Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1V5B

The Structure Of The Mutant, S225A and E251L, Of 3-Isopropylmalate Dehydrogenase From Bacillus Coagulans

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0009098	biological_process	L-leucine biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0046872	molecular_function	metal ion binding
A	0051287	molecular_function	NAD binding
B	0000287	molecular_function	magnesium ion binding
B	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0009098	biological_process	L-leucine biosynthetic process
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0046872	molecular_function	metal ion binding
B	0051287	molecular_function	NAD binding
C	0000287	molecular_function	magnesium ion binding
C	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0009098	biological_process	L-leucine biosynthetic process
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0046872	molecular_function	metal ion binding
C	0051287	molecular_function	NAD binding
D	0000287	molecular_function	magnesium ion binding
D	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0009098	biological_process	L-leucine biosynthetic process
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0046872	molecular_function	metal ion binding
D	0051287	molecular_function	NAD binding
E	0000287	molecular_function	magnesium ion binding
E	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0009098	biological_process	L-leucine biosynthetic process
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0046872	molecular_function	metal ion binding
E	0051287	molecular_function	NAD binding
F	0000287	molecular_function	magnesium ion binding
F	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0009098	biological_process	L-leucine biosynthetic process
F	0016491	molecular_function	oxidoreductase activity
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0046872	molecular_function	metal ion binding
F	0051287	molecular_function	NAD binding
G	0000287	molecular_function	magnesium ion binding
G	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0009098	biological_process	L-leucine biosynthetic process
G	0016491	molecular_function	oxidoreductase activity
G	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
G	0046872	molecular_function	metal ion binding
G	0051287	molecular_function	NAD binding
H	0000287	molecular_function	magnesium ion binding
H	0003862	molecular_function	3-isopropylmalate dehydrogenase activity
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0009098	biological_process	L-leucine biosynthetic process
H	0016491	molecular_function	oxidoreductase activity
H	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
H	0046872	molecular_function	metal ion binding
H	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 H 1001

Chain	Residue
E	LYS121
F	LYS121
F	ARG122
H	LYS319

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 H 1002

Chain	Residue
F	LYS319
G	LYS121
H	LYS121
H	ARG122

site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 H 1003

Chain	Residue
H	ARG134
H	ARG96

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 1004

Chain	Residue
B	LYS319
C	LYS121
C	ARG122
D	LYS121

site_id	AC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 1005

Chain	Residue
A	ARG350
G	ARG144

Functional Information from PROSITE/UniProt

site_id	PS00470
Number of Residues	20
Details	IDH_IMDH Isocitrate and isopropylmalate dehydrogenases signature. NMFGDIlSDlaSvit.GSLGM

Chain	Residue	Details
A	ASN242-MET261

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	64
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	GLY76
B	ARG96
B	ARG106
B	ARG134
B	ASP222
B	ASP246
B	ASP250
B	GLY280
C	GLY76
C	ARG96
C	ARG106
A	ARG96
C	ARG134
C	ASP222
C	ASP246
C	ASP250
C	GLY280
D	GLY76
D	ARG96
D	ARG106
D	ARG134
D	ASP222
A	ARG106
D	ASP246
D	ASP250
D	GLY280
E	GLY76
E	ARG96
E	ARG106
E	ARG134
E	ASP222
E	ASP246
E	ASP250
A	ARG134
E	GLY280
F	GLY76
F	ARG96
F	ARG106
F	ARG134
F	ASP222
F	ASP246
F	ASP250
F	GLY280
G	GLY76
A	ASP222
G	ARG96
G	ARG106
G	ARG134
G	ASP222
G	ASP246
G	ASP250
G	GLY280
H	GLY76
H	ARG96
H	ARG106
A	ASP246
H	ARG134
H	ASP222
H	ASP246
H	ASP250
H	GLY280
A	ASP250
A	GLY280
B	GLY76

site_id	SWS_FT_FI2
Number of Residues	16
Details	SITE: Important for catalysis => ECO:0000250

Chain	Residue	Details
A	TYR141
E	LYS190
F	TYR141
F	LYS190
G	TYR141
G	LYS190
H	TYR141
H	LYS190
A	LYS190
B	TYR141
B	LYS190
C	TYR141
C	LYS190
D	TYR141
D	LYS190
E	TYR141

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
A	ASP222
A	LYS190
B	TYR141

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
A	TYR141
B	ASP222
B	LYS190

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
D	TYR141
C	ASP222
C	LYS190

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
C	TYR141
D	ASP222
D	LYS190

site_id	CSA5
Number of Residues	3
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
F	TYR141
E	ASP222
E	LYS190

site_id	CSA6
Number of Residues	3
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
F	ASP222
F	LYS190
E	TYR141

site_id	CSA7
Number of Residues	3
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
H	TYR141
G	ASP222
G	LYS190

site_id	CSA8
Number of Residues	3
Details	Annotated By Reference To The Literature 1a05

Chain	Residue	Details
H	ASP222
H	LYS190
G	TYR141

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)