Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1VZT

ROLES OF INDIVIDUAL RESIDUES OF ALPHA-1,3 GALACTOSYLTRANSFERASES IN SUBSTRATE BINDING AND CATALYSIS

Replaces: 1O7R

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005975	biological_process	carbohydrate metabolic process
A	0016020	cellular_component	membrane
A	0016758	molecular_function	hexosyltransferase activity
B	0005975	biological_process	carbohydrate metabolic process
B	0016020	cellular_component	membrane
B	0016758	molecular_function	hexosyltransferase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN A 1369

Chain	Residue
A	ASP225
A	ASP227
A	UDP1370
A	HOH2211

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MN B 2369

Chain	Residue
B	ASP1225
B	ASP1227
B	HOH2221
B	UDP2370

site_id	AC3
Number of Residues	22
Details	BINDING SITE FOR RESIDUE UDP A 1370

Chain	Residue
A	ALA135
A	VAL136
A	TYR139
A	ILE198
A	SER199
A	ARG202
A	ASP225
A	VAL226
A	ASP227
A	LYS359
A	TYR361
A	ARG365
A	MN1369
A	TRS1371
A	HOH2110
A	HOH2211
A	HOH2212
A	HOH2213
A	HOH2214
A	HOH2215
A	HOH2216
A	PHE134

site_id	AC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE TRS A 1371

Chain	Residue
A	SER199
A	ALA281
A	TRP314
A	HIS315
A	ASP316
A	GLU317
A	UDP1370
A	HOH2214
A	HOH2215
A	HOH2217

site_id	AC5
Number of Residues	22
Details	BINDING SITE FOR RESIDUE UDP B 2370

Chain	Residue
B	PHE1134
B	ALA1135
B	VAL1136
B	TYR1139
B	ILE1198
B	SER1199
B	ARG1202
B	ASP1225
B	VAL1226
B	ASP1227
B	LYS1359
B	TYR1361
B	ARG1365
B	HOH2089
B	HOH2093
B	HOH2221
B	HOH2222
B	HOH2223
B	HOH2224
B	HOH2225
B	MN2369
B	TRS2371

site_id	AC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE TRS B 2371

Chain	Residue
B	ALA1281
B	TRP1314
B	HIS1315
B	ASP1316
B	GLU1317
B	HOH2090
B	HOH2223
B	HOH2225
B	UDP2370

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"11179209","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1G8O","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11179209","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11592969","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12011052","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1G93","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GWV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GWW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GX0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GX4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1K4V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1O7O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1O7Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1VZT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1VZU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1VZX","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JCK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VFZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VS3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VS4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VS5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VXL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WGZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11179209","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11592969","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12011052","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1G8O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1G93","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GWV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GWW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GX0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GX4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1K4V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1O7O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1O7Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1VZT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1VZU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1VZX","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JCK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VFZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VS3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VS4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VS5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VXL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WGZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12011052","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1GWV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GX4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1O7O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1O7Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1VZX","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VS4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WGZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11592969","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12011052","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1GWV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GWW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GX0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GX4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1K4V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1O7O","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1O7Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1VZT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1VZU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1VZX","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JCK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VFZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VS3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VS4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2VS5","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WGZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	2
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1g8o

Chain	Residue	Details
A	GLU317

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1g8o

Chain	Residue	Details
B	GLU1317

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1g8o

Chain	Residue	Details
A	GLU317
A	TRP314
A	ARG365

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1g8o

Chain	Residue	Details
B	GLU1317
B	ARG1365
B	TRP1314

site_id	MCSA1
Number of Residues	6
Details	M-CSA 356

Chain	Residue	Details
A	GLN247	electrostatic stabiliser, hydrogen bond donor
A	HIS280	electrostatic stabiliser, hydrogen bond donor
A	TRP314	electrostatic stabiliser, hydrogen bond donor
A	GLU317	activator, covalently attached, electrostatic stabiliser, nucleofuge, nucleophile
A	TRP356	electrostatic stabiliser, hydrogen bond donor
A	ARG365	electrostatic stabiliser

site_id	MCSA2
Number of Residues	6
Details	M-CSA 356

Chain	Residue	Details
B	GLN1247	electrostatic stabiliser, hydrogen bond donor
B	HIS1280	electrostatic stabiliser, hydrogen bond donor
B	TRP1314	electrostatic stabiliser, hydrogen bond donor
B	GLU1317	activator, covalently attached, electrostatic stabiliser, nucleofuge, nucleophile
B	TRP1356	electrostatic stabiliser, hydrogen bond donor
B	ARG1365	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)