Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1U1F

Structure of e. coli uridine phosphorylase complexed to 5-(m-(benzyloxy)benzyl)acyclouridine (BBAU)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004731	molecular_function	purine-nucleoside phosphorylase activity
A	0004850	molecular_function	uridine phosphorylase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006152	biological_process	purine nucleoside catabolic process
A	0006218	biological_process	uridine catabolic process
A	0006974	biological_process	DNA damage response
A	0009116	biological_process	nucleoside metabolic process
A	0009164	biological_process	nucleoside catabolic process
A	0009166	biological_process	nucleotide catabolic process
A	0016740	molecular_function	transferase activity
A	0016757	molecular_function	glycosyltransferase activity
A	0016763	molecular_function	pentosyltransferase activity
A	0030955	molecular_function	potassium ion binding
A	0032991	cellular_component	protein-containing complex
A	0042802	molecular_function	identical protein binding
A	0044206	biological_process	UMP salvage
B	0003824	molecular_function	catalytic activity
B	0004731	molecular_function	purine-nucleoside phosphorylase activity
B	0004850	molecular_function	uridine phosphorylase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006152	biological_process	purine nucleoside catabolic process
B	0006218	biological_process	uridine catabolic process
B	0006974	biological_process	DNA damage response
B	0009116	biological_process	nucleoside metabolic process
B	0009164	biological_process	nucleoside catabolic process
B	0009166	biological_process	nucleotide catabolic process
B	0016740	molecular_function	transferase activity
B	0016757	molecular_function	glycosyltransferase activity
B	0016763	molecular_function	pentosyltransferase activity
B	0030955	molecular_function	potassium ion binding
B	0032991	cellular_component	protein-containing complex
B	0042802	molecular_function	identical protein binding
B	0044206	biological_process	UMP salvage
C	0003824	molecular_function	catalytic activity
C	0004731	molecular_function	purine-nucleoside phosphorylase activity
C	0004850	molecular_function	uridine phosphorylase activity
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006152	biological_process	purine nucleoside catabolic process
C	0006218	biological_process	uridine catabolic process
C	0006974	biological_process	DNA damage response
C	0009116	biological_process	nucleoside metabolic process
C	0009164	biological_process	nucleoside catabolic process
C	0009166	biological_process	nucleotide catabolic process
C	0016740	molecular_function	transferase activity
C	0016757	molecular_function	glycosyltransferase activity
C	0016763	molecular_function	pentosyltransferase activity
C	0030955	molecular_function	potassium ion binding
C	0032991	cellular_component	protein-containing complex
C	0042802	molecular_function	identical protein binding
C	0044206	biological_process	UMP salvage
D	0003824	molecular_function	catalytic activity
D	0004731	molecular_function	purine-nucleoside phosphorylase activity
D	0004850	molecular_function	uridine phosphorylase activity
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006152	biological_process	purine nucleoside catabolic process
D	0006218	biological_process	uridine catabolic process
D	0006974	biological_process	DNA damage response
D	0009116	biological_process	nucleoside metabolic process
D	0009164	biological_process	nucleoside catabolic process
D	0009166	biological_process	nucleotide catabolic process
D	0016740	molecular_function	transferase activity
D	0016757	molecular_function	glycosyltransferase activity
D	0016763	molecular_function	pentosyltransferase activity
D	0030955	molecular_function	potassium ion binding
D	0032991	cellular_component	protein-containing complex
D	0042802	molecular_function	identical protein binding
D	0044206	biological_process	UMP salvage
E	0003824	molecular_function	catalytic activity
E	0004731	molecular_function	purine-nucleoside phosphorylase activity
E	0004850	molecular_function	uridine phosphorylase activity
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006152	biological_process	purine nucleoside catabolic process
E	0006218	biological_process	uridine catabolic process
E	0006974	biological_process	DNA damage response
E	0009116	biological_process	nucleoside metabolic process
E	0009164	biological_process	nucleoside catabolic process
E	0009166	biological_process	nucleotide catabolic process
E	0016740	molecular_function	transferase activity
E	0016757	molecular_function	glycosyltransferase activity
E	0016763	molecular_function	pentosyltransferase activity
E	0030955	molecular_function	potassium ion binding
E	0032991	cellular_component	protein-containing complex
E	0042802	molecular_function	identical protein binding
E	0044206	biological_process	UMP salvage
F	0003824	molecular_function	catalytic activity
F	0004731	molecular_function	purine-nucleoside phosphorylase activity
F	0004850	molecular_function	uridine phosphorylase activity
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006152	biological_process	purine nucleoside catabolic process
F	0006218	biological_process	uridine catabolic process
F	0006974	biological_process	DNA damage response
F	0009116	biological_process	nucleoside metabolic process
F	0009164	biological_process	nucleoside catabolic process
F	0009166	biological_process	nucleotide catabolic process
F	0016740	molecular_function	transferase activity
F	0016757	molecular_function	glycosyltransferase activity
F	0016763	molecular_function	pentosyltransferase activity
F	0030955	molecular_function	potassium ion binding
F	0032991	cellular_component	protein-containing complex
F	0042802	molecular_function	identical protein binding
F	0044206	biological_process	UMP salvage

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PO4 B 3301

Chain	Residue
A	ARG48
B	GLY26
B	ARG30
B	ARG91
B	ILE92
B	GLY93
B	THR94
B	GLU198
B	HOH4353

