1V15

CRYSTAL STRUCTURE OF THE COLICIN E9, MUTANT HIS103ALA, IN COMPLEX WITH ZN+2 AND DSDNA (RESOLUTION 2.4A)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004519	molecular_function	endonuclease activity
A	0005102	molecular_function	signaling receptor binding
A	0009617	biological_process	response to bacterium
A	0019835	biological_process	cytolysis
B	0004519	molecular_function	endonuclease activity
B	0005102	molecular_function	signaling receptor binding
B	0009617	biological_process	response to bacterium
B	0019835	biological_process	cytolysis
C	0004519	molecular_function	endonuclease activity
C	0005102	molecular_function	signaling receptor binding
C	0009617	biological_process	response to bacterium
C	0019835	biological_process	cytolysis
D	0004519	molecular_function	endonuclease activity
D	0005102	molecular_function	signaling receptor binding
D	0009617	biological_process	response to bacterium
D	0019835	biological_process	cytolysis

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN A1132

Chain	Residue
A	HIS102
A	HIS127
A	HIS131
E	DT5
E	DC6

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN B1135

Chain	Residue
G	DC6
B	HIS102
B	HIS127
B	HIS131
G	DT5

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN C1132

Chain	Residue
C	HIS102
C	HIS127
C	HIS131
I	DT5
I	DC6

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN D1135

Chain	Residue
D	HIS102
D	HIS127
D	HIS131
K	DT5
K	DC6

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN E1009

Chain	Residue
E	DG3
E	HOH2004
L	DG9
L	DC10

---

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ZN G1009

Chain	Residue
G	DG3
G	HOH2009
J	DC10

---

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ZN I1009

Chain	Residue
H	DC10
I	DG3
I	HOH2006

---

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ZN K1009

Chain	Residue
F	DC10
K	DG3

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	44
Details	Compositional bias: {"description":"Basic and acidic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	96
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	39
Details	Compositional bias: {"description":"Low complexity","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	7
Details	M-CSA 791

Chain	Residue	Details
A	ARG5	electrostatic stabiliser
A	ARG96	electrostatic stabiliser
A	GLU100	electrostatic stabiliser
A	HIS102	metal ligand
A	ALA103	increase nucleophilicity, proton acceptor, proton donor
A	HIS127	metal ligand
A	HIS131	metal ligand

---

site_id	MCSA2
Number of Residues	7
Details	M-CSA 791

Chain	Residue	Details
B	ARG5	electrostatic stabiliser
B	ARG96	electrostatic stabiliser
B	GLU100	electrostatic stabiliser
B	HIS102	metal ligand
B	ALA103	increase nucleophilicity, proton acceptor, proton donor
B	HIS127	metal ligand
B	HIS131	metal ligand

---

site_id	MCSA3
Number of Residues	7
Details	M-CSA 791

Chain	Residue	Details
C	ARG5	electrostatic stabiliser
C	ARG96	electrostatic stabiliser
C	GLU100	electrostatic stabiliser
C	HIS102	metal ligand
C	ALA103	increase nucleophilicity, proton acceptor, proton donor
C	HIS127	metal ligand
C	HIS131	metal ligand

---

site_id	MCSA4
Number of Residues	7
Details	M-CSA 791

Chain	Residue	Details
D	ARG5	electrostatic stabiliser
D	ARG96	electrostatic stabiliser
D	GLU100	electrostatic stabiliser
D	HIS102	metal ligand
D	ALA103	increase nucleophilicity, proton acceptor, proton donor
D	HIS127	metal ligand
D	HIS131	metal ligand

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