Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0009257 | biological_process | 10-formyltetrahydrofolate biosynthetic process |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0031427 | biological_process | response to methotrexate |
| A | 0044281 | biological_process | small molecule metabolic process |
| A | 0046452 | biological_process | dihydrofolate metabolic process |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0046656 | biological_process | folic acid biosynthetic process |
| A | 0046677 | biological_process | response to antibiotic |
| A | 0050661 | molecular_function | NADP binding |
| A | 0051870 | molecular_function | methotrexate binding |
| A | 0051871 | molecular_function | dihydrofolic acid binding |
| A | 0070401 | molecular_function | NADP+ binding |
| A | 0070402 | molecular_function | NADPH binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE CA A 300 |
| Chain | Residue |
| A | ASP116 |
| A | HIS149 |
| A | ARG159 |
| A | HOH310 |
| A | HOH370 |
| A | HOH390 |
| A | HOH391 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE BME A 162 |
| Chain | Residue |
| A | SER138 |
| A | CYS152 |
| site_id | AC3 |
| Number of Residues | 28 |
| Details | BINDING SITE FOR RESIDUE NAP A 164 |
| Chain | Residue |
| A | ALA6 |
| A | ALA7 |
| A | ILE14 |
| A | GLY15 |
| A | MET16 |
| A | ASN18 |
| A | ALA19 |
| A | GLY43 |
| A | ARG44 |
| A | HIS45 |
| A | THR46 |
| A | LEU62 |
| A | SER63 |
| A | SER64 |
| A | GLN65 |
| A | LYS76 |
| A | ILE94 |
| A | GLY95 |
| A | GLY96 |
| A | GLY97 |
| A | ARG98 |
| A | VAL99 |
| A | TYR100 |
| A | GLN102 |
| A | ASP122 |
| A | ASP131 |
| A | HOH362 |
| A | HOH383 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGmenaMPWnlpa.DlawFkrnT |
| Chain | Residue | Details |
| A | VAL13-THR35 | |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 157 |
| Details | Domain: {"description":"DHFR","evidences":[{"source":"PROSITE-ProRule","id":"PRU00660","evidenceCode":"ECO:0000255"}]} |
| site_id | SWS_FT_FI2 |
| Number of Residues | 5 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"9012674","evidenceCode":"ECO:0000305"}]} |
| site_id | SWS_FT_FI3 |
| Number of Residues | 17 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"19374017","evidenceCode":"ECO:0000269"}]} |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 7 |
| Details | Annotated By Reference To The Literature 1ra2 |
| Chain | Residue | Details |
| A | LEU54 | |
| A | LEU28 | |
| A | PHE31 | |
| A | ASP27 | |
| A | ILE5 | |
| A | MET20 | |
| A | ILE94 | |