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 A 4301

Chain	Residue
A	GLY26
A	ARG30
A	ARG91
A	ILE92
A	GLY93
A	THR94
A	GLU198
A	HOH4356
A	HOH4404
B	ARG48

site_id	AC3
Number of Residues	11
Details	BINDING SITE FOR RESIDUE PO4 D 5301

Chain	Residue
C	ARG48
D	GLY26
D	ARG30
D	ARG91
D	ILE92
D	GLY93
D	THR94
D	GLU198
D	HOH6335
D	HOH6358
D	HOH6392

site_id	AC4
Number of Residues	11
Details	BINDING SITE FOR RESIDUE PO4 C 6301

Chain	Residue
C	GLY26
C	ARG30
C	ARG91
C	ILE92
C	GLY93
C	THR94
C	GLU198
C	1835300
C	HOH6375
C	HOH6420
D	ARG48

site_id	AC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 F 7301

Chain	Residue
E	ARG48
F	GLY26
F	ARG30
F	ARG91
F	ILE92
F	GLY93
F	THR94
F	GLU198
F	HOH8337
F	HOH8347

site_id	AC6
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 E 8301

Chain	Residue
E	GLY26
E	ARG30
E	ARG91
E	ILE92
E	GLY93
E	THR94
E	GLU198
E	1837300
E	HOH8356
F	ARG48

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE K C 1001

Chain	Residue
C	GLU49
C	ILE69
C	SER73
D	GLU49
D	ILE69
D	SER73

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE K A 1002

Chain	Residue
A	GLU49
A	ILE69
A	SER73
A	HOH4338
B	GLU49
B	ILE69
B	SER73

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE K E 1003

Chain	Residue
E	GLU49
E	ILE69
E	SER73
F	GLU49
F	ILE69
F	SER73

site_id	BC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE 183 A 3300

Chain	Residue
A	THR94
A	THR95
A	GLY96
A	PHE162
A	GLN166
A	ARG168
A	TYR195
A	GLU196
A	MET197
A	ILE220
A	MET234
A	HOH4324
A	HOH4372
B	PHE7
B	HIS8

site_id	BC2
Number of Residues	15
Details	BINDING SITE FOR RESIDUE 183 B 4300

Chain	Residue
B	GLY96
B	PHE162
B	GLN166
B	ARG168
B	TYR195
B	GLU196
B	MET197
B	ILE220
B	GLU227
B	HOH4356
B	HOH4381
A	PHE7
A	HIS8
A	ARG48
B	THR94

site_id	BC3
Number of Residues	18
Details	BINDING SITE FOR RESIDUE 183 C 5300

Chain	Residue
C	THR94
C	THR95
C	GLY96
C	PHE162
C	GLN166
C	ARG168
C	TYR195
C	GLU196
C	MET197
C	ILE220
C	PRO229
C	MET234
C	PO46301
C	HOH6308
C	HOH6375
C	HOH6377
D	PHE7
D	HIS8

site_id	BC4
Number of Residues	14
Details	BINDING SITE FOR RESIDUE 183 D 6300

Chain	Residue
C	PHE7
C	HIS8
D	THR94
D	THR95
D	GLY96
D	PHE162
D	GLN166
D	ARG168
D	TYR195
D	GLU196
D	MET197
D	ILE220
D	GLU227
D	HOH6327

site_id	BC5
Number of Residues	16
Details	BINDING SITE FOR RESIDUE 183 E 7300

Chain	Residue
E	THR94
E	THR95
E	GLY96
E	PHE162
E	GLN166
E	ARG168
E	TYR195
E	GLU196
E	MET197
E	ILE220
E	MET234
E	PO48301
E	HOH8329
E	HOH8344
F	PHE7
F	HIS8

site_id	BC6
Number of Residues	12
Details	BINDING SITE FOR RESIDUE 183 F 8300

Chain	Residue
E	PHE7
E	HIS8
F	THR94
F	GLY96
F	PHE162
F	GLN166
F	ARG168
F	TYR195
F	GLU196
F	MET197
F	VAL221
F	HOH8310

Functional Information from PROSITE/UniProt

site_id	PS01232
Number of Residues	16
Details	PNP_UDP_1 Purine and other phosphorylases family 1 signature. StGIGgPStSIaveEL

Chain	Residue	Details
A	SER66-LEU81

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 787

Chain	Residue	Details
A	THR224	electrostatic stabiliser, polar interaction
A	LEU9	proton acceptor, proton donor
A	LEU81	electrostatic stabiliser, polar interaction
A	TYR169	proton acceptor, proton donor

site_id	MCSA2
Number of Residues	4
Details	M-CSA 787

Chain	Residue	Details
B	LEU9	proton acceptor, proton donor
B	LEU81	electrostatic stabiliser, polar interaction
B	TYR169	proton acceptor, proton donor
B	THR224	electrostatic stabiliser, polar interaction

site_id	MCSA3
Number of Residues	4
Details	M-CSA 787

Chain	Residue	Details
C	LEU9	proton acceptor, proton donor
C	LEU81	electrostatic stabiliser, polar interaction
C	TYR169	proton acceptor, proton donor
C	THR224	electrostatic stabiliser, polar interaction

site_id	MCSA4
Number of Residues	4
Details	M-CSA 787

Chain	Residue	Details
D	LEU9	proton acceptor, proton donor
D	LEU81	electrostatic stabiliser, polar interaction
D	TYR169	proton acceptor, proton donor
D	THR224	electrostatic stabiliser, polar interaction

site_id	MCSA5
Number of Residues	4
Details	M-CSA 787

Chain	Residue	Details
E	THR224	electrostatic stabiliser, polar interaction
E	LEU9	proton acceptor, proton donor
E	LEU81	electrostatic stabiliser, polar interaction
E	TYR169	proton acceptor, proton donor

site_id	MCSA6
Number of Residues	4
Details	M-CSA 787

Chain	Residue	Details
F	LEU9	proton acceptor, proton donor
F	LEU81	electrostatic stabiliser, polar interaction
F	TYR169	proton acceptor, proton donor
F	THR224	electrostatic stabiliser, polar interaction

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)