1WDK
fatty acid beta-oxidation multienzyme complex from Pseudomonas fragi, form I (native2)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0003857 | molecular_function | 3-hydroxyacyl-CoA dehydrogenase (NAD+) activity |
| A | 0004165 | molecular_function | delta(3)-delta(2)-enoyl-CoA isomerase activity |
| A | 0004300 | molecular_function | enoyl-CoA hydratase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0006629 | biological_process | lipid metabolic process |
| A | 0006631 | biological_process | fatty acid metabolic process |
| A | 0006635 | biological_process | fatty acid beta-oxidation |
| A | 0008692 | molecular_function | 3-hydroxybutyryl-CoA epimerase activity |
| A | 0009062 | biological_process | fatty acid catabolic process |
| A | 0016042 | biological_process | lipid catabolic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016509 | molecular_function | long-chain-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016853 | molecular_function | isomerase activity |
| A | 0018812 | molecular_function | 3-hydroxyacyl-CoA dehydratase activity |
| A | 0036125 | cellular_component | fatty acid beta-oxidation multienzyme complex |
| A | 0070403 | molecular_function | NAD+ binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0003857 | molecular_function | 3-hydroxyacyl-CoA dehydrogenase (NAD+) activity |
| B | 0004165 | molecular_function | delta(3)-delta(2)-enoyl-CoA isomerase activity |
| B | 0004300 | molecular_function | enoyl-CoA hydratase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0006629 | biological_process | lipid metabolic process |
| B | 0006631 | biological_process | fatty acid metabolic process |
| B | 0006635 | biological_process | fatty acid beta-oxidation |
| B | 0008692 | molecular_function | 3-hydroxybutyryl-CoA epimerase activity |
| B | 0009062 | biological_process | fatty acid catabolic process |
| B | 0016042 | biological_process | lipid catabolic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016509 | molecular_function | long-chain-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0016829 | molecular_function | lyase activity |
| B | 0016853 | molecular_function | isomerase activity |
| B | 0018812 | molecular_function | 3-hydroxyacyl-CoA dehydratase activity |
| B | 0036125 | cellular_component | fatty acid beta-oxidation multienzyme complex |
| B | 0070403 | molecular_function | NAD+ binding |
| C | 0003988 | molecular_function | acetyl-CoA C-acyltransferase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006629 | biological_process | lipid metabolic process |
| C | 0006631 | biological_process | fatty acid metabolic process |
| C | 0006635 | biological_process | fatty acid beta-oxidation |
| C | 0010124 | biological_process | phenylacetate catabolic process |
| C | 0016042 | biological_process | lipid catabolic process |
| C | 0016740 | molecular_function | transferase activity |
| C | 0016746 | molecular_function | acyltransferase activity |
| C | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| C | 0044281 | biological_process | small molecule metabolic process |
| D | 0003988 | molecular_function | acetyl-CoA C-acyltransferase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006629 | biological_process | lipid metabolic process |
| D | 0006631 | biological_process | fatty acid metabolic process |
| D | 0006635 | biological_process | fatty acid beta-oxidation |
| D | 0010124 | biological_process | phenylacetate catabolic process |
| D | 0016042 | biological_process | lipid catabolic process |
| D | 0016740 | molecular_function | transferase activity |
| D | 0016746 | molecular_function | acyltransferase activity |
| D | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| D | 0044281 | biological_process | small molecule metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE HG D 1 |
| Chain | Residue |
| D | CYS95 |
| D | MET151 |
| D | PHE349 |
| D | ACO4001 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE HG C 392 |
| Chain | Residue |
| C | CYS95 |
| C | MET151 |
| C | PHE349 |
| C | ACO3001 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 716 |
| Chain | Residue |
| A | ASP573 |
| D | GLU266 |
| D | VAL391 |
| A | HIS550 |
| site_id | AC4 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE ACO C 3001 |
| Chain | Residue |
| C | CYS95 |
| C | MET130 |
| C | MET151 |
| C | HIS177 |
| C | ARG215 |
| C | THR218 |
| C | LEU223 |
| C | PHE230 |
| C | ALA239 |
| C | GLY240 |
| C | SER243 |
| C | ILE245 |
| C | MET284 |
| C | ALA314 |
| C | PHE315 |
| C | HIS347 |
| C | CYS377 |
| C | HG392 |
| C | HOH3206 |
| site_id | AC5 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE ACO D 4001 |
| Chain | Residue |
| D | HG1 |
| D | CYS95 |
| D | MET130 |
| D | MET151 |
| D | HIS177 |
| D | ARG215 |
| D | THR218 |
| D | LEU223 |
| D | ALA239 |
| D | GLY240 |
| D | SER243 |
| D | MET284 |
| D | ASN312 |
| D | ALA314 |
| D | PHE315 |
| D | HIS347 |
| D | CYS377 |
| D | GLY379 |
| D | HOH4152 |
| D | HOH4155 |
| D | HOH4169 |
| D | HOH4174 |
| site_id | AC6 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE NAD A 1001 |
