1QHO

FIVE-DOMAIN ALPHA-AMYLASE FROM BACILLUS STEAROTHERMOPHILUS, MALTOSE/ACARBOSE COMPLEX

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004556	molecular_function	alpha-amylase activity
A	0005975	biological_process	carbohydrate metabolic process
A	0030246	molecular_function	carbohydrate binding
A	0043169	molecular_function	cation binding
A	2001070	molecular_function	starch binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	76
Details	Domain: {"description":"IPT/TIG"}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	110
Details	Domain: {"description":"CBM20","evidences":[{"source":"PROSITE-ProRule","id":"PRU00594","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	1
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"UniProtKB","id":"P13507","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	1
Details	Active site: {"description":"Proton donor","evidences":[{"source":"UniProtKB","id":"P13507","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI5
Number of Residues	15
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10387084","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1QHO","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI6
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P0C1B3","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Site: {"description":"Transition state stabilizer","evidences":[{"source":"UniProtKB","id":"P0C1B3","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details

Chain	Residue	Details
A	ASP228
A	ASP329
A	GLU256
A	HIS132

---

site_id	MCSA1
Number of Residues	3
Details	M-CSA 905

Chain	Residue	Details
A	ASP228	covalent catalysis
A	GLU256	proton shuttle (general acid/base)
A	ASP329	transition state stabiliser

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