
B	0006094	biological_process	gluconeogenesis
B	0006096	biological_process	glycolytic process
B	0006116	biological_process	NADH oxidation
B	0008092	molecular_function	cytoskeletal protein binding
B	0016829	molecular_function	lyase activity
B	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
B	0032781	biological_process	positive regulation of ATP-dependent activity
B	0034451	cellular_component	centriolar satellite
B	0042802	molecular_function	identical protein binding
B	0051117	molecular_function	ATPase binding
B	0060090	molecular_function	molecular adaptor activity
B	0061609	molecular_function	fructose-1-phosphate aldolase activity
B	0061624	biological_process	fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate
B	0070061	molecular_function	fructose binding
B	0070062	cellular_component	extracellular exosome
B	0070072	biological_process	vacuolar proton-transporting V-type ATPase complex assembly
B	1905856	biological_process	negative regulation of pentose-phosphate shunt
C	0004332	molecular_function	fructose-bisphosphate aldolase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005815	cellular_component	microtubule organizing center
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0006000	biological_process	fructose metabolic process
C	0006001	biological_process	fructose catabolic process
C	0006094	biological_process	gluconeogenesis
C	0006096	biological_process	glycolytic process
C	0006116	biological_process	NADH oxidation
C	0008092	molecular_function	cytoskeletal protein binding
C	0016829	molecular_function	lyase activity
C	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
C	0032781	biological_process	positive regulation of ATP-dependent activity
C	0034451	cellular_component	centriolar satellite
C	0042802	molecular_function	identical protein binding
C	0051117	molecular_function	ATPase binding
C	0060090	molecular_function	molecular adaptor activity
C	0061609	molecular_function	fructose-1-phosphate aldolase activity
C	0061624	biological_process	fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate
C	0070061	molecular_function	fructose binding
C	0070062	cellular_component	extracellular exosome
C	0070072	biological_process	vacuolar proton-transporting V-type ATPase complex assembly
C	1905856	biological_process	negative regulation of pentose-phosphate shunt
D	0004332	molecular_function	fructose-bisphosphate aldolase activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005815	cellular_component	microtubule organizing center
D	0005829	cellular_component	cytosol
D	0005856	cellular_component	cytoskeleton
D	0006000	biological_process	fructose metabolic process
D	0006001	biological_process	fructose catabolic process
D	0006094	biological_process	gluconeogenesis
D	0006096	biological_process	glycolytic process
D	0006116	biological_process	NADH oxidation
D	0008092	molecular_function	cytoskeletal protein binding
D	0016829	molecular_function	lyase activity
D	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
D	0032781	biological_process	positive regulation of ATP-dependent activity
D	0034451	cellular_component	centriolar satellite
D	0042802	molecular_function	identical protein binding
D	0051117	molecular_function	ATPase binding
D	0060090	molecular_function	molecular adaptor activity
D	0061609	molecular_function	fructose-1-phosphate aldolase activity
D	0061624	biological_process	fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate
D	0070061	molecular_function	fructose binding
D	0070062	cellular_component	extracellular exosome
D	0070072	biological_process	vacuolar proton-transporting V-type ATPase complex assembly
D	1905856	biological_process	negative regulation of pentose-phosphate shunt
W	0004332	molecular_function	fructose-bisphosphate aldolase activity
W	0005515	molecular_function	protein binding
W	0005737	cellular_component	cytoplasm
W	0005815	cellular_component	microtubule organizing center
W	0005829	cellular_component	cytosol
W	0005856	cellular_component	cytoskeleton
W	0006000	biological_process	fructose metabolic process
W	0006001	biological_process	fructose catabolic process
W	0006094	biological_process	gluconeogenesis
W	0006096	biological_process	glycolytic process
W	0006116	biological_process	NADH oxidation
W	0008092	molecular_function	cytoskeletal protein binding
W	0016829	molecular_function	lyase activity
W	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
W	0032781	biological_process	positive regulation of ATP-dependent activity
W	0034451	cellular_component	centriolar satellite
W	0042802	molecular_function	identical protein binding
W	0051117	molecular_function	ATPase binding
W	0060090	molecular_function	molecular adaptor activity
