Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1U1E

Structure of e. coli uridine phosphorylase complexed to 5(phenylseleno)acyclouridine (PSAU)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004731	molecular_function	purine-nucleoside phosphorylase activity
A	0004850	molecular_function	uridine phosphorylase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006152	biological_process	purine nucleoside catabolic process
A	0006218	biological_process	uridine catabolic process
A	0006974	biological_process	DNA damage response
A	0009116	biological_process	nucleoside metabolic process
A	0009164	biological_process	nucleoside catabolic process
A	0009166	biological_process	nucleotide catabolic process
A	0016740	molecular_function	transferase activity
A	0016757	molecular_function	glycosyltransferase activity
A	0016763	molecular_function	pentosyltransferase activity
A	0030955	molecular_function	potassium ion binding
A	0032991	cellular_component	protein-containing complex
A	0042802	molecular_function	identical protein binding
A	0044206	biological_process	UMP salvage
B	0003824	molecular_function	catalytic activity
B	0004731	molecular_function	purine-nucleoside phosphorylase activity
B	0004850	molecular_function	uridine phosphorylase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006152	biological_process	purine nucleoside catabolic process
B	0006218	biological_process	uridine catabolic process
B	0006974	biological_process	DNA damage response
B	0009116	biological_process	nucleoside metabolic process
B	0009164	biological_process	nucleoside catabolic process
B	0009166	biological_process	nucleotide catabolic process
B	0016740	molecular_function	transferase activity
B	0016757	molecular_function	glycosyltransferase activity
B	0016763	molecular_function	pentosyltransferase activity
B	0030955	molecular_function	potassium ion binding
B	0032991	cellular_component	protein-containing complex
B	0042802	molecular_function	identical protein binding
B	0044206	biological_process	UMP salvage
C	0003824	molecular_function	catalytic activity
C	0004731	molecular_function	purine-nucleoside phosphorylase activity
C	0004850	molecular_function	uridine phosphorylase activity
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006152	biological_process	purine nucleoside catabolic process
C	0006218	biological_process	uridine catabolic process
C	0006974	biological_process	DNA damage response
C	0009116	biological_process	nucleoside metabolic process
C	0009164	biological_process	nucleoside catabolic process
C	0009166	biological_process	nucleotide catabolic process
C	0016740	molecular_function	transferase activity
C	0016757	molecular_function	glycosyltransferase activity
C	0016763	molecular_function	pentosyltransferase activity
C	0030955	molecular_function	potassium ion binding
C	0032991	cellular_component	protein-containing complex
C	0042802	molecular_function	identical protein binding
C	0044206	biological_process	UMP salvage
D	0003824	molecular_function	catalytic activity
D	0004731	molecular_function	purine-nucleoside phosphorylase activity
D	0004850	molecular_function	uridine phosphorylase activity
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006152	biological_process	purine nucleoside catabolic process
D	0006218	biological_process	uridine catabolic process
D	0006974	biological_process	DNA damage response
D	0009116	biological_process	nucleoside metabolic process
D	0009164	biological_process	nucleoside catabolic process
D	0009166	biological_process	nucleotide catabolic process
D	0016740	molecular_function	transferase activity
D	0016757	molecular_function	glycosyltransferase activity
D	0016763	molecular_function	pentosyltransferase activity
D	0030955	molecular_function	potassium ion binding
D	0032991	cellular_component	protein-containing complex
D	0042802	molecular_function	identical protein binding
D	0044206	biological_process	UMP salvage
E	0003824	molecular_function	catalytic activity
E	0004731	molecular_function	purine-nucleoside phosphorylase activity
E	0004850	molecular_function	uridine phosphorylase activity
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006152	biological_process	purine nucleoside catabolic process
E	0006218	biological_process	uridine catabolic process
E	0006974	biological_process	DNA damage response
E	0009116	biological_process	nucleoside metabolic process
E	0009164	biological_process	nucleoside catabolic process
E	0009166	biological_process	nucleotide catabolic process
E	0016740	molecular_function	transferase activity
E	0016757	molecular_function	glycosyltransferase activity
E	0016763	molecular_function	pentosyltransferase activity
E	0030955	molecular_function	potassium ion binding
E	0032991	cellular_component	protein-containing complex
E	0042802	molecular_function	identical protein binding
E	0044206	biological_process	UMP salvage
F	0003824	molecular_function	catalytic activity
F	0004731	molecular_function	purine-nucleoside phosphorylase activity
F	0004850	molecular_function	uridine phosphorylase activity
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006152	biological_process	purine nucleoside catabolic process
F	0006218	biological_process	uridine catabolic process
F	0006974	biological_process	DNA damage response
F	0009116	biological_process	nucleoside metabolic process
F	0009164	biological_process	nucleoside catabolic process
F	0009166	biological_process	nucleotide catabolic process
F	0016740	molecular_function	transferase activity
F	0016757	molecular_function	glycosyltransferase activity
F	0016763	molecular_function	pentosyltransferase activity
F	0030955	molecular_function	potassium ion binding
F	0032991	cellular_component	protein-containing complex
F	0042802	molecular_function	identical protein binding
F	0044206	biological_process	UMP salvage

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PO4 A 3401

Chain	Residue
A	GLY26
A	ARG30
A	ARG91
A	ILE92
A	GLY93
A	THR94
A	GLU198
A	HOH3407
B	ARG48

