1SD9

ARSENATE REDUCTASE C12S MUTANT +0.4M ARSENATE FROM E. COLI

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008794	molecular_function	arsenate reductase (glutaredoxin) activity
A	0016491	molecular_function	oxidoreductase activity
A	0046685	biological_process	response to arsenic-containing substance

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SO4 A 201

Chain	Residue
A	SER12
A	HOH1090
A	GLY13
A	THR14
A	ARG60
A	ASN62
A	ARG94
A	ARG107
A	HOH1008
A	HOH1076

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site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 202

Chain	Residue
A	ARG107
A	HOH1062
A	HOH1101
A	HOH1223

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site_id	AC3
Number of Residues	12
Details	BINDING SITE FOR RESIDUE SO4 A 203

Chain	Residue
A	THR14
A	ASN17
A	PRO108
A	SER109
A	GLU110
A	LYS126
A	HOH1008
A	HOH1023
A	HOH1087
A	HOH1093
A	HOH1193
A	HOH1213

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site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CS A 401

Chain	Residue
A	LEU113
A	LEU113
A	ASP114
A	ASP114
A	LEU116
A	LEU116

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site_id	AC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CS A 402

Chain	Residue
A	PRO38
A	GLU43

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile; cysteine thioarsenate intermediate => ECO:0000255|PROSITE-ProRule:PRU01282, ECO:0000269|PubMed:11709171, ECO:0000269|PubMed:15295115, ECO:0000269|PubMed:7577935

Chain	Residue	Details
A	SER12

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site_id	SWS_FT_FI2
Number of Residues	1
Details	SITE: Important for activity. Lowers pKa of the active site Cys => ECO:0000269|PubMed:8969183

Chain	Residue	Details
A	HIS8

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site_id	SWS_FT_FI3
Number of Residues	3
Details	SITE: Important for activity. Involved in arsenate binding and transition-state stabilization => ECO:0000269|PubMed:11709171, ECO:0000269|PubMed:14592722, ECO:0000269|PubMed:15295115

Chain	Residue	Details
A	ARG60
A	ARG94
A	ARG107

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