1U75

Electron Transfer Complex between Horse Heart Cytochrome c and Zinc-Porphyrin Substituted Cytochrome c Peroxidase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004601	molecular_function	peroxidase activity
A	0006979	biological_process	response to oxidative stress
A	0020037	molecular_function	heme binding
A	0034599	biological_process	cellular response to oxidative stress
B	0005515	molecular_function	protein binding
B	0005739	cellular_component	mitochondrion
B	0005758	cellular_component	mitochondrial intermembrane space
B	0005829	cellular_component	cytosol
B	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
B	0006123	biological_process	mitochondrial electron transport, cytochrome c to oxygen
B	0006915	biological_process	apoptotic process
B	0008289	molecular_function	lipid binding
B	0009055	molecular_function	electron transfer activity
B	0018063	biological_process	cytochrome c-heme linkage
B	0020037	molecular_function	heme binding
B	0042802	molecular_function	identical protein binding
B	0043065	biological_process	positive regulation of apoptotic process
B	0043280	biological_process	positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
B	0046872	molecular_function	metal ion binding
B	0070069	cellular_component	cytochrome complex
B	0070469	cellular_component	respirasome
B	2001056	biological_process	positive regulation of cysteine-type endopeptidase activity
C	0004601	molecular_function	peroxidase activity
C	0006979	biological_process	response to oxidative stress
C	0020037	molecular_function	heme binding
C	0034599	biological_process	cellular response to oxidative stress

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 A 2201

Chain	Residue
A	ARG48
A	TRP51
A	HIS52
A	HIS175
A	ZNH1001
A	HOH2004

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 C 2202

Chain	Residue
C	HIS175
C	ZNH1201
C	ARG48
C	TRP51
C	HIS52

---

site_id	AC3
Number of Residues	20
Details	BINDING SITE FOR RESIDUE ZNH A 1001

Chain	Residue
A	PRO44
A	ARG48
A	TRP51
A	LEU171
A	MET172
A	ALA174
A	HIS175
A	LEU177
A	GLY178
A	LYS179
A	THR180
A	HIS181
A	ASN184
A	SER185
A	TRP191
A	LEU232
A	THR234
A	PHE266
A	HOH2004
A	PO42201

---

site_id	AC4
Number of Residues	17
Details	BINDING SITE FOR RESIDUE HEC B 1101

Chain	Residue
B	LYS13
B	CYS14
B	CYS17
B	HIS18
B	THR28
B	THR40
B	GLY41
B	GLN42
B	TYR48
B	THR49
B	ASN52
B	TRP59
B	TYR67
B	THR78
B	LYS79
B	MET80
B	PHE82

---

site_id	AC5
Number of Residues	17
Details	BINDING SITE FOR RESIDUE ZNH C 1201

Chain	Residue
C	PRO44
C	ARG48
C	TRP51
C	PRO145
C	ASP146
C	LEU171
C	ALA174
C	HIS175
C	GLY178
C	LYS179
C	HIS181
C	ASN184
C	SER185
C	TRP191
C	HOH1422
C	HOH1534
C	PO42202

---

Functional Information from PROSITE/UniProt

site_id	PS00435
Number of Residues	11
Details	PEROXIDASE_1 Peroxidases proximal heme-ligand signature. EVVALMGAHAL

Chain	Residue	Details
A	GLU167-LEU177

---

site_id	PS00436
Number of Residues	12
Details	PEROXIDASE_2 Peroxidases active site signature. GPvlVRLaWHIS

Chain	Residue	Details
A	GLY43-SER54

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton acceptor

Chain	Residue	Details
A	HIS52
C	HIS52

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Tryptophan radical intermediate => ECO:0000269|PubMed:2851317

Chain	Residue	Details
A	TRP191
C	TRP191

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: axial binding residue => ECO:0000269|PubMed:10722697, ECO:0000269|PubMed:11170452, ECO:0000269|PubMed:2169873, ECO:0000269|PubMed:6092361, ECO:0000269|PubMed:8384877, ECO:0000269|PubMed:8673607

Chain	Residue	Details
A	HIS175
C	HIS175

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: Transition state stabilizer

Chain	Residue	Details
A	ARG48
C	ARG48

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18407956

Chain	Residue	Details
A	TYR153
C	TYR153

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:P62897

Chain	Residue	Details
B	GLY56

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P62897

Chain	Residue	Details
B	LYS73

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P62897

Chain	Residue	Details
B	LYS100

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1apx

Chain	Residue	Details
A	ARG48
A	HIS52
A	ASN82

---

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1apx

Chain	Residue	Details
C	ARG48
C	HIS52
C	ASN82

---

site_id	MCSA1
Number of Residues	3
Details	M-CSA 709

Chain	Residue	Details
A	ARG48	electrostatic stabiliser
A	HIS52	electrostatic stabiliser, proton acceptor, proton donor
A	TRP191	single electron acceptor, single electron donor

---

site_id	MCSA2
Number of Residues	3
Details	M-CSA 709

Chain	Residue	Details
C	ARG48	electrostatic stabiliser
C	HIS52	electrostatic stabiliser, proton acceptor, proton donor
C	TRP191	single electron acceptor, single electron donor

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