W	0061609	molecular_function	fructose-1-phosphate aldolase activity
W	0061624	biological_process	fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate
W	0070061	molecular_function	fructose binding
W	0070062	cellular_component	extracellular exosome
W	0070072	biological_process	vacuolar proton-transporting V-type ATPase complex assembly
W	1905856	biological_process	negative regulation of pentose-phosphate shunt
X	0004332	molecular_function	fructose-bisphosphate aldolase activity
X	0005515	molecular_function	protein binding
X	0005737	cellular_component	cytoplasm
X	0005815	cellular_component	microtubule organizing center
X	0005829	cellular_component	cytosol
X	0005856	cellular_component	cytoskeleton
X	0006000	biological_process	fructose metabolic process
X	0006001	biological_process	fructose catabolic process
X	0006094	biological_process	gluconeogenesis
X	0006096	biological_process	glycolytic process
X	0006116	biological_process	NADH oxidation
X	0008092	molecular_function	cytoskeletal protein binding
X	0016829	molecular_function	lyase activity
X	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
X	0032781	biological_process	positive regulation of ATP-dependent activity
X	0034451	cellular_component	centriolar satellite
X	0042802	molecular_function	identical protein binding
X	0051117	molecular_function	ATPase binding
X	0060090	molecular_function	molecular adaptor activity
X	0061609	molecular_function	fructose-1-phosphate aldolase activity
X	0061624	biological_process	fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate
X	0070061	molecular_function	fructose binding
X	0070062	cellular_component	extracellular exosome
X	0070072	biological_process	vacuolar proton-transporting V-type ATPase complex assembly
X	1905856	biological_process	negative regulation of pentose-phosphate shunt
Y	0004332	molecular_function	fructose-bisphosphate aldolase activity
Y	0005515	molecular_function	protein binding
Y	0005737	cellular_component	cytoplasm
Y	0005815	cellular_component	microtubule organizing center
Y	0005829	cellular_component	cytosol
Y	0005856	cellular_component	cytoskeleton
Y	0006000	biological_process	fructose metabolic process
Y	0006001	biological_process	fructose catabolic process
Y	0006094	biological_process	gluconeogenesis
Y	0006096	biological_process	glycolytic process
Y	0006116	biological_process	NADH oxidation
Y	0008092	molecular_function	cytoskeletal protein binding
Y	0016829	molecular_function	lyase activity
Y	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
Y	0032781	biological_process	positive regulation of ATP-dependent activity
Y	0034451	cellular_component	centriolar satellite
Y	0042802	molecular_function	identical protein binding
Y	0051117	molecular_function	ATPase binding
Y	0060090	molecular_function	molecular adaptor activity
Y	0061609	molecular_function	fructose-1-phosphate aldolase activity
Y	0061624	biological_process	fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate
Y	0070061	molecular_function	fructose binding
Y	0070062	cellular_component	extracellular exosome
Y	0070072	biological_process	vacuolar proton-transporting V-type ATPase complex assembly
Y	1905856	biological_process	negative regulation of pentose-phosphate shunt
Z	0004332	molecular_function	fructose-bisphosphate aldolase activity
Z	0005515	molecular_function	protein binding
Z	0005737	cellular_component	cytoplasm
Z	0005815	cellular_component	microtubule organizing center
Z	0005829	cellular_component	cytosol
Z	0005856	cellular_component	cytoskeleton
Z	0006000	biological_process	fructose metabolic process
Z	0006001	biological_process	fructose catabolic process
Z	0006094	biological_process	gluconeogenesis
Z	0006096	biological_process	glycolytic process
Z	0006116	biological_process	NADH oxidation
Z	0008092	molecular_function	cytoskeletal protein binding
Z	0016829	molecular_function	lyase activity
Z	0030388	biological_process	fructose 1,6-bisphosphate metabolic process
Z	0032781	biological_process	positive regulation of ATP-dependent activity
Z	0034451	cellular_component	centriolar satellite
Z	0042802	molecular_function	identical protein binding
Z	0051117	molecular_function	ATPase binding
Z	0060090	molecular_function	molecular adaptor activity
Z	0061609	molecular_function	fructose-1-phosphate aldolase activity
Z	0061624	biological_process	fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate
Z	0070061	molecular_function	fructose binding
Z	0070062	cellular_component	extracellular exosome
Z	0070072	biological_process	vacuolar proton-transporting V-type ATPase complex assembly
Z	1905856	biological_process	negative regulation of pentose-phosphate shunt

