1QFM

PROLYL OLIGOPEPTIDASE FROM PORCINE MUSCLE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004252	molecular_function	serine-type endopeptidase activity
A	0005737	cellular_component	cytoplasm
A	0006508	biological_process	proteolysis
A	0008233	molecular_function	peptidase activity
A	0008236	molecular_function	serine-type peptidase activity
A	0016787	molecular_function	hydrolase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SGL A 781

Chain	Residue
A	TYR473
A	SER554
A	ASN555
A	VAL580
A	TRP595
A	HIS680
A	HOH838

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SGL A 782

Chain	Residue
A	TRP595
A	ARG643
A	HOH838
A	HOH1473
A	HOH1586
A	CSX255
A	ILE591

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SGM A 783

Chain	Residue
A	CYS78
A	HIS79
A	PHE80
A	TYR385
A	SER386
A	HOH1212
A	HOH1460

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SGM A 784

Chain	Residue
A	PRO443
A	CYS532
A	GLU535

---

site_id	AC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 790

Chain	Residue
A	ILE118
A	LEU119
A	SER120
A	ASP121
A	ASP446
A	ASN522
A	ASN525
A	HOH1163
A	HOH1418

---

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 791

Chain	Residue
A	ALA226
A	GLU227
A	PHE228
A	TRP262
A	ILE276
A	LYS281
A	HOH1238
A	HOH1529

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 792

Chain	Residue
A	TRP150
A	VAL151
A	GLU169
A	ARG170
A	VAL171
A	SER197
A	HOH1021

---

site_id	AC8
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GOL A 793

Chain	Residue
A	PRO7
A	ASP8
A	VAL9
A	TRP30
A	GLN38
A	ALA41
A	PHE42
A	HOH929
A	HOH959
A	HOH1412
A	HOH1507

---

Functional Information from PROSITE/UniProt

site_id	PS00708
Number of Residues	31
Details	PRO_ENDOPEP_SER Prolyl endopeptidase family serine active site. DfqcAaeyLikegytspkrltinGgSnGGLL

Chain	Residue	Details
A	ASP529-LEU559

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"PROSITE-ProRule","id":"PRU10084","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1900195","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Charge relay system"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"PROSITE-ProRule","id":"PRU10084","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"2064618","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"UniProtKB","id":"P48147","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P48147","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	a catalytic site defined by CSA, PubMed 9695945, 10716187, 11915948, 12202494, 12228249

Chain	Residue	Details
A	ASP641
A	SER554
A	HIS680

---

site_id	MCSA1
Number of Residues	4
Details	M-CSA 901

Chain	Residue	Details
A	LEU489	electrostatic stabiliser
A	LEU570	covalent catalysis, proton shuttle (general acid/base)
A	GLY661	electrostatic stabiliser, modifies pKa
A	ILE700	proton shuttle (general acid/base)

---