1V9H

Crystal structure of the RNase MC1 mutant Y101A in complex with 5'-UMP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004521	molecular_function	RNA endonuclease activity
A	0005576	cellular_component	extracellular region
A	0006401	biological_process	RNA catabolic process
A	0016787	molecular_function	hydrolase activity
A	0016829	molecular_function	lyase activity
A	0033897	molecular_function	ribonuclease T2 activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 A 301

Chain	Residue
A	PRO52
A	PHE53
A	THR86
A	ARG132
A	GLY185
A	ALA186
A	HOH1030
A	HOH1043
A	HOH1068

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 302

Chain	Residue
A	GLY35
A	LEU36
A	TRP69
A	GLU84
A	TRP85
A	GLY89

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 303

Chain	Residue
A	ASN47
A	HIS83
A	LYS87
A	HOH1083

---

site_id	AC4
Number of Residues	15
Details	BINDING SITE FOR RESIDUE U5P A 1001

Chain	Residue
A	GLN9
A	HIS34
A	ASN71
A	VAL72
A	LEU73
A	ARG74
A	PHE80
A	HIS83
A	GLU84
A	LYS87
A	HIS88
A	HOH1039
A	HOH1050
A	HOH1055
A	HOH1077

---

Functional Information from PROSITE/UniProt

site_id	PS00530
Number of Residues	8
Details	RNASE_T2_1 Ribonuclease T2 family histidine active site 1. FtIHGLWP

Chain	Residue	Details
A	PHE31-PRO38

---

site_id	PS00531
Number of Residues	12
Details	RNASE_T2_2 Ribonuclease T2 family histidine active site 2. FwsHEWtKHGtC

Chain	Residue	Details
A	PHE80-CYS91

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Signal: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PROSITE-ProRule","id":"PRU10045","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12731868","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15322360","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"10446375","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1J1F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1J1G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UCD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1V9H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Active site: {"evidences":[{"source":"PubMed","id":"10964705","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12731868","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15322360","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"10446375","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1J1F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1J1G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UCA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UCD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1V9H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU10045","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12731868","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15322360","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"10446375","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1J1F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1J1G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UCD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1V9H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10964705","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"12731868","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"15322360","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1J1F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1J1G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UCA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UCC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UCD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1V9H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"12731868","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15322360","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1J1F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1J1G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UCD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1V9H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15322360","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12731868","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1J1F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1J1G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1V9H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15322360","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"10964705","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"12731868","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1J1G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UCA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UCD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1V9H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10964705","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"12731868","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"15322360","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"1J1F","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1J1G","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UCA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1UCD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1V9H","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	9
Details	Site: {"description":"Involved in thermostability","evidences":[{"source":"PubMed","id":"15322360","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1bol

Chain	Residue	Details
A	HIS34
A	GLU84
A	HIS88

---