1TMN

Binding of n-carboxymethyl dipeptide inhibitors to thermolysin determined by x-ray crystallography. a novel class of transition-state analogues for zinc peptidases

Functional Information from GO Data

Chain	GOid	namespace	contents
E	0004222	molecular_function	metalloendopeptidase activity
E	0006508	biological_process	proteolysis

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE 0ZN E 317

Chain	Residue
E	TYR110
E	GLU166
E	LEU202
E	ARG203
E	HIS231
E	ZN322
E	HOH362
E	ASN111
E	ASN112
E	ALA113
E	VAL139
E	HIS142
E	GLU143
E	HIS146
E	TYR157

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA E 318

Chain	Residue
E	ASP138
E	GLU177
E	ASP185
E	GLU187
E	GLU190
E	HOH346

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA E 319

Chain	Residue
E	GLU177
E	ASN183
E	ASP185
E	GLU190
E	HOH353
E	HOH475

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA E 320

Chain	Residue
E	ASP57
E	ASP59
E	GLN61
E	HOH419
E	HOH482

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA E 321

Chain	Residue
E	TYR193
E	THR194
E	ILE197
E	ASP200
E	HOH354
E	HOH480

---

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN E 322

Chain	Residue
E	HIS142
E	HIS146
E	GLU166
E	0ZN317

---

site_id	S1
Number of Residues	1
Details	SUBSITE 1 OF THE ACTIVE SITE CLEFT OF THERMOLYSIN

Chain	Residue
E	PHE114

---

site_id	S1P
Number of Residues	7
Details	SUBSITE 2 OF THE ACTIVE SITE CLEFT OF THERMOLYSIN

Chain	Residue
E	PHE130
E	LEU133
E	VAL139
E	ILE188
E	GLY189
E	VAL192
E	LEU202

---

site_id	S2
Number of Residues	1
Details	SUBSITE 3 OF THE ACTIVE SITE CLEFT OF THERMOLYSIN

Chain	Residue
E	TRP115

---

site_id	S2P
Number of Residues	2
Details	SUBSITE 4 OF THE ACTIVE SITE CLEFT OF THERMOLYSIN

Chain	Residue
E	PHE130
E	LEU202

---

Functional Information from PROSITE/UniProt

site_id	PS00142
Number of Residues	10
Details	ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. VVAHELTHAV

Chain	Residue	Details
E	VAL139-VAL148

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU10095","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"4808703","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PROSITE-ProRule","id":"PRU10095","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"4808703","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1tlp

Chain	Residue	Details
E	HIS231
E	GLU143

---

site_id	MCSA1
Number of Residues	7
Details	M-CSA 176

Chain

Residue

Details

---