1RAH
CRYSTAL STRUCTURE OF CTP-LIGATED T STATE ASPARTATE TRANSCARBAMOYLASE AT 2.5 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ATCASE MUTANTS AND THE MECHANISM OF NEGATIVE COOPERATIVITY
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004070 | molecular_function | aspartate carbamoyltransferase activity |
| A | 0004088 | molecular_function | carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| A | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| A | 0006520 | biological_process | amino acid metabolic process |
| A | 0006541 | biological_process | glutamine metabolic process |
| A | 0009347 | cellular_component | aspartate carbamoyltransferase complex |
| A | 0016597 | molecular_function | amino acid binding |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016743 | molecular_function | carboxyl- or carbamoyltransferase activity |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0044205 | biological_process | 'de novo' UMP biosynthetic process |
| A | 0070207 | biological_process | protein homotrimerization |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| B | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0009347 | cellular_component | aspartate carbamoyltransferase complex |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0004070 | molecular_function | aspartate carbamoyltransferase activity |
| C | 0004088 | molecular_function | carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| C | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| C | 0006520 | biological_process | amino acid metabolic process |
| C | 0006541 | biological_process | glutamine metabolic process |
| C | 0009347 | cellular_component | aspartate carbamoyltransferase complex |
| C | 0016597 | molecular_function | amino acid binding |
| C | 0016740 | molecular_function | transferase activity |
| C | 0016743 | molecular_function | carboxyl- or carbamoyltransferase activity |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0044205 | biological_process | 'de novo' UMP biosynthetic process |
| C | 0070207 | biological_process | protein homotrimerization |
| D | 0005515 | molecular_function | protein binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| D | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| D | 0008270 | molecular_function | zinc ion binding |
| D | 0009347 | cellular_component | aspartate carbamoyltransferase complex |
| D | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 154 |
| Chain | Residue |
| B | CYS114 |
| B | CYS138 |
| B | CYS141 |
| B | CYS109 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN D 154 |
| Chain | Residue |
| D | CYS109 |
| D | CYS114 |
| D | CYS138 |
| D | CYS141 |
| site_id | AC3 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE CTP B 999 |
| Chain | Residue |
| B | VAL9 |
| B | ALA11 |
| B | ILE12 |
| B | HIS20 |
| B | LYS60 |
| B | ILE86 |
| B | TYR89 |
| B | LYS94 |
| site_id | AC4 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE CTP D 999 |
| Chain | Residue |
| D | GLU10 |
| D | ALA11 |
| D | ILE12 |
| D | ASP19 |
| D | THR82 |
| D | ASN84 |
| D | TYR89 |
| D | GLU90 |
| D | VAL91 |
| D | LYS94 |
| site_id | ZNB |
| Number of Residues | 4 |
| Details | ZN BINDING SITE OF CHAIN B |
| Chain | Residue |
| B | CYS109 |
| B | CYS114 |
| B | CYS138 |
| B | CYS141 |
| site_id | ZND |
| Number of Residues | 4 |
| Details | ZN BINDING SITE OF CHAIN D |
| Chain | Residue |
| D | CYS109 |
| D | CYS114 |
| D | CYS138 |
| D | CYS141 |
Functional Information from PROSITE/UniProt
| site_id | PS00097 |
| Number of Residues | 8 |
| Details | CARBAMOYLTRANSFERASE Aspartate and ornithine carbamoyltransferases signature. FfEaSTRT |
| Chain | Residue | Details |
| A | PHE48-THR55 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | BINDING: |
| Chain | Residue | Details |
| B | CYS109 | |
| C | ARG105 | |
| C | HIS134 | |
| C | GLN137 | |
| C | LEU267 | |
| C | PRO268 | |
| B | CYS114 | |
| B | CYS138 | |
| B | CYS141 | |
| D | CYS109 | |
| D | CYS114 | |
| D | CYS138 | |
| D | CYS141 | |
| C | THR55 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00001, ECO:0000305|PubMed:3380787 |
| Chain | Residue | Details |
| A | LYS84 | |
| A | ARG167 | |
| A | ARG229 | |
| C | LYS84 | |
| C | ARG167 | |
| C | ARG229 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1at1 |
| Chain | Residue | Details |
| A | ARG54 | |
| A | HIS134 | |
| A | ARG105 | |
| A | THR55 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1at1 |
| Chain | Residue | Details |
| C | ARG54 | |
| C | HIS134 | |
| C | ARG105 | |
| C | THR55 |
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 405 |
| Chain | Residue | Details |
| A | ARG54 | electrostatic stabiliser |
| A | THR55 | electrostatic stabiliser, increase electrophilicity |
| A | LYS84 | proton shuttle (general acid/base) |
| A | ARG105 | electrostatic stabiliser, increase electrophilicity |
| A | HIS134 | electrostatic stabiliser, increase electrophilicity |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 405 |
| Chain | Residue | Details |
| C | ARG54 | electrostatic stabiliser |
| C | THR55 | electrostatic stabiliser, increase electrophilicity |
| C | LYS84 | proton shuttle (general acid/base) |
| C | ARG105 | electrostatic stabiliser, increase electrophilicity |
| C | HIS134 | electrostatic stabiliser, increase electrophilicity |
