1TYB

STRUCTURAL ANALYSIS OF A SERIES OF MUTANTS OF TYROSYL-TRNA SYNTHETASE: ENHANCEMENT OF CATALYSIS BY HYDROPHOBIC INTERACTIONS

Functional Information from GO Data

Chain	GOid	namespace	contents
E	0000166	molecular_function	nucleotide binding
E	0004812	molecular_function	aminoacyl-tRNA ligase activity
E	0004831	molecular_function	tyrosine-tRNA ligase activity
E	0005524	molecular_function	ATP binding
E	0006418	biological_process	tRNA aminoacylation for protein translation
E	0006437	biological_process	tyrosyl-tRNA aminoacylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE TYR E 320

Chain	Residue
E	TYR34
E	GLN195
E	HOH349
E	GLY36
E	ASP38
E	LEU68
E	THR73
E	ASP78
E	TYR169
E	GLN173
E	ASP176

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Functional Information from PROSITE/UniProt

site_id	PS00178
Number of Residues	11
Details	AA_TRNA_LIGASE_I Aminoacyl-transfer RNA synthetases class-I signature. Pt.ADsLHIGHL

Chain	Residue	Details
E	PRO39-LEU49

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	BINDING: BINDING => ECO:0007744|PDB:1TYD

Chain	Residue	Details
E	TYR34
E	TYR169

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site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000250, ECO:0007744|PDB:1TYD

Chain	Residue	Details
E	GLN173
E	ASP176

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site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
E	LYS233

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1d2r

Chain	Residue	Details
E	LYS233
E	LYS230

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site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1d2r

Chain	Residue	Details
E	ARG86
E	LYS82
E	LYS233
E	LYS230

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site_id	MCSA1
Number of Residues	10
Details	M-CSA 197

Chain	Residue	Details
E	THR40	electrostatic stabiliser, hydrogen bond donor
E	THR234	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, metal ligand
E	HIS45	electrostatic stabiliser, hydrogen bond donor
E	HIS48	electrostatic stabiliser, hydrogen bond donor
E	LYS82	electrostatic stabiliser, hydrogen bond donor
E	ARG86	electrostatic stabiliser, hydrogen bond donor
E	GLN173	electrostatic stabiliser, hydrogen bond acceptor
E	ASP194	electrostatic stabiliser, hydrogen bond acceptor
E	LYS230	electrostatic stabiliser, hydrogen bond donor
E	LYS233	electrostatic stabiliser, hydrogen bond donor

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