1U3T
Crystal Structure of Human Alcohol Dehydrogenase Alpha-Alpha Isoform Complexed with N-Cyclopentyl-N-Cyclobutylformamide Determined to 2.5 Angstrom Resolution
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004022 | molecular_function | alcohol dehydrogenase (NAD+) activity |
| A | 0004745 | molecular_function | all-trans-retinol dehydrogenase (NAD+) activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006066 | biological_process | alcohol metabolic process |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0042572 | biological_process | retinol metabolic process |
| A | 0042573 | biological_process | retinoic acid metabolic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 1990362 | molecular_function | butanol dehydrogenase (NAD+) activity |
| B | 0004022 | molecular_function | alcohol dehydrogenase (NAD+) activity |
| B | 0004745 | molecular_function | all-trans-retinol dehydrogenase (NAD+) activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006066 | biological_process | alcohol metabolic process |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0042572 | biological_process | retinol metabolic process |
| B | 0042573 | biological_process | retinoic acid metabolic process |
| B | 0046872 | molecular_function | metal ion binding |
| B | 1990362 | molecular_function | butanol dehydrogenase (NAD+) activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 375 |
| Chain | Residue |
| A | CYS97 |
| A | CYS100 |
| A | CYS103 |
| A | CYS111 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ZN A 376 |
| Chain | Residue |
| A | CYS46 |
| A | HIS67 |
| A | CYS174 |
| A | NAD1377 |
| A | CCB1378 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 375 |
| Chain | Residue |
| B | CYS97 |
| B | CYS100 |
| B | CYS103 |
| B | CYS111 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ZN B 376 |
| Chain | Residue |
| B | CYS46 |
| B | HIS67 |
| B | CYS174 |
| B | NAD2377 |
| B | CCB2378 |
| site_id | AC5 |
| Number of Residues | 33 |
| Details | BINDING SITE FOR RESIDUE NAD A 1377 |
| Chain | Residue |
| A | GLY47 |
| A | THR48 |
| A | HIS51 |
| A | CYS174 |
| A | THR178 |
| A | GLY199 |
| A | LEU200 |
| A | GLY201 |
| A | GLY202 |
| A | VAL203 |
| A | ASP223 |
| A | ILE224 |
| A | LYS228 |
| A | VAL268 |
| A | ILE269 |
| A | VAL292 |
| A | GLY293 |
| A | VAL294 |
| A | ALA317 |
| A | ILE318 |
| A | LEU319 |
| A | LEU362 |
| A | ARG369 |
| A | ZN376 |
| A | CCB1378 |
| A | HOH1501 |
| A | HOH1511 |
| A | HOH1514 |
| A | HOH1527 |
| A | HOH1530 |
| A | HOH1534 |
| A | HOH1549 |
| A | HOH1551 |
| site_id | AC6 |
| Number of Residues | 31 |
| Details | BINDING SITE FOR RESIDUE NAD B 2377 |
| Chain | Residue |
| B | GLY47 |
| B | THR48 |
| B | HIS51 |
| B | CYS174 |
| B | THR178 |
| B | GLY199 |
| B | LEU200 |
| B | GLY201 |
| B | GLY202 |
| B | VAL203 |
| B | ASP223 |
| B | ILE224 |
| B | LYS228 |
| B | ILE269 |
| B | ARG271 |
| B | VAL292 |
| B | GLY293 |
| B | VAL294 |
| B | ALA317 |
| B | ILE318 |
| B | LEU319 |
| B | LEU362 |
| B | ARG369 |
| B | ZN376 |
| B | CCB2378 |
| B | HOH2389 |
| B | HOH2415 |
| B | HOH2419 |
| B | HOH2442 |
| B | HOH2445 |
| B | HOH2516 |
| site_id | AC7 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE CCB A 1378 |
| Chain | Residue |
| A | THR48 |
| A | MET57 |
| A | HIS67 |
| A | ALA93 |
| A | ILE94 |
| A | VAL116 |
| A | CYS174 |
| A | VAL294 |
| A | ILE318 |
| A | LEU319 |
| A | ZN376 |
| A | NAD1377 |
| site_id | AC8 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE CCB B 2378 |
| Chain | Residue |
| B | VAL294 |
| B | ILE318 |
| B | ZN376 |
| B | NAD2377 |
| B | THR48 |
| B | HIS67 |
| B | ALA93 |
| B | ILE94 |
| B | CYS174 |
Functional Information from PROSITE/UniProt
| site_id | PS00059 |
| Number of Residues | 15 |
| Details | ADH_ZINC Zinc-containing alcohol dehydrogenases signature. GHEaAGIvesvGegV |
| Chain | Residue | Details |
| A | GLY66-VAL80 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 30 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11274460, ECO:0000269|PubMed:15449945, ECO:0007744|PDB:1HSO, ECO:0007744|PDB:1U3T |
| Chain | Residue | Details |
| A | GLY47 | |
| A | ILE224 | |
| A | PHE229 | |
| A | GLY270 | |
| A | GLY293 | |
| A | ILE318 | |
| A | THR370 | |
| B | GLY47 | |
| B | THR48 | |
| B | GLU68 | |
| B | GLY98 | |
| A | THR48 | |
| B | ARG101 | |
| B | LYS104 | |
| B | LEU112 | |
| B | GLY175 | |
| B | LEU200 | |
| B | ILE224 | |
| B | PHE229 | |
| B | GLY270 | |
| B | GLY293 | |
| B | ILE318 | |
| A | GLU68 | |
| B | THR370 | |
| A | GLY98 | |
| A | ARG101 | |
| A | LYS104 | |
| A | LEU112 | |
| A | GLY175 | |
| A | LEU200 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | MOD_RES: N-acetylserine => ECO:0000269|PubMed:3013304 |
| Chain | Residue | Details |
| A | THR2 | |
| B | THR2 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P00325 |
| Chain | Residue | Details |
| A | ILE23 | |
| B | ILE23 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1teh |
| Chain | Residue | Details |
| A | THR48 | |
| A | GLY47 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1teh |
| Chain | Residue | Details |
| B | THR48 | |
| B | GLY47 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1teh |
| Chain | Residue | Details |
| A | THR48 | |
| A | HIS51 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1teh |
| Chain | Residue | Details |
| B | THR48 | |
| B | HIS51 |
| site_id | CSA5 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1teh |
| Chain | Residue | Details |
| A | MET57 |
| site_id | CSA6 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1teh |
| Chain | Residue | Details |
| B | MET57 |
