1W21
Structure of Neuraminidase from English duck subtype N6 complexed with 30 mM sialic acid (NANA, Neu5Ac), crystal soaked for 43 hours at 291 K.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004308 | molecular_function | exo-alpha-sialidase activity |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0016020 | cellular_component | membrane |
| A | 0033644 | cellular_component | host cell membrane |
| A | 0055036 | cellular_component | virion membrane |
| B | 0004308 | molecular_function | exo-alpha-sialidase activity |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0016020 | cellular_component | membrane |
| B | 0033644 | cellular_component | host cell membrane |
| B | 0055036 | cellular_component | virion membrane |
| C | 0004308 | molecular_function | exo-alpha-sialidase activity |
| C | 0005975 | biological_process | carbohydrate metabolic process |
| C | 0016020 | cellular_component | membrane |
| C | 0033644 | cellular_component | host cell membrane |
| C | 0055036 | cellular_component | virion membrane |
| D | 0004308 | molecular_function | exo-alpha-sialidase activity |
| D | 0005975 | biological_process | carbohydrate metabolic process |
| D | 0016020 | cellular_component | membrane |
| D | 0033644 | cellular_component | host cell membrane |
| D | 0055036 | cellular_component | virion membrane |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1516 |
| Details | Region: {"description":"Head of neuraminidase","evidences":[{"source":"HAMAP-Rule","id":"MF_04071","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"HAMAP-Rule","id":"MF_04071","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"HAMAP-Rule","id":"MF_04071","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 20 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_04071","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 12 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_04071","evidenceCode":"ECO:0000255"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"APR-2004","submissionDatabase":"PDB data bank","title":"The crystal structure of influenza type A virus neuraminidase of the N6 subtype at 1.85 A resolution.","authors":["Rudino-Pinera E.","Crennell S.J.","Webster R.G.","Laver W.G.","Garman E.F."]}},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAY-2006","submissionDatabase":"PDB data bank","title":"The crystal structure of type A influenza virus neuraminidase of the N6 subtype reveals the existence of two separate Neu5Ac binding sites.","authors":["Rudino-Pinera E.","Tunnah P.","Crennell S.J.","Webster R.G.","Laver W.G.","Garman E.F."]}}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"APR-2004","submissionDatabase":"PDB data bank","title":"The crystal structure of influenza type A virus neuraminidase of the N6 subtype at 1.85 A resolution.","authors":["Rudino-Pinera E.","Crennell S.J.","Webster R.G.","Laver W.G.","Garman E.F."]}},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAY-2006","submissionDatabase":"PDB data bank","title":"The crystal structure of type A influenza virus neuraminidase of the N6 subtype reveals the existence of two separate Neu5Ac binding sites.","authors":["Rudino-Pinera E.","Tunnah P.","Crennell S.J.","Webster R.G.","Laver W.G.","Garman E.F."]}}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 8 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine; by host","evidences":[{"source":"HAMAP-Rule","id":"MF_04071","evidenceCode":"ECO:0000255"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"APR-2004","submissionDatabase":"PDB data bank","title":"The crystal structure of influenza type A virus neuraminidase of the N6 subtype at 1.85 A resolution.","authors":["Rudino-Pinera E.","Crennell S.J.","Webster R.G.","Laver W.G.","Garman E.F."]}}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine; by host","evidences":[{"source":"HAMAP-Rule","id":"MF_04071","evidenceCode":"ECO:0000255"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"APR-2004","submissionDatabase":"PDB data bank","title":"The crystal structure of influenza type A virus neuraminidase of the N6 subtype at 1.85 A resolution.","authors":["Rudino-Pinera E.","Crennell S.J.","Webster R.G.","Laver W.G.","Garman E.F."]}},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"MAY-2006","submissionDatabase":"PDB data bank","title":"The crystal structure of type A influenza virus neuraminidase of the N6 subtype reveals the existence of two separate Neu5Ac binding sites.","authors":["Rudino-Pinera E.","Tunnah P.","Crennell S.J.","Webster R.G.","Laver W.G.","Garman E.F."]}}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 4 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine; by host","evidences":[{"source":"HAMAP-Rule","id":"MF_04071","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1a4g |
| Chain | Residue | Details |
| A | ASP157 | |
| A | GLU284 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1a4g |
| Chain | Residue | Details |
| B | ASP1157 | |
| B | GLU1284 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1a4g |
| Chain | Residue | Details |
| C | GLU2284 | |
| C | ASP2157 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1a4g |
| Chain | Residue | Details |
| D | ASP3157 | |
| D | GLU3284 |
