1UXU

Structural basis for allosteric regulation and substrate specificity of the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) from Thermoproteus tenax

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0008886	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (non-phosphorylating) activity
A	0008911	molecular_function	lactaldehyde dehydrogenase (NAD+) activity
A	0016491	molecular_function	oxidoreductase activity
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0050661	molecular_function	NADP binding
A	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA A 754

Chain	Residue
A	GLU249
A	GLY255
A	HOH2147
A	HOH2148
A	HOH2153
A	HOH2159

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site_id	AC2
Number of Residues	21
Details	BINDING SITE FOR RESIDUE NAP A 502

Chain	Residue
A	PHE167
A	LYS191
A	SER193
A	ILE194
A	PRO223
A	GLY224
A	GLU228
A	PHE241
A	GLY243
A	SER244
A	VAL247
A	GLU395
A	PHE397
A	HOH2275
A	HOH2277
A	HOH2278
A	HOH2279
A	HOH2285
A	ILE164
A	THR165
A	PRO166

---

site_id	AC3
Number of Residues	15
Details	BINDING SITE FOR RESIDUE AMP A 503

Chain	Residue
A	ARG72
A	ARG79
A	ILE133
A	GLY134
A	GLY135
A	PRO139
A	ASP141
A	TRP142
A	ARG154
A	ARG155
A	ARG440
A	TRP498
A	HOH2115
A	HOH2280
A	HOH2281

---

site_id	AC4
Number of Residues	12
Details	BINDING SITE FOR RESIDUE G3H A 504

Chain	Residue
A	ASN168
A	TYR169
A	ARG296
A	CYS297
A	ASP298
A	ARG454
A	HIS455
A	GLY456
A	HOH2245
A	HOH2283
A	HOH2285
A	HOH2286

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: ACT_SITE => ECO:0000305|PubMed:15288789

Chain	Residue	Details
A	GLU263
A	CYS297

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:15288789, ECO:0007744|PDB:1UXR

Chain	Residue	Details
A	ARG72
A	ARG79
A	TYR184

---

site_id	SWS_FT_FI3
Number of Residues	5
Details	BINDING: BINDING => ECO:0000269|PubMed:15288789, ECO:0007744|PDB:1UXU

Chain	Residue	Details
A	GLY134
A	ARG154
A	ASN168
A	ARG296
A	HIS455

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:11842090, ECO:0000269|PubMed:15288789, ECO:0007744|PDB:1KY8, ECO:0007744|PDB:1UXN, ECO:0007744|PDB:1UXP, ECO:0007744|PDB:1UXQ, ECO:0007744|PDB:1UXR, ECO:0007744|PDB:1UXU, ECO:0007744|PDB:1UXV

Chain	Residue	Details
A	THR165
A	GLY224
A	GLU228
A	SER244

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:15288789, ECO:0007744|PDB:1UXT

Chain	Residue	Details
A	LYS191

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:11842090, ECO:0000269|PubMed:15288789, ECO:0007744|PDB:1KY8, ECO:0007744|PDB:1UXN, ECO:0007744|PDB:1UXP, ECO:0007744|PDB:1UXQ, ECO:0007744|PDB:1UXR, ECO:0007744|PDB:1UXV

Chain	Residue	Details
A	GLY265
A	GLU395

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1a4s

Chain	Residue	Details
A	CYS297
A	GLU263
A	ASN168

---