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 B 4401

Chain	Residue
A	ARG48
B	GLY26
B	ARG30
B	ARG91
B	ILE92
B	GLY93
B	THR94
B	GLU198
B	HOH4404
B	HOH4509

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PO4 C 5401

Chain	Residue
C	GLY26
C	ARG30
C	ARG91
C	ILE92
C	GLY93
C	THR94
C	GLU198
C	HOH5427
D	ARG48

site_id	AC4
Number of Residues	11
Details	BINDING SITE FOR RESIDUE PO4 D 6401

Chain	Residue
C	ARG48
D	GLY26
D	ARG30
D	ARG91
D	ILE92
D	GLY93
D	THR94
D	GLU198
D	1826400
D	HOH6419
D	HOH6451

site_id	AC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 E 7401

Chain	Residue
E	GLY26
E	ARG30
E	ARG91
E	ILE92
E	GLY93
E	THR94
E	GLU198
E	1827400
E	HOH7402
F	ARG48

site_id	AC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PO4 F 8401

Chain	Residue
E	ARG48
F	GLY26
F	ARG30
F	ARG91
F	ILE92
F	GLY93
F	THR94
F	HOH8404
F	HOH8481

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE K E 1001

Chain	Residue
E	GLU49
E	ILE69
E	SER73
F	GLU49
F	ILE69
F	SER73

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE K A 1002

Chain	Residue
A	GLU49
A	ILE69
A	SER73
B	GLU49
B	ILE69
B	SER73

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE K C 1003

Chain	Residue
C	GLU49
C	ILE69
C	SER73
D	GLU49
D	ILE69
D	SER73

site_id	BC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE 182 A 3400

Chain	Residue
A	THR94
A	GLY96
A	PHE162
A	GLN166
A	ARG168
A	TYR195
A	GLU196
A	MET197
A	GLU227
A	PRO229
A	HOH3440
B	HIS8

site_id	BC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE 182 B 4400

Chain	Residue
B	HOH4480
A	PHE7
A	HIS8
B	THR94
B	THR95
B	GLY96
B	PHE162
B	GLN166
B	ARG168
B	GLU196
B	MET197
B	HOH4422

site_id	BC3
Number of Residues	12
Details	BINDING SITE FOR RESIDUE 182 C 5400

Chain	Residue
C	THR94
C	THR95
C	GLY96
C	PHE162
C	GLN166
C	ARG168
C	TYR195
C	GLU196
C	MET197
C	HOH5405
C	HOH5442
D	HIS8

site_id	BC4
Number of Residues	12
Details	BINDING SITE FOR RESIDUE 182 D 6400

Chain	Residue
C	HIS8
D	THR94
D	THR95
D	GLY96
D	PHE162
D	GLN166
D	ARG168
D	GLU196
D	MET197
D	ILE220
D	PO46401
D	HOH6413

site_id	BC5
Number of Residues	14
Details	BINDING SITE FOR RESIDUE 182 E 7400

Chain	Residue
E	THR94
E	THR95
E	GLY96
E	PHE162
E	GLN166
E	ARG168
E	TYR195
E	GLU196
E	MET197
E	ILE220
E	PO47401
E	HOH7422
E	HOH7431
F	HIS8

site_id	BC6
Number of Residues	14
Details	BINDING SITE FOR RESIDUE 182 F 8400

Chain	Residue
E	PHE7
E	HIS8
F	THR94
F	THR95
F	GLY96
F	PHE162
F	GLN166
F	ARG168
F	TYR195
F	GLU196
F	MET197
F	ILE220
F	HOH8418
F	HOH8436

Functional Information from PROSITE/UniProt

site_id	PS01232
Number of Residues	16
Details	PNP_UDP_1 Purine and other phosphorylases family 1 signature. StGIGgPStSIaveEL

Chain	Residue	Details
A	SER66-LEU81

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 787

Chain	Residue	Details
A	LEU9	proton acceptor, proton donor
A	LEU81	electrostatic stabiliser, polar interaction
A	TYR169	proton acceptor, proton donor
A	THR224	electrostatic stabiliser, polar interaction

site_id	MCSA2
Number of Residues	4
Details	M-CSA 787

Chain	Residue	Details
B	LEU9	proton acceptor, proton donor
B	LEU81	electrostatic stabiliser, polar interaction
B	TYR169	proton acceptor, proton donor
B	THR224	electrostatic stabiliser, polar interaction

site_id	MCSA3
Number of Residues	4
Details	M-CSA 787

Chain	Residue	Details
C	LEU9	proton acceptor, proton donor
C	LEU81	electrostatic stabiliser, polar interaction
C	TYR169	proton acceptor, proton donor
C	THR224	electrostatic stabiliser, polar interaction

site_id	MCSA4
Number of Residues	4
Details	M-CSA 787

Chain	Residue	Details
D	LEU9	proton acceptor, proton donor
D	LEU81	electrostatic stabiliser, polar interaction
D	TYR169	proton acceptor, proton donor
D	THR224	electrostatic stabiliser, polar interaction

site_id	MCSA5
Number of Residues	4
Details	M-CSA 787

Chain	Residue	Details
E	LEU9	proton acceptor, proton donor
E	LEU81	electrostatic stabiliser, polar interaction
E	TYR169	proton acceptor, proton donor
E	THR224	electrostatic stabiliser, polar interaction

site_id	MCSA6
Number of Residues	4
Details	M-CSA 787

Chain	Residue	Details
F	LEU9	proton acceptor, proton donor
F	LEU81	electrostatic stabiliser, polar interaction
F	TYR169	proton acceptor, proton donor
F	THR224	electrostatic stabiliser, polar interaction

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)