1W8N

Contribution of the Active Site Aspartic Acid to Catalysis in the Bacterial Neuraminidase from Micromonospora viridifaciens.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004308	molecular_function	exo-alpha-sialidase activity
A	0005576	cellular_component	extracellular region
A	0005737	cellular_component	cytoplasm
A	0005975	biological_process	carbohydrate metabolic process
A	0006689	biological_process	ganglioside catabolic process
A	0008152	biological_process	metabolic process
A	0009313	biological_process	oligosaccharide catabolic process
A	0016020	cellular_component	membrane
A	0016787	molecular_function	hydrolase activity
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0043231	cellular_component	intracellular membrane-bounded organelle
A	0052794	molecular_function	exo-alpha-(2->3)-sialidase activity
A	0052795	molecular_function	exo-alpha-(2->6)-sialidase activity
A	0052796	molecular_function	exo-alpha-(2->8)-sialidase activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000250

Chain	Residue	Details
A	GLY92

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000255

Chain	Residue	Details
A	GLU260

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site_id	SWS_FT_FI3
Number of Residues	1
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
A	TYR370

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site_id	SWS_FT_FI4
Number of Residues	2
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	ARG68
A	ARG276

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 835

Chain	Residue	Details
A	GLY92	activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor
A	GLU260	activator, increase nucleophilicity, promote heterolysis, proton acceptor, proton donor
A	TYR370	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor

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