1U2E

Crystal Structure of the C-C bond hydrolase MhpC

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0005737	cellular_component	cytoplasm
A	0009056	biological_process	catabolic process
A	0016787	molecular_function	hydrolase activity
A	0018771	molecular_function	2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity
A	0019380	biological_process	3-phenylpropionate catabolic process
A	0019622	biological_process	3-(3-hydroxy)phenylpropionate catabolic process
A	0042803	molecular_function	protein homodimerization activity
A	0052823	molecular_function	2-hydroxy-6-oxonona-2,4,7-trienedioate hydrolase activity
B	0003824	molecular_function	catalytic activity
B	0005737	cellular_component	cytoplasm
B	0009056	biological_process	catabolic process
B	0016787	molecular_function	hydrolase activity
B	0018771	molecular_function	2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity
B	0019380	biological_process	3-phenylpropionate catabolic process
B	0019622	biological_process	3-(3-hydroxy)phenylpropionate catabolic process
B	0042803	molecular_function	protein homodimerization activity
B	0052823	molecular_function	2-hydroxy-6-oxonona-2,4,7-trienedioate hydrolase activity
C	0003824	molecular_function	catalytic activity
C	0005737	cellular_component	cytoplasm
C	0009056	biological_process	catabolic process
C	0016787	molecular_function	hydrolase activity
C	0018771	molecular_function	2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity
C	0019380	biological_process	3-phenylpropionate catabolic process
C	0019622	biological_process	3-(3-hydroxy)phenylpropionate catabolic process
C	0042803	molecular_function	protein homodimerization activity
C	0052823	molecular_function	2-hydroxy-6-oxonona-2,4,7-trienedioate hydrolase activity
D	0003824	molecular_function	catalytic activity
D	0005737	cellular_component	cytoplasm
D	0009056	biological_process	catabolic process
D	0016787	molecular_function	hydrolase activity
D	0018771	molecular_function	2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity
D	0019380	biological_process	3-phenylpropionate catabolic process
D	0019622	biological_process	3-(3-hydroxy)phenylpropionate catabolic process
D	0042803	molecular_function	protein homodimerization activity
D	0052823	molecular_function	2-hydroxy-6-oxonona-2,4,7-trienedioate hydrolase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL D 1001

Chain	Residue
D	GLY3041
D	ALA3044
D	ASN3049
D	ARG3188

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site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL A 1002

Chain	Residue
A	GLY41
A	ALA44
A	ARG188

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CL C 1003

Chain	Residue
C	ALA2044
C	ARG2188
C	HOH215
C	GLY2041

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL B 1285

Chain	Residue
B	GLY1039
B	GLY1041
B	ASN1049

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_01654, ECO:0000269|PubMed:15663941

Chain	Residue	Details
A	HIS263
B	HIS1263
C	HIS2263
D	HIS3263

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	SITE: Transition state stabilizer => ECO:0000255|HAMAP-Rule:MF_01654, ECO:0000269|PubMed:15663941

Chain	Residue	Details
A	SER110
B	SER1110
C	SER2110
D	SER3110

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	SITE: Catalytic role in ketonization of the dienol substrate (substrate destabilization) => ECO:0000255|HAMAP-Rule:MF_01654, ECO:0000269|PubMed:15663941, ECO:0000269|PubMed:17029402

Chain	Residue	Details
A	ARG188
B	ARG1188
C	ARG2188
D	ARG3188

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1azw

Chain	Residue	Details
A	HIS263
A	SER110
A	ASP235

---

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1azw

Chain	Residue	Details
B	HIS1263
B	ASP1235
B	SER1110

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1azw

Chain	Residue	Details
C	ASP2235
C	SER2110
C	HIS2263

---

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1azw

Chain	Residue	Details
D	ASP3235
D	HIS3263
D	SER3110

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