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 W 364

Chain	Residue
W	GLN202
W	THR254
W	ARG258
X	GLN9
X	LYS12

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 364

Chain	Residue
A	LYS12
B	GLN202
B	THR254
B	ARG258

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 X 364

Chain	Residue
W	LYS12
X	GLN202
X	ARG258

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 Y 364

Chain	Residue
Y	GLN202
Y	ARG258
Z	LYS12

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 364

Chain	Residue
C	GLN202
C	ARG258
D	LYS12

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 364

Chain	Residue
A	GLN202
A	THR254
A	ARG258
A	HOH368
B	GLN9
B	LYS12

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 C 365

Chain	Residue
C	GLN9
C	LYS12
C	HOH368
D	GLN202
D	THR254
D	ARG258

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 Z 364

Chain	Residue
Y	GLN9
Y	LYS12
Z	GLN202
Z	ARG258
Z	HOH367

Functional Information from PROSITE/UniProt

site_id	PS00158
Number of Residues	11
Details	ALDOLASE_CLASS_I Fructose-bisphosphate aldolase class-I active site. VyLEGtLLKPN

Chain	Residue	Details
A	VAL221-ASN231

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Proton acceptor => ECO:0000250\|UniProtKB:P00883

Chain	Residue	Details
A	PRO188
B	PRO188
C	PRO188
D	PRO188
W	PRO188
X	PRO188
Y	PRO188
Z	PRO188

site_id	SWS_FT_FI2
Number of Residues	8
Details	ACT_SITE: Schiff-base intermediate with dihydroxyacetone-P => ECO:0000250\|UniProtKB:P00883

Chain	Residue	Details
A	PRO230
B	PRO230
C	PRO230
D	PRO230
W	PRO230
X	PRO230
Y	PRO230
Z	PRO230

site_id	SWS_FT_FI3
Number of Residues	24
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P00883

Chain	Residue	Details
A	LEU43
D	LEU43
D	GLY272
D	ALA304
W	LEU43
W	GLY272
W	ALA304
X	LEU43
X	GLY272
X	ALA304
Y	LEU43
A	GLY272
Y	GLY272
Y	ALA304
Z	LEU43
Z	GLY272
Z	ALA304
A	ALA304
B	LEU43
B	GLY272
B	ALA304
C	LEU43
C	GLY272
C	ALA304

site_id	SWS_FT_FI4
Number of Residues	8
Details	MOD_RES: N-acetylalanine => ECO:0000250\|UniProtKB:Q91Y97

Chain	Residue	Details
A	HIS2
B	HIS2
C	HIS2
D	HIS2
W	HIS2
X	HIS2
Y	HIS2
Z	HIS2

site_id	SWS_FT_FI5
Number of Residues	24
Details	MOD_RES: N6-succinyllysine => ECO:0000250\|UniProtKB:Q91Y97

Chain	Residue	Details
A	LYS13
D	LYS13
D	GLU121
D	ALA317
W	LYS13
W	GLU121
W	ALA317
X	LYS13
X	GLU121
X	ALA317
Y	LYS13
A	GLU121
Y	GLU121
Y	ALA317
Z	LYS13
Z	GLU121
Z	ALA317
A	ALA317
B	LYS13
B	GLU121
B	ALA317
C	LYS13
C	GLU121
C	ALA317

site_id	SWS_FT_FI6
Number of Residues	48
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:24275569

Chain	Residue	Details
A	VAL36
B	GLY272
B	GLU276
B	ALA309
C	VAL36
C	GLN89
C	GLU132
C	GLY272
C	GLU276
C	ALA309
D	VAL36
A	GLN89
D	GLN89
D	GLU132
D	GLY272
D	GLU276
D	ALA309
W	VAL36
W	GLN89
W	GLU132
W	GLY272
W	GLU276
A	GLU132
W	ALA309
X	VAL36
X	GLN89
X	GLU132
X	GLY272
X	GLU276
X	ALA309
Y	VAL36
Y	GLN89
Y	GLU132
A	GLY272
Y	GLY272
Y	GLU276
Y	ALA309
Z	VAL36
Z	GLN89
Z	GLU132
Z	GLY272
Z	GLU276
Z	ALA309
A	GLU276
A	ALA309
B	VAL36
B	GLN89
B	GLU132

site_id	SWS_FT_FI7
Number of Residues	16
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:24275569

Chain	Residue	Details
A	MET39
W	ASN119
X	MET39
X	ASN119
Y	MET39
Y	ASN119
Z	MET39
Z	ASN119
A	ASN119
B	MET39
B	ASN119
C	MET39
C	ASN119
D	MET39
D	ASN119
W	MET39

site_id	SWS_FT_FI8
Number of Residues	16
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P00884

Chain	Residue	Details
A	PHE299
W	TYR301
X	PHE299
X	TYR301
Y	PHE299
Y	TYR301
Z	PHE299
Z	TYR301
A	TYR301
B	PHE299
B	TYR301
C	PHE299
C	TYR301
D	PHE299
D	TYR301
W	PHE299

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1ald

Chain	Residue	Details
A	GLU187
A	LYS229
A	ASP33

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1ald

Chain	Residue	Details
B	GLU187
B	LYS229
B	ASP33

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1ald

Chain	Residue	Details
C	GLU187
C	LYS229
C	ASP33

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1ald

Chain	Residue	Details
D	GLU187
D	LYS229
D	ASP33

site_id	CSA5
Number of Residues	3
Details	Annotated By Reference To The Literature 1ald

Chain	Residue	Details
W	GLU187
W	LYS229
W	ASP33

site_id	CSA6
Number of Residues	3
Details	Annotated By Reference To The Literature 1ald

Chain	Residue	Details
X	GLU187
X	LYS229
X	ASP33

site_id	CSA7
Number of Residues	3
Details	Annotated By Reference To The Literature 1ald

Chain	Residue	Details
Y	GLU187
Y	LYS229
Y	ASP33

site_id	CSA8
Number of Residues	3
Details	Annotated By Reference To The Literature 1ald

Chain	Residue	Details
Z	GLU187
Z	LYS229
Z	ASP33

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)