1TO9

Crystal structure of THI-4 protein from Bacillus subtilis

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005829	cellular_component	cytosol
A	0006725	biological_process	obsolete cellular aromatic compound metabolic process
A	0006772	biological_process	thiamine metabolic process
A	0006790	biological_process	sulfur compound metabolic process
A	0009228	biological_process	thiamine biosynthetic process
A	0009229	biological_process	thiamine diphosphate biosynthetic process
A	0016787	molecular_function	hydrolase activity
A	0044281	biological_process	small molecule metabolic process
A	0050334	molecular_function	thiaminase activity
A	1901360	biological_process	obsolete organic cyclic compound metabolic process
B	0005829	cellular_component	cytosol
B	0006725	biological_process	obsolete cellular aromatic compound metabolic process
B	0006772	biological_process	thiamine metabolic process
B	0006790	biological_process	sulfur compound metabolic process
B	0009228	biological_process	thiamine biosynthetic process
B	0009229	biological_process	thiamine diphosphate biosynthetic process
B	0016787	molecular_function	hydrolase activity
B	0044281	biological_process	small molecule metabolic process
B	0050334	molecular_function	thiaminase activity
B	1901360	biological_process	obsolete organic cyclic compound metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE HMH A 301

Chain	Residue
A	ASP44
A	TYR47
A	ALA81
A	LEU85
A	CYS135
A	TYR139
A	TYR163
A	GLU205

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site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE HMH B 302

Chain	Residue
B	TYR47
B	CYS135
B	TYR139
B	TYR163
B	GLU205
B	ASP44

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000303|PubMed:18054064

Chain	Residue	Details
B	CYS135

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000303|PubMed:18054064

Chain	Residue	Details
B	GLU205

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site_id	SWS_FT_FI3
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:15709744, ECO:0000269|Ref.5

Chain	Residue	Details
B	ASP44
B	TYR139
B	TYR163

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site_id	SWS_FT_FI4
Number of Residues	1
Details	SITE: Increases nucleophilicity of active site Cys => ECO:0000303|PubMed:18054064

Chain	Residue	Details
B	TYR47

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