Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1WSJ

Crystal structure of E.coli RNase HI active site mutant (K87A/H124A)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003676	molecular_function	nucleic acid binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0006401	biological_process	RNA catabolic process
A	0016787	molecular_function	hydrolase activity
A	0043137	biological_process	DNA replication, removal of RNA primer
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0003676	molecular_function	nucleic acid binding
B	0004518	molecular_function	nuclease activity
B	0004519	molecular_function	endonuclease activity
B	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0006401	biological_process	RNA catabolic process
B	0016787	molecular_function	hydrolase activity
B	0043137	biological_process	DNA replication, removal of RNA primer
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0003676	molecular_function	nucleic acid binding
C	0004518	molecular_function	nuclease activity
C	0004519	molecular_function	endonuclease activity
C	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0006401	biological_process	RNA catabolic process
C	0016787	molecular_function	hydrolase activity
C	0043137	biological_process	DNA replication, removal of RNA primer
C	0046872	molecular_function	metal ion binding
D	0000287	molecular_function	magnesium ion binding
D	0003676	molecular_function	nucleic acid binding
D	0004518	molecular_function	nuclease activity
D	0004519	molecular_function	endonuclease activity
D	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0006401	biological_process	RNA catabolic process
D	0016787	molecular_function	hydrolase activity
D	0043137	biological_process	DNA replication, removal of RNA primer
D	0046872	molecular_function	metal ion binding
E	0000287	molecular_function	magnesium ion binding
E	0003676	molecular_function	nucleic acid binding
E	0004518	molecular_function	nuclease activity
E	0004519	molecular_function	endonuclease activity
E	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0006401	biological_process	RNA catabolic process
E	0016787	molecular_function	hydrolase activity
E	0043137	biological_process	DNA replication, removal of RNA primer
E	0046872	molecular_function	metal ion binding
F	0000287	molecular_function	magnesium ion binding
F	0003676	molecular_function	nucleic acid binding
F	0004518	molecular_function	nuclease activity
F	0004519	molecular_function	endonuclease activity
F	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0006401	biological_process	RNA catabolic process
F	0016787	molecular_function	hydrolase activity
F	0043137	biological_process	DNA replication, removal of RNA primer
F	0046872	molecular_function	metal ion binding
G	0000287	molecular_function	magnesium ion binding
G	0003676	molecular_function	nucleic acid binding
G	0004518	molecular_function	nuclease activity
G	0004519	molecular_function	endonuclease activity
G	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0006401	biological_process	RNA catabolic process
G	0016787	molecular_function	hydrolase activity
G	0043137	biological_process	DNA replication, removal of RNA primer
G	0046872	molecular_function	metal ion binding
H	0000287	molecular_function	magnesium ion binding
H	0003676	molecular_function	nucleic acid binding
H	0004518	molecular_function	nuclease activity
H	0004519	molecular_function	endonuclease activity
H	0004523	molecular_function	RNA-DNA hybrid ribonuclease activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0006401	biological_process	RNA catabolic process
H	0016787	molecular_function	hydrolase activity
H	0043137	biological_process	DNA replication, removal of RNA primer
H	0046872	molecular_function	metal ion binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	423
Details	Domain: {"description":"RNase H type-1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00408","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	32
Details	Binding site: {"evidences":[{"source":"PubMed","id":"1311386","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 163

Chain	Residue	Details
A	ASP10	metal ligand
A	GLY11	metal ligand
A	GLU48	increase acidity, increase basicity, unknown
A	ASP70	metal ligand
A	ALA124	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, unknown
A	ASP134	increase acidity, metal ligand, proton acceptor, proton donor

site_id	MCSA2
Number of Residues	6
Details	M-CSA 163

Chain	Residue	Details
B	ASP10	metal ligand
B	GLY11	metal ligand
B	GLU48	increase acidity, increase basicity, unknown
B	ASP70	metal ligand
B	ALA124	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, unknown
B	ASP134	increase acidity, metal ligand, proton acceptor, proton donor

site_id	MCSA3
Number of Residues	6
Details	M-CSA 163

Chain	Residue	Details
C	ASP10	metal ligand
C	GLY11	metal ligand
C	GLU48	increase acidity, increase basicity, unknown
C	ASP70	metal ligand
C	ALA124	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, unknown
C	ASP134	increase acidity, metal ligand, proton acceptor, proton donor

site_id	MCSA4
Number of Residues	5
Details	M-CSA 163

Chain	Residue	Details
D	ASP10	metal ligand
D	GLY11	metal ligand
D	GLU48	increase acidity, increase basicity, unknown
D	ASP70	metal ligand
D	ASP134	increase acidity, metal ligand, proton acceptor, proton donor

site_id	MCSA5
Number of Residues	6
Details	M-CSA 163

Chain	Residue	Details
E	ASP10	metal ligand
E	GLY11	metal ligand
E	GLU48	increase acidity, increase basicity, unknown
E	ASP70	metal ligand
E	ALA124	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, unknown
E	ASP134	increase acidity, metal ligand, proton acceptor, proton donor

site_id	MCSA6
Number of Residues	5
Details	M-CSA 163

Chain	Residue	Details
F	ASP10	metal ligand
F	GLY11	metal ligand
F	GLU48	increase acidity, increase basicity, unknown
F	ASP70	metal ligand
F	ASP134	increase acidity, metal ligand, proton acceptor, proton donor

site_id	MCSA7
Number of Residues	5
Details	M-CSA 163

Chain	Residue	Details
G	ASP10	metal ligand
G	GLY11	metal ligand
G	GLU48	increase acidity, increase basicity, unknown
G	ASP70	metal ligand
G	ASP134	increase acidity, metal ligand, proton acceptor, proton donor

site_id	MCSA8
Number of Residues	5
Details	M-CSA 163

Chain	Residue	Details
H	ASP10	metal ligand
H	GLY11	metal ligand
H	GLU48	increase acidity, increase basicity, unknown
H	ASP70	metal ligand
H	ASP134	increase acidity, metal ligand, proton acceptor, proton donor

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)