1VZZ

CRYSTAL STRUCTURE OF MUTANT ENZYME Y32F/D103L OF KETOSTEROID ISOMERASE FROM PSEUDOMONAS PUTIDA BIOTYPE B

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004769	molecular_function	steroid delta-isomerase activity
A	0008202	biological_process	steroid metabolic process
A	0016853	molecular_function	isomerase activity
B	0004769	molecular_function	steroid delta-isomerase activity
B	0008202	biological_process	steroid metabolic process
B	0016853	molecular_function	isomerase activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton donor

Chain	Residue	Details
A	TYR16
B	TYR16

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site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Proton acceptor

Chain	Residue	Details
A	ASP40
B	ASP40

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site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:11007792

Chain	Residue	Details
A	LEU103
B	LEU103

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	a catalytic site defined by CSA, PubMed 2706241, 9369474, 9103200, 10653633

Chain	Residue	Details
A	ASP40
A	TYR16
B	ASP103
A	ASP103

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site_id	CSA2
Number of Residues	2
Details	a catalytic site defined by CSA, PubMed 2706241, 9369474, 9103200, 10653633

Chain	Residue	Details
B	ASP40
B	TYR16

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site_id	MCSA1
Number of Residues	4
Details	M-CSA 349

Chain	Residue	Details
A	TYR16	proton acceptor, proton donor
A	ASP40	proton acceptor, proton donor
A	ASP100	electrostatic stabiliser
A	LEU103	electrostatic stabiliser

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site_id	MCSA2
Number of Residues	4
Details	M-CSA 349

Chain	Residue	Details
B	TYR16	proton acceptor, proton donor
B	ASP40	proton acceptor, proton donor
B	ASP100	electrostatic stabiliser
B	LEU103	electrostatic stabiliser

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