| Chain | Residue |
| A | GLY321 |
| A | ALA322 |
| A | ILE324 |
| A | MET325 |
| A | ASP344 |
| A | ILE345 |
| A | ASN346 |
| A | GLY349 |
| A | VAL401 |
| A | GLU403 |
| A | LYS408 |
| A | VAL411 |
| A | ASN428 |
| A | THR429 |
| A | SER430 |
| A | PHE452 |
| A | ASN454 |
| A | HOH1087 |
| A | HOH1177 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE N8E A 1002 |
| Chain | Residue |
| A | MET459 |
| A | ASN501 |
| A | VAL659 |
| A | TYR660 |
| A | GLY661 |
| A | ILE662 |
| site_id | AC8 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE N8E A 1003 |
| Chain | Residue |
| A | GLY68 |
| A | ALA69 |
| A | ASP70 |
| A | ILE71 |
| A | PHE74 |
| A | GLY116 |
| A | GLY117 |
| A | PRO139 |
| A | GLU140 |
| A | LEU143 |
| A | GLN298 |
| A | LYS305 |
| site_id | AC9 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE NAD B 2001 |
| Chain | Residue |
| B | VAL411 |
| B | ASN428 |
| B | THR429 |
| B | SER430 |
| B | ASN454 |
| B | HOH2046 |
| B | HOH2288 |
| B | HOH2312 |
| B | GLY321 |
| B | ALA322 |
| B | ILE324 |
| B | MET325 |
| B | ASP344 |
| B | ILE345 |
| B | VAL401 |
| B | GLU403 |
| B | LYS408 |
| site_id | BC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE N8E B 2002 |
| Chain | Residue |
| B | MET459 |
| B | PRO460 |
| B | ASN501 |
| B | MET535 |
| B | VAL659 |
| B | TYR660 |
| B | GLY661 |
| B | GLY663 |
| site_id | BC2 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE N8E B 2003 |
| Chain | Residue |
| B | ARG34 |
| B | LEU37 |
| B | GLU73 |
| B | ASN77 |
| B | LEU80 |
| B | GLY88 |
| B | GLU120 |
| B | GLU140 |
| B | GLY148 |
| B | PHE149 |
| B | HOH2309 |
Functional Information from PROSITE/UniProt
| site_id | PS00067 |
| Number of Residues | 25 |
| Details | 3HCDH 3-hydroxyacyl-CoA dehydrogenase signature. DcpGFLvNRvlfPYFggfak.LVsaG |
| Chain | Residue | Details |
| A | ASP494-GLY518 |
| site_id | PS00098 |
| Number of Residues | 19 |
| Details | THIOLASE_1 Thiolases acyl-enzyme intermediate signature. VSRlCGSSMsALhtaaqaI |
| Chain | Residue | Details |
| C | VAL91-ILE109 |
| site_id | PS00099 |
| Number of Residues | 14 |
| Details | THIOLASE_3 Thiolases active site. GLSTMCIGlGqGiA |
| Chain | Residue | Details |
| C | GLY372-ALA385 |
| site_id | PS00166 |
| Number of Residues | 21 |
| Details | ENOYL_COA_HYDRATASE Enoyl-CoA hydratase/isomerase signature. VAaINGialGGGlemcLaADF |
| Chain | Residue | Details |
| A | VAL107-PHE127 |
| site_id | PS00178 |
| Number of Residues | 11 |
| Details | AA_TRNA_LIGASE_I Aminoacyl-transfer RNA synthetases class-I signature. Pk.GGtVTAGTS |
| Chain | Residue | Details |
| C | PRO232-SER242 |
| site_id | PS00737 |
| Number of Residues | 17 |
| Details | THIOLASE_2 Thiolases signature 2. NlhGGaIAlGHPfGcSG |
| Chain | Residue | Details |
| C | ASN337-GLY353 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 378 |
| Details | Region: {"description":"Enoyl-CoA hydratase/isomerase","evidences":[{"source":"HAMAP-Rule","id":"MF_01621","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Active site: {"description":"For 3-hydroxyacyl-CoA dehydrogenase activity","evidences":[{"source":"HAMAP-Rule","id":"MF_01621","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 20 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"15229654","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | Site: {"description":"Important for catalytic activity","evidences":[{"source":"HAMAP-Rule","id":"MF_01621","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Acyl-thioester intermediate","evidences":[{"source":"HAMAP-Rule","id":"MF_01620","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_01620","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| C | HIS347 | |
| C | CYS377 | |
| C | CYS95 | |
| C | GLY379 |
| site_id | CSA10 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| B | GLU140 | |
| B | GLY148 |
| site_id | CSA11 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| A | GLU120 | |
| A | GLU140 | |
| A | GLY117 |
| site_id | CSA12 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| B | GLU120 | |
| B | GLU140 | |
| B | GLY117 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| D | HIS347 | |
| D | CYS377 | |
| D | CYS95 | |
| D | GLY379 |
| site_id | CSA3 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| A | GLU120 | |
| A | GLU140 | |
| A | GLY117 | |
| A | ALA69 |
| site_id | CSA4 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| B | GLU120 | |
| B | GLU140 | |
| B | GLY117 | |
| B | ALA69 |
| site_id | CSA5 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| C | HIS347 | |
| C | CYS377 |
| site_id | CSA6 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| D | HIS347 | |
| D | CYS377 |
| site_id | CSA7 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| A | HIS451 | |
| A | SER430 | |
| A | ASN501 |
| site_id | CSA8 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| B | HIS451 | |
| B | SER430 | |
| B | ASN501 |
| site_id | CSA9 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1afw |
| Chain | Residue | Details |
| A | GLU140 | |
| A | GLY148 |
