1P3W

X-ray crystal structure of E. coli IscS

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002143	biological_process	tRNA wobble position uridine thiolation
A	0002937	biological_process	tRNA 4-thiouridine biosynthesis
A	0003824	molecular_function	catalytic activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0008033	biological_process	tRNA processing
A	0009000	molecular_function	selenocysteine lyase activity
A	0009228	biological_process	thiamine biosynthetic process
A	0009589	biological_process	detection of UV
A	0016226	biological_process	iron-sulfur cluster assembly
A	0016261	biological_process	selenocysteine catabolic process
A	0016740	molecular_function	transferase activity
A	0018131	biological_process	oxazole or thiazole biosynthetic process
A	0019448	biological_process	L-cysteine catabolic process
A	0030170	molecular_function	pyridoxal phosphate binding
A	0031071	molecular_function	cysteine desulfurase activity
A	0044571	biological_process	[2Fe-2S] cluster assembly
A	0046872	molecular_function	metal ion binding
A	0051536	molecular_function	iron-sulfur cluster binding
A	0051537	molecular_function	2 iron, 2 sulfur cluster binding
A	0072348	biological_process	sulfur compound transport
A	0097163	molecular_function	sulfur carrier activity
A	1990221	cellular_component	L-cysteine desulfurase complex
A	1990228	cellular_component	sulfurtransferase complex
A	1990329	cellular_component	IscS-TusA complex
A	1990330	cellular_component	IscS-IscU complex
B	0002143	biological_process	tRNA wobble position uridine thiolation
B	0002937	biological_process	tRNA 4-thiouridine biosynthesis
B	0003824	molecular_function	catalytic activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0008033	biological_process	tRNA processing
B	0009000	molecular_function	selenocysteine lyase activity
B	0009228	biological_process	thiamine biosynthetic process
B	0009589	biological_process	detection of UV
B	0016226	biological_process	iron-sulfur cluster assembly
B	0016261	biological_process	selenocysteine catabolic process
B	0016740	molecular_function	transferase activity
B	0018131	biological_process	oxazole or thiazole biosynthetic process
B	0019448	biological_process	L-cysteine catabolic process
B	0030170	molecular_function	pyridoxal phosphate binding
B	0031071	molecular_function	cysteine desulfurase activity
B	0044571	biological_process	[2Fe-2S] cluster assembly
B	0046872	molecular_function	metal ion binding
B	0051536	molecular_function	iron-sulfur cluster binding
B	0051537	molecular_function	2 iron, 2 sulfur cluster binding
B	0072348	biological_process	sulfur compound transport
B	0097163	molecular_function	sulfur carrier activity
B	1990221	cellular_component	L-cysteine desulfurase complex
B	1990228	cellular_component	sulfurtransferase complex
B	1990329	cellular_component	IscS-TusA complex
B	1990330	cellular_component	IscS-IscU complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	BINDING SITE FOR RESIDUE PLP B 764

Chain	Residue
A	THR243
B	GLN183
B	SER203
B	HIS205
B	LYS206
B	HOH795
B	HOH848
B	HOH925
A	HOH880
B	ALA75
B	THR76
B	HIS104
B	MET151
B	ASN155
B	ASP180
B	THR182

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site_id	AC2
Number of Residues	15
Details	BINDING SITE FOR RESIDUE PLP A 764

Chain	Residue
A	ALA75
A	THR76
A	HIS104
A	MET151
A	ASN155
A	ASP180
A	THR182
A	GLN183
A	SER203
A	HIS205
A	LYS206
A	HOH801
A	HOH823
A	HOH895
B	THR243

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Functional Information from PROSITE/UniProt

site_id	PS00595
Number of Residues	20
Details	AA_TRANSFER_CLASS_5 Aminotransferases class-V pyridoxal-phosphate attachment site. VDLMsfSGHKiygpk.GiGaL

Chain	Residue	Details
B	VAL197-LEU216

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Cysteine persulfide intermediate => ECO:0000255|HAMAP-Rule:MF_00331, ECO:0000269|PubMed:12860127, ECO:0000269|PubMed:8663056

Chain	Residue	Details
B	CYS328
A	CYS328

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site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00331

Chain	Residue	Details
A	ALA75
A	ASN155
B	ALA75
B	ASN155

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00331, ECO:0000269|PubMed:12860127

Chain	Residue	Details
B	THR243
A	GLN183
A	SER203
A	THR243
B	GLN183
B	SER203

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site_id	SWS_FT_FI4
Number of Residues	2
Details	BINDING: via persulfide group => ECO:0000255|HAMAP-Rule:MF_00331

Chain	Residue	Details
B	CYS328
A	CYS328

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site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000255|HAMAP-Rule:MF_00331, ECO:0000269|PubMed:12860127

Chain	Residue	Details
B	LYS206
A	LYS206

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