1RRJ

Structural Mechanisms of Camptothecin Resistance by Mutations in Human Topoisomerase I

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003917	molecular_function	DNA topoisomerase type I (single strand cut, ATP-independent) activity
A	0005694	cellular_component	chromosome
A	0006265	biological_process	DNA topological change

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE TTG B 991

Chain	Residue
A	GLU356
B	HOH2180
B	HOH2217
B	HOH2293
B	HOH2319
C	DC112
C	DA113
A	ARG364
A	LYS532
A	ASP533
A	THR718
B	DT10
B	DG11
B	TTC990
B	HOH2001

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site_id	AC2
Number of Residues	15
Details	BINDING SITE FOR RESIDUE TTC B 990

Chain	Residue
A	GLU356
A	ARG364
A	LYS532
A	ASP533
A	THR718
A	PTR723
B	DT10
B	DG11
B	TTG991
B	HOH2001
B	HOH2180
B	HOH2217
B	HOH2293
C	DC112
C	DA113

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Functional Information from PROSITE/UniProt

site_id	PS00176
Number of Residues	19
Details	TOPO_IB_1 Topoisomerase (Topo) IB-type active site signature. EnkqialGTSKlsYLdprI

Chain	Residue	Details
A	GLU710-ILE728

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: O-(3'-phospho-DNA)-tyrosine intermediate => ECO:0000255|PROSITE-ProRule:PRU01382, ECO:0000255|PROSITE-ProRule:PRU10130, ECO:0000269|PubMed:10841763, ECO:0000269|PubMed:12426403, ECO:0000269|PubMed:12533542, ECO:0000269|PubMed:15165849, ECO:0000269|PubMed:15801827, ECO:0000269|PubMed:16033260, ECO:0000269|PubMed:9488644

Chain	Residue	Details
A	PTR723

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site_id	SWS_FT_FI2
Number of Residues	9
Details	SITE: Interaction with DNA

Chain	Residue	Details
A	ARG316
A	ARG364
A	TRP412
A	LYS443
A	THR501
A	LYS532
A	ASN574
A	HIS632
A	LYS650

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site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
A	LYS280

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site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine; by CK2 => ECO:0000269|PubMed:23185622

Chain	Residue	Details
A	SER506

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site_id	SWS_FT_FI5
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS204
A	LYS549
A	LYS642
A	LYS700
A	LYS712

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site_id	SWS_FT_FI6
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS336

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1k4t

Chain	Residue	Details
A	HIS632
A	ARG488
A	ARG590

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